CAZyme Information: MGYG000000642_00581

Basic Information

• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Arcanobacterium_A
• CAZyme ID: MGYG000000642_00581
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1403		149028.07	4.9134

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000642	1644124	MAG	Madagascar	Africa

• Gene Location: Start: 141458; End: 145669 Strand: -

Full Sequence      

MKTTMASRFAAALLSAALGFSGGYALMHTQAAYADPVELTQPESNPTDFADTASESPEDI
FASADTTLQSWQSEKTQSMVNKPYLGALKPSGDYGTFGEGFISADTSDSTDAKMGLITFD
LSKYDKVPTSAKMKLTYIGYRGNPSDAATDSVNVMAVSTSSCTNNAASCPPASATWATRP
DFSSKDAVIAKSQKVTLKSGVYYTNDSLTIDPTKTTTLEIDVTDIVKAQFAAGNKTITFA
VQESEGAEMRFISSEGAVSFKGATSEMAPQLVVTPQVAPYTISVTQNPKLRYKLGEDFDP
SGIVVTKKDTATNATSVLSADQYTIDSSAFSKDNVGNYPIIIALVSDPTVTTRMTATVAG
TSGAADDGDISGDDVMWYAQPASQTSNGNVASGGTISGDNDTWQRHTLPIGNGKVGGTVW
GEIGRERITFNEETLWTGGPGTTSTYNGGNNGAKGRDGATLRALNKQLEAGAQTVNPGNL
TGGENPKEQGSYQNWGNIYIDYGFSANSDAKDFKRSLNLSRGKADVTFTRDNVTYTREFF
VSNPDNVMVVRLSASETEALNLNITFPSNTGFSKTGETTTVSGDTLTVKGALANNGLLYD
AKIKAVLDDGVGSVGAGTRGALKISGATGVTLYIAAATNYKQEYPNYRNGEDAGGLDARV
AGYVQSAANKGYAAVKNAHIADHSKLYKRVTIDLGQDSTAGEKSLATDALLNAYKAGTAS
TAQKRALEMLVYQYGRYVTIGSSRENSQLPSNLQGIWSSTADDNAHGATPWGSDFHMNVN
LQMNYWPTYSANLGELAKPLIDYAKALVEPGRVTAKTYAGASTDTGTPIGEGAGYMAHTE
NTAYGWTAPGHTFSWGWSPAAMPWLLQNVYEAYEFSGDKALLKDTIYPLLKEEANFYVNY
MLHKGGQKAADGSYRLTTGVAYSPEHGPLGTDGNTYESSLVWQLLNDSIEAANALGADAN
LVSNSSTCSVDNWAKDDNGTFTRTDANRSWSCALSLLKPIEVGSSGQIKEWYFEGDLGKK
TDGSPIGSYQKNHRHLSHMLGLFPGDLITADNSQYMDAAKVSLRNRGDDATGWGVGQRIN
AWARTGDGNHAYQLVEKQLKNAMYPNLFDAHPPFQIDGNFGNTSGINEMLLQSNATYTKG
DTQYANYMNLLPALPDAWSAKGSAQGLVARGNFVVDMKWKNGTISDLSVTSVLGKQATLK
FTGAGDAVFLDKTANKLVSVSTLDADHVTFPTEAGHTYTLIKIQEVTPQVPTLSTPKCAE
KAEVILPELIEGVEKYTQTIEDDKATVTAVLKAGYKLAPNAQGVWTFNVAPTPCPELPDS
SEHTPDNPQDNAADSGNPQQPSGGSSSGVQGLEPVAPADRPQLSSSGATIASVILGSFFL
LGSGIACYASASRIRRGRGAGSR

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	401	1204	1.1e-238	0.9695290858725761

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	6.45e-67	376	642	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
NF033679	DNRLRE_dom	4.45e-07	84	274	4	164	DNRLRE domain. The DNRLRE domain, with a length of about 160 amino acids, appears typically in large, repetitive surface proteins of bacteria and archaea, sometimes repeated several times. It occurs, notably, three times in the C-terminal region of the enzyme disaggregatase from the archaeal species Methanosarcina mazei, each time with the motif DNRLRE, for which the domain is named. Archaeal proteins within this family are described particularly well by the currently more narrowly defined Pfam model, PF06848. Note that the catalytic region of disaggregatase, in the N-terminal portion of the protein, is modeled by a different HMM, PF08480.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSY56848.1	0.0	64	1242	52	1229
QTB91571.1	0.0	64	1242	52	1229
AXM92304.1	0.0	58	1242	270	1471
QRI58870.1	0.0	58	1242	270	1471
AFL03772.1	0.0	58	1242	270	1471

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	0.0	367	1242	11	896	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	0.0	367	1242	11	896	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	0.0	367	1242	10	895	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
2RDY_A	7.41e-105	408	1240	18	789	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	2.14e-103	370	1200	17	742	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	2.03e-83	395	1191	55	804	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	4.75e-81	408	1186	39	763	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	2.27e-63	410	1189	46	786	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	2.44e-46	410	1192	35	708	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.011426	0.986811	0.000598	0.000629	0.000277	0.000232

TMHMM  Annotations     

start	end
1370	1392