CAZyme Information: MGYG000000645_00896

Basic Information

• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella
• CAZyme ID: MGYG000000645_00896
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1211		131381.66	6.8238

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000645	2128522	MAG	Madagascar	Africa

• Gene Location: Start: 17690; End: 21325 Strand: +

Full Sequence      

MKRCVARALAASFACVALLCVPLCAHAEDVAVDQSQEMIVDQSQETAGDQAASMPDDGLV
DADDSDDAAALDVEDSESPDALTGSAKASILGRSVVNINQGWSFSTNDTTLDGWGFPDGR
SSGTVDLPHSWEYAHPTRSYIPAFNKKTVTYSKTVDVSSYQGRRLYLKFYGVSKNAEVLV
DGKVVGSHVGGYSAFVVDITDAVQGKSKVGLTVNVTNIDTDSIPINVDYTQWAGIYRDVE
LIAMEDQHFSLMNAGSQGVFFDYNLQGGNANVAVRADISNHGDKGSVTLRSQILDANGNV
VSEAEQPCSLAANKDFTEFNIKQKVANVHRWNGVADPYLYTAVVQICGTDGSVLDEVSQS
IGFRTFKISKGSAYLNGVKIQIHGVGYHQDRQGCGNAVTADQISSDIDQMLDMGVNFVRA
SHYPHDQAFYELADQKGILVYNEIPYYMIYSKAKSYGDSINTQLVEMIRQNYNHPCVVMD
GIQNEVVYNPSFKNYGPDFDVTAGEIIAFNKRLAALAKAEDPSRLVVQATIDASSHAQNS
KQWSGNIELTGLNLYVGFKSAVSSAGNEGRRELAKLLNAKLDSYKSIYGVDSLMISEYGA
GANVQQHCEVDDGFSWDGKDSSNSHYEEYQSFIHEVYWDTIQKRSDLPVTSVWNMFDFSC
YRNEGGVPRTNTKGLLCYDHETRKDAYYFYKANWNTDDPLVYLTSKRFLVRNRSTEDFKV
YSNCDKVKLYVNGSSVGYGTKQQSGVFVWKSVKLKKGVKNSVKAVGERGGATYEDSVDGI
TVPDHNVSYRAHVQNIGWQQDRQNGSIAGTVGKSLRIESLAASLDDPNSSIKINGHVQGI
GWQGWKTGSCGTTGKSKRLEAIRVRLTGSLSNDYDIWYRVHSSHVGWLGWAKNGAPAGTT
GKGYAAEAVQMVLVKKGDPAPGDTANSFIGKDRIVTYKAHVATIGWQSSVSDGATAGTTG
RSLSMEALSAAVSSDPDALKLNAHVQNIGWQGWQTGNCGTTGKSLRMEAIKIKLTGELRE
KYDVYYRAHVANFGWLGWAKNGEAAGTQGYAYGMQAVEICLVEKGGKAPGKTGNSFRQPL
VTYSAHVAHIGWMSAAKDGKTAGTTGRGLSMEALRLSLGSSVSGGIEAKAHAAGTGWESG
WSTTVGTTGESRAIEAVRIRLTGDAAEKYDVMYRAHSAKVGWSAWVKNGKKAGTTGYSLP
MQAIEIKLVKK

Enzyme Prediction     

No EC number prediction in MGYG000000645_00896.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	90	731	1.7e-90	0.660904255319149

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	2.23e-47	93	696	10	594	beta-D-glucuronidase; Provisional
COG3250	LacZ	1.38e-44	91	488	8	409	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	6.62e-34	140	526	103	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.27e-17	234	526	208	485	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	3.70e-17	366	667	1	274	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAQ97096.1	3.93e-299	81	811	57	791
AXR42332.1	3.55e-295	77	780	68	773
AMC08061.1	8.72e-178	153	798	3	645
AIQ56230.1	6.65e-111	93	797	2	671
QFK71524.1	1.65e-106	93	789	2	662

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5Z1A_A	3.89e-89	87	757	17	652	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]
3CMG_A	3.92e-89	125	757	28	633	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
3FN9_A	1.34e-88	91	778	3	662	Crystalstructure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_B Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_C Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_D Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343]
6MVG_A	8.03e-87	93	737	25	620	Crystalstructure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_B Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_C Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus]
6MVH_A	8.26e-81	93	737	25	620	Crystalstructure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_B Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_C Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_D Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	3.78e-60	83	767	41	705	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P26257	4.67e-32	93	524	2	411	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
P77989	5.07e-31	91	483	1	385	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
A7LXS9	1.07e-28	85	787	34	751	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
A1A3T0	2.54e-28	125	485	102	483	Beta-galactosidase BgaC OS=Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) OX=367928 GN=bgaC PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001203	0.904382	0.093465	0.000345	0.000326	0.000282

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000645_00896.