CAZyme Information: MGYG000000587_00443

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UBA1381
• CAZyme ID: MGYG000000587_00443
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
565	MGYG000000587_50|CGC1	65120.87	4.5853

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000587	1865333	MAG	Madagascar	Africa

• Gene Location: Start: 4426; End: 6123 Strand: +

Full Sequence      

MYREEHPNPQFYRDSWKNLNGTWGFEFDFGCSGRDRKVYEQEKLEKFIEVPFCPESALSG
IEYKDFINGVWYMRSVEIPEDWKNSGRVFLHFGAVDYHAVVYVNKKEAGEHFGGYTSFKL
DITELLEEGKNTIAVFAEDDNRHGQQPCGKQAHFFASSGCDYTRTTGIWQTVWLEHTPEA
YIENFEFYPDVTNGKLSFRVYTVGCGDLTVNAAYEGKPMGDVTVHSEGVVCGEIQLAEKH
LWEIGCGRLYDVEMHFGDDCVKSYFGLRSVRMDGMKFLLNGKSVFQRLVLDQGFYPEGIY
TAPKEEDLKKDILLSLDAGFNGARLHEKVFEPRFLYYCDKLGYLAWGEFPNWGLDYSQPE
ILASFMSEWKEAVTRDFNHPSIIGWCPFNETWDYVGKRRQWDPLLSSVYYMTKAIDPTRP
CIDTSGNYHTVTDIFDVHDYEQDPEKFAAHFEKLSEGVVENSCNSRQTYTGGPFFVSEYG
GICLNADGTEGWGYGNAPKSLEEYIDRYDKLTTTLLDDPNMFAFCYTQLYDIEQEINGLY
TYDRTPKLDMSIIKKINTKKAAIED

Enzyme Prediction     

No EC number prediction in MGYG000000587_00443.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	11	556	7.5e-99	0.613031914893617

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	3.22e-42	17	437	13	466	beta-D-glucuronidase; Provisional
COG3250	LacZ	1.45e-38	1	527	1	533	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02837	Glyco_hydro_2_N	2.45e-17	17	178	2	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
PRK09525	lacZ	9.74e-16	72	434	124	499	beta-galactosidase.
PRK10340	ebgA	2.13e-15	48	419	99	470	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHQ60389.1	2.58e-256	3	564	6	570
ALS26546.1	5.11e-241	3	565	11	587
QOS82722.1	2.60e-234	3	565	6	580
ANY74929.1	3.95e-234	3	565	9	582
SMF87854.1	6.46e-233	18	565	24	582

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	3.55e-78	5	559	28	581	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
7VQM_A	1.48e-36	19	565	16	569	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]
3K4A_A	3.69e-36	17	555	15	588	Crystalstructure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12],3K4A_B Crystal structure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12]
4JHZ_A	1.22e-35	17	555	15	588	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12],4JHZ_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12]
3LPF_A	1.24e-35	17	555	15	588	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPF_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPG_A Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],3LPG_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],5CZK_A Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12],5CZK_B Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	3.04e-34	17	555	13	586	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
Q4FAT7	3.49e-27	17	428	40	487	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1
P06760	1.10e-26	17	555	39	620	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1
O97524	1.11e-26	17	480	39	541	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
O18835	1.98e-26	17	480	39	541	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000075	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000587_00443.