CAZyme Information: MGYG000000555_00812

Basic Information

• Species: Cellulosilyticum sp900556665
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Cellulosilyticaceae; Cellulosilyticum; Cellulosilyticum sp900556665
• CAZyme ID: MGYG000000555_00812
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1185		135097.82	4.7549

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000555	2866181	MAG	China	Asia

• Gene Location: Start: 5664; End: 9221 Strand: -

Full Sequence      

MSRKILFNESWFFTKQPLDTTLQEVNERDDAFKSVTIPHDWLIYDANNLYENSTGWYKKR
FILQKGEGAKYFIRFDGVYMDSTVYVNDIKIGEWKYGYSAFEMDLTEALVNGENEIKVSV
RYESPNSRWYSGAGIFRDVWFKEVSSTYLVSDGIYVTALPIDETLDDETIWQVEVDTEVI
CKEAATLSYAIIHKNSGEKLALYQDKTAAMQGEKVDTKGVNTTSEFINIQQAEGVQSVAH
TFYVKGPKLWELEAPNCYSLCICLRKGDETLQEEVVQFGFRTIEYKVDGGFYLNHKHVKL
RGVCEHHDLGCLGAAYNEAAMRRKFENLRAMGINAVRTSHNMSAENMMYLADELGFLIVA
EGFDIWEKAKTTYDYARFFDDWYKKDVASWVRRDRNHPSVIMWSIGNEIYDTHESDRGQE
VTRMLLKEVKKHDPKNHAPVTIGSNYMPWENAQKCADILKMAGYNYGEKFYDAHHEKYKD
WVIYGSETASVVQSRGIYHFPLSHSILMDDDEQCSALGNSTTVWGAKNHEFCIIHERDTH
YSCGQFLWSGHDYIGEPTPYQTKNSYFGQLDTAGFPKDSFYIYQAEWTDYKKSPMVHLFP
YWDFNEGELIDIRICSNAPKVELFLNGISQGTYEIDHAHGKELLGNWQIPYTKGEIEAVA
YDENGNIIARDSHKSFTEPTQIIMKPNKTEIIADEKDLVFIEITMLDENNNPVENANNRV
QIEVSEGGILMGLDNGDSTDYDSYKGSSKRLFSGKLLAVVAVDKAYQGDKVTIKASSVGL
PDTTIDLQALGTGLQNSQSRIDMGTGLQYSQSEEWKLQEVPIRKIEIISPRGTELNESLR
ELEIIAKIYPENATYNEITWQVVDDAVIPSHLAVIEPMLVENGEDTTNQKVFKVRLKALG
DGNIRVRCLAKNGKEVGCLISQLNFTITGIGTAYLNPYEFISGGVYDFTKGEVASGNEKG
FAIAKDGETIIGFSNIDFGAYGSDEITIPIFALDDRAYNIEIWEGMPYETGSIKVAQVVY
QKPSIWNTYQEETYKLNKRIQGVSSIYFVFKDKVHVKGFVFKSYNRALQQLNVNECDYIY
GDQFNITKDAINEIGNNVTIAFDKMDFGEEGVSKLTICGHSPIDKNSIHLRSNSGEEEEK
QLLEFLYSEGEVIRTYEIKRLTGVQDISFVFLPGSNFNFKWFKFE

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	583	2.5e-104	0.5957446808510638

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.34e-63	7	603	30	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	4.36e-40	56	447	68	452	beta-D-glucuronidase; Provisional
PRK10340	ebgA	5.94e-36	51	408	107	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	8.14e-36	9	408	72	462	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	9.43e-22	288	441	5	159	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACZ98666.1	0.0	1	1185	1	1163
APO44079.1	0.0	1	1185	1	1156
QOS77051.1	0.0	1	1185	1	1168
QZN78011.1	0.0	1	1185	1	1161
ASU31293.1	0.0	5	1184	1	1144

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	4.24e-161	3	787	2	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.46e-154	3	780	8	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	8.74e-137	5	788	28	821	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CU6_A	9.50e-136	3	777	14	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	2.58e-135	3	777	14	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.26e-139	7	789	47	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.02e-91	7	780	51	830	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.04e-54	8	660	57	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	2.99e-43	1	779	1	742	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P26257	8.88e-29	3	732	2	706	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000071	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000555_00812.