CAZyme Information: MGYG000000584_00443

Basic Information

• Species: RC9 sp900544195
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; UBA932; RC9; RC9 sp900544195
• CAZyme ID: MGYG000000584_00443
• CAZy Family: GH127
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1257	MGYG000000584_6|CGC2	142801.35	5.2895

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000584	3056711	MAG	Madagascar	Africa

• Gene Location: Start: 13473; End: 17246 Strand: +

Full Sequence      

MKPLKTIVSLCMLWLAMAGYAKADATLNEPISFSSVTVEDSFWQPRIKRLSEVTVPFCLD
QCAKKTHRVDNFAIAAGVREGRFEGLFYDDSDLYKMIEGASYSLQNTPDKVLESQLDSII
ALIAGAQGKDGYINTYYTLKMPGKRWTDMDKHEMYCGGHLIEAGIAYYKATGKRVLLDVA
ERFAEYLVSEFGPGKKDWVPGHPEIELALIRLYHETSKKEYLDLAHFLLEERGRGKADWK
AYDYYIDKVPVAELSKIGGHAVRAMYLLTGMADYSATSGDTSYIAALDRLWDNVVKTRMY
LTGGIGSSRRNEGFTEDYDLPNKDAYCETCASVGMVMWNQRMNMLKGESRYVDVLERAMY
NGALAGISLSSDRFFYVNPLASDGDHHRKPWYGTACCPSQISRFLPSVGGYIYARSDDAL
WVNLYVSGKVETEISGVKTTFSQKTEYPWDGTVSMTVSPEKKTAFAVRLRIPGWCEEYNV
SVNGKKVRYVLDGGYVVLERKWRKGDRIVFEMNMPVKVVTSDPKVKANIGKRAIQRGPIV
YCMEQVDNAGFDAIRLTSDDVFQCVFEPETLGGVCTVTASSNGRSVRFVPYYAWDNRAPG
KMKVWVEYESNTIDKQSRLRPTAYQELPLGAVKAEGWLKEMLLRQSNGMTSRMDVLYPQV
MGDRNGWLGGDGDQWERGPYWIDGLLPLAYILDDQDLKDKAKPWIEWALASQREDGFFGP
DRDYPYESGLQRNNSHDWWPRIVVLKVLQQYWSATGDERVIDFMRRYFRYQLATLPETPL
GRWTFWAEYRACDNLNMVLWLYDVTKESWLIDLAGVLHAQGHDYVKMFLESDDLHRLSTI
HCVNLAQGIKEPVVYWRVDPDQKYLDAVDKAFSDIRQYNGFPNGMFGGDEALHGNNPTQG
SELCSAVELMYSLEEMAKVTGRLEYVDHLERIAFNALPSQITDDFTARQYFQQPNQVMVT
RCIHNFDINHGNTDLVFGLLTGYPCCTSNLHQGWPKFTRNLWLSSPDGGLAALAYSPSSV
RKSFSGVDVLIMEKTRYPMDGRIDFEVSVPSGGKVSFPLHLRVPSWSVRPSLTVNGEPVS
VTVGSMAVLDREWSDGDRVVLDLPMEVSLSEWYENSVAVERGPLVYALKMKEKWVKCDIG
EENSIYGNDYWEVLPESPWNYGIIDPAKSSLSDCFEVKVDEKLLDSEWYWNPESAPVSIM
VNAKRIPSWTLYNGMAGPQPYSRMYGPDTKDVPVERITLIPYGCTTLRISEFPVIKE

Enzyme Prediction     

No EC number prediction in MGYG000000584_00443.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	36	542	1.7e-207	0.9980916030534351
GH127	857	1127	3.4e-32	0.5229007633587787

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	2.43e-179	33	609	9	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	9.78e-175	35	542	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
pfam07944	Glyco_hydro_127	9.13e-55	680	1127	62	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	3.57e-25	694	1128	84	502	Uncharacterized conserved protein, DUF1680 family [Function unknown].
cd04791	LanC_SerThrkinase	1.61e-04	163	303	89	243	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABR45039.1	1.35e-255	18	608	20	615
AST55952.1	1.35e-255	18	608	20	615
QUT97168.1	1.35e-255	18	608	20	615
QKH99528.1	1.35e-255	18	608	20	615
QUT20768.1	1.35e-255	18	608	20	615

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	2.03e-166	29	616	8	633	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	1.11e-165	34	606	14	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	6.02e-160	34	606	14	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	2.72e-118	35	605	2	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	3.70e-118	35	605	2	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.49e-117	35	605	2	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000268	0.999086	0.000192	0.000145	0.000139	0.000130

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000584_00443.