CAZyme Information: MGYG000000657_03980

Basic Information

• Species: Pseudomonas_E bubulae
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas_E; Pseudomonas_E bubulae
• CAZyme ID: MGYG000000657_03980
• CAZy Family: AA3
• CAZyme Description: Oxygen-dependent choline dehydrogenase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
567		62766.73	5.9669

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000657	4450077	MAG	Germany	Europe

• Gene Location: Start: 1009; End: 2712 Strand: -

Full Sequence      

MSQEFDYIIIGAGSAGNTLATRLTEDQGVTVLLLEAGGPDYRFDFRTQMPAALAFPLQGR
RYNWAFETDPEPHMDNRRMECGRGKGLGGSSLINGMCYIRGNAMDYDGWAKYPGLEDWSY
LDCLPYFRKAETRDIGPNDYHGGDGPVSVTTPKAGNNVLFHAMVEAGVQAGYPRTEDLNG
YQQEGFGPMDRTVTPNGRRASTARGYLDTAKQRSTLKIVTHALTDKILFEGKRAVGVSYM
VGDSDTRIQAKARKEVLLCSGAIGSPTVLQRSGVGPAELLNSLDIPVVHDLPGVGQNLQD
HLELYLQYACTQPVSLYPSLLWYNQPAIGAEWLFLGKGIGASNQFEAGGFIRTREEFEWP
NIQYHFLPVAINYNGSNGVKEHGFQAHMGSMRSPSRGRINVKSKDPREYPSILFNYMASE
QDWQEFRDGIRLTREIMQQPALDAFRGREISPGIDKQTDEELDTFIREHAETAFHPSCSC
KMGTDEMAVVDGEGRVHGMQGLRVVDASIMPLITTGNLNAPTIMIAEKIADKIRGRQPEP
RSTADYYVAGDAPVRGKPLREVSRTAQ

Enzyme Prediction     

No EC number prediction in MGYG000000657_03980.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA3	3	534	4.4e-160	0.9947183098591549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG2303	BetA	0.0	1	538	4	538	Choline dehydrogenase or related flavoprotein [Lipid transport and metabolism, General function prediction only].
PRK02106	PRK02106	0.0	1	562	2	560	choline dehydrogenase; Validated
TIGR01810	betA	0.0	6	538	1	531	choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli, Staphylococcus xylosus, and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified. [Cellular processes, Adaptations to atypical conditions]
pfam00732	GMC_oxred_N	1.09e-62	75	303	15	218	GMC oxidoreductase. This family of proteins bind FAD as a cofactor.
pfam05199	GMC_oxred_C	6.14e-43	395	529	3	143	GMC oxidoreductase. This domain found associated with pfam00732.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAB3230510.1	1.15e-177	2	548	22	570
ANI26486.1	5.25e-177	2	536	68	604
AWP09430.1	5.56e-177	2	536	17	553
CAG5086058.1	8.76e-155	4	549	14	579
CAG5089487.1	8.46e-154	3	538	15	563

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6ZH7_A	3.13e-83	1	536	4	563	ChainA, Fatty acid photodecarboxylase, chloroplastic [Chlorella variabilis],6ZH7_B Chain B, Fatty acid photodecarboxylase, chloroplastic [Chlorella variabilis]
6YRU_AAA	6.14e-83	3	536	6	563	ChainAAA, Fatty acid Photodecarboxylase [Chlorella variabilis],6YRV_AAA Chain AAA, Fatty acid Photodecarboxylase [Chlorella variabilis],6YRX_AAA Chain AAA, Fatty acid Photodecarboxylase [Chlorella variabilis],6YRZ_AAA Chain AAA, Fatty acid photodecarboxylase, chloroplastic [Chlorella variabilis]
6YS1_AAA	8.61e-83	3	536	6	563	ChainAAA, Fatty acid Photodecarboxylase [Chlorella variabilis]
5NCC_A	9.05e-83	3	536	22	579	Structureof Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_B Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_C Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_D Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_E Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis],5NCC_F Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid [Chlorella variabilis]
6YS2_AAA	1.21e-82	3	536	6	563	ChainAAA, Fatty acid Photodecarboxylase [Chlorella variabilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q3BXK8	0.0	1	556	1	556	Oxygen-dependent choline dehydrogenase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) OX=316273 GN=betA PE=3 SV=1
Q4K4K7	0.0	1	567	1	567	Oxygen-dependent choline dehydrogenase OS=Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) OX=220664 GN=betA PE=3 SV=1
Q48CM7	0.0	3	566	5	568	Oxygen-dependent choline dehydrogenase OS=Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) OX=264730 GN=betA PE=3 SV=1
Q3K5H3	0.0	1	567	1	567	Oxygen-dependent choline dehydrogenase OS=Pseudomonas fluorescens (strain Pf0-1) OX=205922 GN=betA PE=3 SV=1
Q4ZM63	0.0	3	566	5	568	Oxygen-dependent choline dehydrogenase OS=Pseudomonas syringae pv. syringae (strain B728a) OX=205918 GN=betA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000055	0.000006	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000657_03980.