CAZyme Information: MGYG000000572_01672

Basic Information

• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; Zag1
• CAZyme ID: MGYG000000572_01672
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
717		80088.89	9.427

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000572	1867050	MAG	Madagascar	Africa

• Gene Location: Start: 118; End: 2271 Strand: +

Full Sequence      

MSRYYVKRNYTAKEMAKANISKSSKRKNGGFFSTLKFIFIMSCACALAGVASLELYLSSL
PPINNLEQFKPNIVTKIYSDDGEIIKTFTAYTYEKIDLKDIPDNLKKALIATEDKNFYRH
HGYDIAGLARSTIQNVLAGHVVQGASTITQQLARVLFLNNERTFDRKLKELFIAARIEKT
ISKDQILEMYMNNVYLGAGAYGVEGAAQIYFDKHLKNCDLAELALIAGLPQAPSVYNPFN
NMDLAVQRRNQVLTRMYKMRYITTEEYEKAKEEKVHLAKVPQYYTTNKAPYFCDYVMKEL
EKLGFDETEISQGGYKVVTTLDYKAQKTANEAILKNLRNWGLNNDKNQAAVFSFSPIDGK
ILVYSGGKDYTKSQYDRVTQAVRPPGSSFKPIVYAAAMEKGISPNDMIEDRPLTIGQWTP
RNYANKYRGKIPVYTALMISSNVCAARLIKDVGIRSVIQTARVLGIETPLEYDYTIALGS
NGVKLFEFTRAYGAFANGGYVVQPYAIERVETSRGKVVYKAPKTKITHQLSMNTAAEMTA
MMKMVISNGTGRAANIGRPAAGKTGTTDDNKDAYFMGYTPNIVTGVWVGNDDNSVMNKSI
QGGSVPALIWKDIMKVATEPYGKAEFNYPQIELMPFGAIHPDKVIGEEAVKPKQEEKEEI
DLNETEPVLPESIRKIEHEQSQPKQAQTPTPKPVTAPKQQNTSAPIPIPMAVPEGVR

Enzyme Prediction     

No EC number prediction in MGYG000000572_01672.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	81	257	8.2e-67	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	0.0	35	701	5	793	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG0744	MrcB	0.0	30	619	12	598	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	94	619	3	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
TIGR02071	PBP_1b	1.51e-111	56	618	113	683	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11636	mrcA	1.06e-107	35	619	4	786	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR38680.1	0.0	1	717	1	716
ACI19842.1	8.49e-151	8	628	2	619
ACK42407.1	4.12e-149	13	628	4	620
BAQ63458.1	1.46e-140	58	654	43	630
BAZ70078.1	2.83e-140	49	630	94	666

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	1.88e-102	76	619	22	717	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	4.42e-101	63	610	9	715	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	1.48e-74	58	629	5	769	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3ZG8_B	2.80e-68	177	591	3	433	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5FGZ_A	2.96e-65	56	593	122	671	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	2.82e-105	59	629	29	679	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q07806	7.31e-104	35	610	4	742	Penicillin-binding protein 1A OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mrcA PE=1 SV=2
O87579	2.28e-103	44	617	20	705	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
O86088	1.22e-102	47	617	23	705	Penicillin-binding protein 1A OS=Neisseria cinerea OX=483 GN=mrcA PE=3 SV=1
P0A0Z5	2.38e-102	47	617	23	705	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999919	0.000099	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
29	51