CAZyme Information: MGYG000000581_00544

Basic Information

• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; RF32; CAG-239; CAG-495
• CAZyme ID: MGYG000000581_00544
• CAZy Family: GH77
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
715	MGYG000000581_6|CGC1	81664.09	6.1169

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000581	1426046	MAG	Madagascar	Africa

• Gene Location: Start: 4437; End: 6584 Strand: +

Full Sequence      

MNQNLQGLAQKLGLADRFTDAGIMRREYTVSDETLKFFIKILGYNADDEAHIAASFSKLA
DNEWKLGFEPVCVVAADKVELRVVLPEDVAHEYFALRLSSHDDNQKQDVSFEVADDEQTA
VVKNKKLRRLTIRINTSLPIGYYDVRLGVAGKNYKTLLAVCPLRCYEAPAMANKLWGFAV
QLYSVKSQRNWGVGDFTDLCNLVRICSRAGAAVIGLNPLNTLMHNFPEDASPYLSISRLF
LNPIYIDVEEVPEFTAEDKAEVDSELPDLRASELIQYTKVYNLKMRMLERCYARFSAGLN
QERQAAFRAFCQEQGEDLHKLAVFQTVYDEMCHKVWGGWCAWPKELQNPDNPEVQKYAEE
HKEKVGFFKYLQFEAARQLNRAQNLVRELGLGIGFYRDLAVGVGKDSAELWSNPQLFIPK
VGAGAPPDAFFPTGQKWCLGAFNPFKLKENRYEAFLKILRANMKNAGALRIDHVMSLMRL
YLIPDKSEQGTYLYYNFADMLNLVALESHLNHCAVVGESIGNVPDGFLEILRERNVHSLS
VLWAERYDSGWGDFISPSGYPATAFTSVGTHDMAPLRMWWFGYDIELSRSLGLIATEEDK
QNAYHKRELDRWKLLFALDSNAVWPQDNLRKANYIYGEAYPEGIEEAVHRFVSRSASQVF
LAQLEDILHVEKLQNLPGTDRDKHPNWRRKLPVDLEKLENDIAYIRNIAAIKKER

Enzyme Prediction     

No EC number prediction in MGYG000000581_00544.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH77	180	712	1.8e-137	0.9878542510121457

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11052	malQ	0.0	1	715	2	689	4-alpha-glucanotransferase; Provisional
PRK14507	PRK14507	0.0	1	715	4	721	malto-oligosyltrehalose synthase.
pfam02446	Glyco_hydro_77	3.05e-144	180	694	1	460	4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan.
COG1640	MalQ	3.12e-139	165	715	1	516	4-alpha-glucanotransferase [Carbohydrate transport and metabolism].
PRK14510	PRK14510	8.70e-128	174	715	723	1218	bifunctional glycogen debranching protein GlgX/4-alpha-glucanotransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCO01675.1	6.57e-144	4	715	3	728
QCN94491.1	5.15e-143	4	715	3	728
QKS54043.1	1.48e-142	4	701	3	714
AIB11421.1	4.03e-142	4	715	3	728
QCO14157.1	1.13e-141	4	715	3	728

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3P_A	2.53e-119	136	701	102	673	AmylomaltaseMalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3P_B Amylomaltase MalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3Q_A Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_B Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_C Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12]
4S3R_A	5.20e-114	136	696	102	668	AmylomaltaseMalQ from Escherichia coli in complex with the pseudo-heptasaccharide acarviosine-glucose-acarbose [Escherichia coli K-12]
5B68_A	3.27e-77	5	714	7	706	Crystalstructure of apo amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum],5JJH_A Crystal structure of amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum]
2X1I_A	1.93e-23	191	641	20	450	glycosidehydrolase family 77 4-alpha-glucanotransferase from thermus brockianus [Thermus brockianus]
1TZ7_A	3.44e-20	174	694	22	486	Aquifexaeolicus amylomaltase [Aquifex aeolicus],1TZ7_B Aquifex aeolicus amylomaltase [Aquifex aeolicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P15977	4.73e-119	136	713	102	694	4-alpha-glucanotransferase OS=Escherichia coli (strain K12) OX=83333 GN=malQ PE=1 SV=2
P45176	8.57e-108	116	691	88	673	4-alpha-glucanotransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=malQ PE=3 SV=1
P9WK22	1.13e-81	4	688	7	684	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=malQ PE=3 SV=1
P65337	1.13e-81	4	688	7	684	4-alpha-glucanotransferase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=malQ PE=3 SV=1
P9WK23	1.13e-81	4	688	7	684	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=malQ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000037	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000581_00544.