CAZyme Information: MGYG000000562_01587

Basic Information

• Species: Lachnospira sp000437735
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnospira; Lachnospira sp000437735
• CAZyme ID: MGYG000000562_01587
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1215	MGYG000000562_6|CGC1	129806.64	4.4228

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000562	2986625	MAG	China	Asia

• Gene Location: Start: 81290; End: 84937 Strand: -

Full Sequence      

MKMKTNRKVKKLFSYVLTAAMVITAVTPAFAGKVKAVAAGLTLTEQSGWLESAYVEWQPV
KNAEGYVAYVKEASASDDAYEKLDDTLIRQYADYWRADALGLKEGSYVMKVTAVLKDGKT
VSESTSSLEVEKYDRSGFAFSENSKFQTASGAYNDDGTLKKDAQVIYVTPETAKTCTAVV
NGKEVTGFQSILDAKQSAGTKDTSSLDFRIVGCVTADDVDHFSSSAEGIQLKGKSAYTEM
NITIEGVGEDAAVHGFGFLVRNSGNVEFRNFAVMAFMDDGVSLDTKNCNIWVHNMDIFYG
STGGDSDQAKGDGSVDIKGASTNVTVSYVHFWDSGKCSLCGMSDSAEFLVTYHHNWFDHS
DSRHPRIRVASVHIYNNYFDGNAKYGVGTTKGSSAFVEANYYRNCKNPMMSSMQGTDALG
QGTFSGENGGMIKAYNNITVGASSLIYANSDAGTAKADAVSFDAYLASSREETVPSSYKT
VAGATTYNNFDTSSAYDLGVAEEDIDDPQDVPSIVTKYAGRMNGGDFDWTFQESDDTNYS
VDTALKAKVVNYKSSVLAIGGYKGVDVEPSTTEKATESTTKATESTTKATEATTKATEST
TKATEATTKATESTTEATTEATTTAPAETTAKVHNFSTDGTSSDFYTISGKLSSNKGTVS
YNGLSLDTCLKIESKTKITFTTTDKAELVLVFNQKNSSDIKVDGTVYTLTDGILSLEIEA
GSHEITKESTGNLYYMSVSQQSETPTTPVTPTEPTQPEQPTTPSEPETPTTPSEPEQTKC
DLYVSPSGKSTAAGTQNAPMDLLTAINSISAGYTIWMEEGTYKAYELYGAPIVIAESNSG
AEDAYKTISSINGGTVTIDFSGMAELGSNRGIVLDGSYWHFYDIDICNAGDNGMLLSGDN
NIIELCQFYANHDSGLQISRYNTSADTIDLWPSNNLILNCTAFDNKDEATCENADGFAAK
LTCGEGNVFDGCISYCNSDDGWDLYAKPATGSTGVVTIKNSVAFGNGKLTNGEGSANGDM
NGFKLGGSNKQCPTPHVVTNCIAFNNGATGFTDNGNGGAVTLTNCTSVNNGMYNAAKANY
MCYRTSKDAAYTNLLSFAEKKTMATDQFLGTVSHTLYHYKGVGTYWVNNWLCTDGAKTKY
SGSEGADYTVSASDFVSTQIPGYDAATGAYTADYHSIFRNADGSINLSGLYEVSSDSPLY
TAGTQNTALGASFSK

Enzyme Prediction     

No EC number prediction in MGYG000000562_01587.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	778	1198	6.2e-122	0.9866666666666667
PL1	236	404	1.8e-42	0.8267326732673267
CBM77	633	734	2.7e-39	0.9805825242718447

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	6.79e-59	135	520	34	340	Pectate lyase [Carbohydrate transport and metabolism].
pfam18283	CBM77	5.41e-37	633	736	4	108	Carbohydrate binding module 77. This domain is the non-catalytic carbohydrate binding module 77 (CBM77) present in Ruminococcus flavefaciens. CBMs fulfil a critical targeting function in plant cell wall depolymerisation. In CBM77, a cluster of conserved basic residues (Lys1092, Lys1107 and Lys1162) confer calcium-independent recognition of homogalacturonan.
smart00656	Amb_all	1.20e-30	241	406	17	190	Amb_all domain.
pfam00544	Pec_lyase_C	3.01e-18	239	402	33	211	Pectate lyase. This enzyme forms a right handed beta helix structure. Pectate lyase is an enzyme involved in the maceration and soft rotting of plant tissue.
pfam13229	Beta_helix	7.37e-05	871	1066	2	157	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACR72246.1	0.0	1	1117	1	1160
ABX41986.1	2.59e-311	10	1213	7	1165
ACR72247.1	2.34e-309	1	1192	1	1043
ADL51369.1	1.34e-288	4	1212	6	1250
AOR96287.1	1.30e-268	41	1213	55	1095

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RU4_A	4.68e-38	778	1212	13	388	ChainA, Pectate lyase [Dickeya chrysanthemi]
5FU5_A	1.06e-22	633	736	9	111	Thecomplexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition [Ruminococcus flavefaciens]
3VMV_A	2.63e-21	232	404	72	248	Crystalstructure of pectate lyase Bsp165PelA from Bacillus sp. N165 [Bacillus sp. N16-5],3VMW_A Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165 in complex with trigalacturonate [Bacillus sp. N16-5]
1VBL_A	1.67e-18	237	402	129	330	Structureof the thermostable pectate lyase PL 47 [Bacillus sp. TS-47]
1AIR_A	5.26e-16	148	428	10	282	ChainA, PECTATE LYASE C [Dickeya chrysanthemi],1O88_A Chain A, Pectate Lyase C [Dickeya chrysanthemi],1O8D_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8E_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8F_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8G_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8H_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8I_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8J_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8K_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8L_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8M_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1PLU_A Chain A, Protein (pectate Lyase C) [Dickeya chrysanthemi],2PEC_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C1A6	2.82e-38	778	1212	38	413	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1
P0C1A7	4.23e-37	778	1212	38	413	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
P22751	2.88e-36	781	1117	390	686	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
Q8GCB2	5.58e-21	223	411	96	281	Pectate trisaccharide-lyase OS=Bacillus licheniformis OX=1402 GN=pelA PE=1 SV=1
Q65DC2	5.58e-21	223	411	96	281	Pectate trisaccharide-lyase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi04129 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000346	0.998820	0.000205	0.000238	0.000185	0.000153

TMHMM  Annotations     

start	end
12	31