CAZyme Information: MGYG000000627_01768

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-632
• CAZyme ID: MGYG000000627_01768
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
734		85819.39	5.1407

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000627	2305272	MAG	Madagascar	Africa

• Gene Location: Start: 2107; End: 4311 Strand: +

Full Sequence      

MKTRKDVFAINWMEVDALVKARHDDPHHILGMHECIDDIYVNVFQPTAKQVTVINEKTKE
EYELEAKRVNGFFTVKIPKCKRFDYSIRMTREDGTEQSYIDPYMFEPVIDSIDISLFNEG
MHYEIYEKLGAHPMTVDGIQGVLFAVWAPNAERVSVVADFNHWDGRIHQMRRLPYSGIFE
LFIPGVGEHEIYKYEVRSRNGELKMKSDPYGNASELRPCNASIVTDLRKYEWKDDAWMQN
RDKVEASKAPMSIYEVYLGAWKRPTDGREYYNYRELAVKLAEYMKDMGYTHIELMPVMEH
PYDPSWGYQVTGYYAPTARYGSPEDFMYFVDYMHEQGISVIIDWVPAHFPKDENGLGRFD
GSCVYEHEDPRRGEHPHWGTYIFDYSKPEVKNFLIANALFWADKYHVDGIRMDAVASMLY
LDYGRDFGNWLPNMYGGNENLDAIDMLKQLNTVMKERYPSCIMIAEESTAWPMVSHPVAD
GGLGFDFKWNMGWMNDFLEYMKLDPLYRKYHHNELTFSMVYAYSEHFILVLSHDEVVHEK
GSMIAKMPGGYEDKFSNLRLAYGFMMTHPGKKLLFMGQEFAQFKEFNESDELDWGIFEFD
AHVCIRDYCRALNHLYQQEPALYALDDDPEGFEWINSIASNESLLVFARKTEKKEDTLVI
VCNFTPVEHVNYNIGVPYPGKYKEIFTSEEERFGGEGKKNKTLKQSKKVVCDERENSISI
YIAPLSISVFKFYK

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	282	582	3.3e-157	0.9966777408637874
CBM48	128	214	5.4e-18	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	113	732	11	627	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	23	731	516	1221	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	104	731	1	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14706	PRK14706	0.0	129	732	27	621	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	13	731	4	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM00753.1	0.0	1	731	1	730
QWT54329.1	0.0	13	731	1	718
QIB58408.1	0.0	10	734	9	734
QMW77842.1	0.0	10	734	9	734
CBK76766.1	0.0	10	730	9	740

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQZ_A	3.19e-256	14	731	29	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR5_A	6.41e-256	14	731	29	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	6.41e-256	14	731	29	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A	2.58e-255	14	731	29	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	3.65e-255	14	731	29	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8DLB8	1.80e-276	14	731	7	749	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
P52981	3.53e-262	8	731	3	751	1,4-alpha-glucan branching enzyme GlgB OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgB PE=3 SV=1
Q1AZ86	5.45e-262	15	726	7	713	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q2JT08	4.05e-261	8	732	3	753	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
Q608L5	8.23e-261	18	726	17	719	1,4-alpha-glucan branching enzyme GlgB OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000066	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000627_01768.