CAZyme Information: MGYG000000624_00295

Basic Information

• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Duodenibacillus
• CAZyme ID: MGYG000000624_00295
• CAZy Family: GH102
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
395	MGYG000000624_6|CGC1	44222.27	6.7151

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000624	2427641	MAG	Madagascar	Africa

• Gene Location: Start: 5161; End: 6348 Strand: +

Full Sequence      

MGKKLRIIKGLSALGLCALLAACGSVSQRQPDWMTDYPITSGIVRFKPVAFEEMPATERA
DWANALSAFRISCTAMSRKNLWQEACAQAQFVNEDEAYEFFRNAFDAYRVGYVEGEQFSE
TGFATGYYEPMLRGSLTRHDQFQYPIYGIPDDLIVVDLTELHPQLKGLRLRGKVVGRKLV
PYDTRSGIEKREDLKEANVLCWVDNAVEAFFLQIQGSGRILLEDGSFMRVGYGDQNGHPY
KAIGTWLIQNAGLQRSEMSMQRIKQWVNDNPKRTSELLNTNPSFVFFQKREGFADDDGPL
GAQEVPLTAGASVAVDRRYWPLGLPFVLDVKQENPDLAFTRPVIAQDTGGAIKGAVRFDY
FWGYGDLAGEQAGAQKSRVSAWVLVPKGKTPTNLR

Enzyme Prediction     

No EC number prediction in MGYG000000624_00295.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH102	132	288	5.8e-59	0.9936305732484076

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG2821	MltA	4.81e-103	55	388	36	367	Membrane-bound lytic murein transglycosylase [Cell wall/membrane/envelope biogenesis].
pfam03562	MltA	2.25e-98	58	288	2	231	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
cd14668	mlta_B	6.40e-79	132	288	1	157	Domain B insert of mltA_like lytic transglycosylases. Escherichia coli MltA is a membrane-bound lytic transglycosylase comprised of two domains separated by a large groove, where the peptidoglycan strand binds. Domain A is made up of an N-terminal and a C-terminal portion, which correspond to the 3D domain, named for 3 conserved aspartate residues. Domain B is inserted within the linear sequence of domain A. MltA is distinct from other bacterial lytic transglycosylases (LTs), which are similar to each other. Escherichia coli peptidoglycan lytic transglycosylase (LT) initiates cell wall recycling in response to damage, during bacterial fission, and cleaves peptidoglycan (PG) to create functional spaces in its wall. PG chains (also known as murein), the major components of the bacterial cell wall, are comprised of alternating beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), and lytic transglycosylases cleave this beta-1-4 bond. Typically, peptidoglycan lytic transglycosylases (LT) are exolytic, releasing Metabolite 1 (GlcNAc-anhMurNAc-L-Ala-D-Glu-m-Dap-D-Ala-D-Ala) from the ends of the PG strands. In contrast, MltE is endolytic , cleaving in the middle of PG strands, with further processing to Metabolite 1 accomplished by other LTs. In E. coli, there are six membrane-bound LTs: MltA-MltF and soluble Slt70. Slt35 is a soluble fragment cleaved from MltB. Bacterial LTs are classified in 4 families: Family 1 includes slt70 MltC-MltF, Family 2 includes MltA, Family 3 includes MltB, and Family 4 of bacteriophage origin. While most of the LT family members are similar in structure and sequence with a lysozyme-like fold, Family 2 (including mltA) is distinct.
smart00925	MltA	3.86e-71	131	288	1	153	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
PRK11162	mltA	3.97e-52	125	384	119	354	murein transglycosylase A; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF22454.1	1.70e-140	12	395	9	394
QDA55021.1	1.93e-131	6	394	15	394
ANU66434.1	1.41e-130	12	395	12	400
QQQ97583.1	1.41e-130	12	395	12	400
QQS89395.1	5.94e-118	38	391	40	393

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7ESJ_A	2.50e-89	38	388	16	361	ChainA, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii],7ESJ_B Chain B, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii]
2G5D_A	9.55e-76	39	391	35	418	Crystalstructure of MltA from Neisseria gonorrhoeae Monoclinic form [Neisseria gonorrhoeae FA 1090]
6QK4_B	7.17e-75	45	386	24	346	Lytictransglycosylase, LtgG, of Burkholderia pseudomallei. [Burkholderia pseudomallei]
2G6G_A	1.80e-72	61	391	57	418	Crystalstructure of MltA from Neisseria gonorrhoeae [Neisseria gonorrhoeae FA 1090]
2PNW_A	1.56e-45	84	387	62	364	Crystalstructure of membrane-bound lytic murein transglycosylase from Agrobacterium tumefaciens [Agrobacterium fabrum str. C58]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0A935	4.95e-31	125	384	119	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli (strain K12) OX=83333 GN=mltA PE=1 SV=1
P0A936	4.95e-31	125	384	119	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli O157:H7 OX=83334 GN=mltA PE=3 SV=1
Q8K9A7	1.58e-27	125	384	127	364	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=mltA PE=3 SV=1
P57531	6.73e-23	125	387	119	359	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=mltA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000065	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000624_00295.