CAZyme Information: MGYG000000625_00421

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-127
• CAZyme ID: MGYG000000625_00421
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
328	MGYG000000625_7|CGC1	36843.56	4.339

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000625	2232283	MAG	Madagascar	Africa

• Gene Location: Start: 14846; End: 15832 Strand: +

Full Sequence      

MNEKKRDYEINWPHVILVGAIAVVLVLIGLVIGINIGKSRRHKPALVGRDGYTWSLQPGA
EAQMIYANFWQFAQASYVLVGDIPNCQFYKIVKEVPRNDFQTDDFYMEDGDSTIMAYHNA
EGERISELAVDISSYQPDLDWDVLKQAGVTIAFIRVGYRGYGSEGKLVADDMFQTHIEAA
KAAGIKVGMYFYSQAVNYEEGVAEAQFALDLASDYTLDMPVVIDTEEVYADEARTADITV
DERTDGVVGFCETVKNAGYVPMIYSHRNWFVQKLDMTRLGGYKLWLAHYANQPDFPYLYA
GWQYTGSGTIAGIGQEIDLNVWLDGSVY

Enzyme Prediction     

No EC number prediction in MGYG000000625_00421.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	130	313	4.9e-44	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06414	GH25_LytC-like	2.90e-73	130	322	4	190	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
cd00599	GH25_muramidase	6.41e-36	130	322	3	186	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
pfam01183	Glyco_hydro_25	1.43e-32	130	313	1	180	Glycosyl hydrolases family 25.
cd06525	GH25_Lyc-like	2.74e-32	130	320	3	182	Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by the lyc gene. Lyc has a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
cd06524	GH25_YegX-like	9.62e-26	128	321	1	191	YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) catalytic domain that is similar in sequence to the CH-type (Chalaropsis-type) lysozymes of the GH25 family of endolysins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWT52701.1	3.31e-238	1	328	1	328
QNL99368.1	5.26e-201	3	328	4	329
CBK83179.1	6.12e-82	6	322	7	319
QYX28904.1	6.13e-66	90	324	55	286
QRO38911.1	1.88e-64	90	321	55	283

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAG_A	1.66e-08	123	321	12	205	TheStructure of a family 25 Glycosyl hydrolase from Bacillus anthracis. [Bacillus anthracis str. Ames]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P34020	4.76e-18	130	325	4	183	Autolytic lysozyme OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=lyc PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
12	34