CAZyme Information: MGYG000000631_00741

Basic Information

• Species: Prevotella sp002299635
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp002299635
• CAZyme ID: MGYG000000631_00741
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
858	MGYG000000631_7|CGC1	97323.95	8.7365

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000631	2636006	MAG	Madagascar	Africa

• Gene Location: Start: 85763; End: 88339 Strand: +

Full Sequence      

MRKLKKAVQWAWRKIKAFWPWYKNLYKGRAWYTKTLIAFVSFLAFIFIYLGAVDINFLWL
FGKSPGYFSGILNPPSNQASEIYSADGKLIGKFFNENRTPVAYDQVNPSFFKALVDTEDE
RFYSRPRGMAVDVVGLFAVVKDVILHHDGRGASTISQQLAKNMFRVRSQYSTGLLGKIPG
LKILIVKSKEWIIATKLETVYNKRDIITMYANTVDFGNNAYGIKTASRTYFGTTPDSIST
QDCAVLVGMLKATSKYNPKTNPENSKRRRNVVLANMFHHGDLSREDCDSLQQLPITLHFK
VEENYDGQALYFREYVADYFKQNFRDDDGDPKYDLYSDGLKIYTTLDSRMQKYAEKAVHQ
QMKQVQQNFNNHWSIYRNQSKNWLRQAPWRDENGNEIPGFIDQIAQRQPFYKALLNRYPN
QPDSVNYYYRNWVHPVKLFDYDKGTIVKDMTSEDSIKYMVTFMHCAMLAMEPQTGAVKAW
VGDIDFKSWKYDKVTAERQPGSTFKLFVYTEAMNQGLTPCDKRRDEYISMDVYDKIQKKV
VTWTPTNANGSFSGDSIPLKSAFAKSINSVAVRLGQEMGINRIIATAEKMGIKSPLDDTP
SLALGSSDVQLLELTNAYCTVANNGMHHDPVIISKIVDKDGKTVYENNTEAEQVIPYKSA
FLMQQLLLGGTHEPGGTSQSLLAAEYAGGARDTDFGGKTGTSNNHSDAWFEGVSPNLVVG
AWVGGEYRSIHFRTGALGQGSRTALPIVGLFLHYVMDDPAFQKYHGRFKKPTADDGITSD
MYVCQSYQPRVVRDTTRVDSSRVISDQEVFLDENGEPIENQSSEEGNHSAESKSSKANET
QKSSKKDKSEKEINFNDL

Enzyme Prediction     

No EC number prediction in MGYG000000631_00741.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	86	276	7.4e-53	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	1.65e-139	97	756	1	529	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	1.34e-136	34	724	12	570	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	3.15e-128	36	779	13	735	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	4.15e-74	81	757	49	786	penicillin-binding protein 1a; Provisional
TIGR02071	PBP_1b	1.53e-66	67	724	113	656	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGB27916.1	0.0	1	823	1	815
VEH15546.1	0.0	3	818	2	805
BCS86586.1	0.0	1	822	1	810
QNT66403.1	0.0	1	818	1	808
ALO49868.1	0.0	1	858	1	829

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	5.27e-67	81	758	22	725	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	3.20e-60	81	747	22	707	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5U2G_A	1.53e-55	78	746	20	748	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3ZG8_B	7.43e-45	196	757	2	458	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5FGZ_A	1.74e-39	67	724	122	667	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	1.47e-72	74	747	41	659	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q9ZCE9	3.39e-69	74	781	45	743	Penicillin-binding protein 1A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=mrcA PE=3 SV=1
Q92G78	6.70e-69	74	747	45	708	Penicillin-binding protein 1A OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=mrcA PE=3 SV=1
Q4UK08	1.73e-68	74	810	45	767	Penicillin-binding protein 1A OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=mrcA PE=3 SV=1
Q68VU2	5.66e-68	74	811	45	774	Penicillin-binding protein 1A OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999991	0.000032	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
36	58