CAZyme Information: MGYG000000626_00607

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_J
• CAZyme ID: MGYG000000626_00607
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
634	MGYG000000626_14|CGC2	73812.68	5.9902

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000626	2453643	MAG	Madagascar	Africa

• Gene Location: Start: 20675; End: 22579 Strand: -

Full Sequence      

MGNFTELDMYLFGQATHYDIYKKFGAHCMTVKRKQGVMFDLWAPGAKRVWVIGTFNDWNE
TKHEMERLEPTECGIYELFIPGVKLGDMYKYLIEGADGEMYYKADPYANEAELRPGTASV
VADIDHFVWTDEEWMESRAQKNVYREPMSILEVHPGSWMRHPGREDDGFYSYRELAKSLI
KYVKQMGYTHVELMGISEYPFDGSWGYQVTGYYAPTSRYGSPEDFAYLVDQLHKHKIGVI
LDWVPAHFPRDAHGLANFDGTCLYEYADPRMGEHPDWGTKIFNYGKNEVKNFLIGSALMW
IEHYHVDGLRVDAVASMLYLDYGKEDGQWVANKYGENKNLEAIEFFKHINTLITGRNQGA
VMIAEESTAWPMVTGKAEDGGLNFSLKWNMGWMHDFLDYMKLDPYFRKNNHYKMTFAMSY
NESERYILVLSHDEVVHLKCSMINKMPGDENSKFANLRLAYTYFMLHPGKKLLFMGQDFA
QLREWSEDRELDWYLLAEKPHQQIHDYMKELLHLYRKYPAMYELDHESNGFEWINADDKE
RSIYSFIRKSSDGKKCLICVLNFTPVARPDYRVGVPQAGSYRLILNSDEERFGGTGAEHP
KRFTAKKGEWDGQPYSVGYPLAPFGAVVFAFNYK

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	182	481	1.3e-150	0.9933554817275747
CBM48	23	111	2.2e-18	0.9078947368421053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	1	629	4	625	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	24	631	27	621	glycogen branching enzyme; Provisional
PRK14705	PRK14705	0.0	6	630	605	1221	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	5	630	7	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK05402	PRK05402	0.0	5	629	101	721	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VCV23983.1	0.0	1	631	1	632
CBL10287.1	0.0	1	631	1	632
CBL13518.1	0.0	1	631	1	632
AEN98279.1	0.0	1	631	1	633
CBK92321.1	0.0	1	631	1	652

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	1.65e-234	5	628	130	770	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	3.31e-234	5	628	130	770	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	4.69e-234	5	628	130	770	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	6.65e-234	5	628	130	770	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	9.41e-234	5	628	130	770	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	4.16e-298	5	634	8	635	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q8DLB8	6.21e-243	5	628	107	747	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q11EX1	8.37e-239	4	628	113	733	1,4-alpha-glucan branching enzyme GlgB OS=Chelativorans sp. (strain BNC1) OX=266779 GN=glgB PE=3 SV=1
Q7NL20	2.01e-238	5	629	104	726	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
P52981	3.48e-238	5	628	110	749	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000077	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000626_00607.