CAZyme Information: MGYG000000661_00102

Basic Information

• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; CAG-302
• CAZyme ID: MGYG000000661_00102
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
937		104868.76	5.0269

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000661	1287314	MAG	Kazakhstan	Asia

• Gene Location: Start: 38397; End: 41210 Strand: +

Full Sequence      

MSSRDRKKGITLKKEKEKKTSKKRTNKNTEKRKRNEKEKKGIGKKILTIFLGLAIFFVLL
IAIFMIYIVISTGNFDPNALVNQDQTVMYDKDGEVFATLGAQKRESVTYDELPQVLIDAI
VATEDSRYYQHNGVDMARFLKASIQQLLGQDGGGASTLTMQVVKNNLTDTSRSIIRKFKD
VYLSVFFMEKKYTKEEILEFYVNDSCLGGNIYGVEEASKYYFGKTVSELSLPEAALIAGM
YQAPNRYNPYKDPEAAAKRRSTVLSLMVRHGYITEEEEAMANAVDITSMLVGVGEESDYQ
GYIDTVIEEIENKTGYNPAQVPMKIYTALDRDIQDGINKVLSGEAHSWADDYVQAGIAVT
NVNDGTIVAIGAGRNREGERTFNYATQAYRQPGSTAKPLFDYGPGFEYNNFSTYTLFNDE
KWTYTNGPEVGNWDGAYEGLITLRRALSYSRNVPALKAFQQVDKKNIVNFVNGLGLNIAY
STKDENYKKYDNGADNLINEAYSIGGMSYGVTPLEMAEAYACFANGGYHIESHAVIKIEY
RNGGKVEEFTESKEKVMSDSTAYLMNNVLQYAVEAGFNGGARVYGSTVAAKTGTSNLPDS
IIEKYGLPGGAVNDLWTVAYTPEYSIALWYGYEEVTSEHYLSGASAPKDAVMQSIMKYIP
KTTKKWEMPSSVVAVTVEKETWPAELPSEYTPDNMKMTEYFKKGTQPTEVSKRYDKLDPI
TNLQSSKTSSGYKLTWNWKVPDILDETYLSQYFSNSVYGNQSAQYLKNRLDYNKNTLGGI
GFGIYIKDSSGVLERIAFTEETEYVYMPTTSGNIEIVVKAEYKNFKNNASDETSIDIKSD
GSSTPIEDDKLKVTLNGEESFSVVKGSYSEAGIKATYNGKDVTDEVTITYQINNTTFTAS
SELVNNINSLEKGQYEITYTITYNKEKQTLKRTITII

Enzyme Prediction     

No EC number prediction in MGYG000000661_00102.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	92	268	4.1e-60	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	2.91e-154	103	659	1	528	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	4.23e-153	47	681	17	622	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	5.76e-107	47	716	9	773	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	4.82e-73	101	681	144	704	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam00912	Transgly	6.17e-73	92	268	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
APH04640.1	2.58e-158	39	738	28	715
AYA76804.1	1.24e-157	40	739	31	724
QJS19460.1	5.76e-157	64	825	42	812
AIE60509.1	2.18e-154	5	738	10	720
QNG61477.1	4.67e-154	38	746	30	723

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3DWK_A	1.61e-86	88	635	10	560	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
2OLU_A	1.57e-82	88	635	19	569	StructuralInsight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Apoenzyme [Staphylococcus aureus],2OLV_A Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus],2OLV_B Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus]
3ZG8_B	1.80e-67	188	681	3	478	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3ZG7_B	1.65e-62	188	681	3	478	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
3UDF_A	5.03e-53	88	637	23	693	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39793	1.53e-144	37	738	28	729	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
Q00573	2.01e-100	66	632	33	600	Penicillin-binding protein 1A (Fragment) OS=Streptococcus oralis OX=1303 GN=ponA PE=3 SV=1
Q04707	3.20e-96	67	689	34	663	Penicillin-binding protein 1A OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=ponA PE=1 SV=2
Q8DR59	8.60e-96	67	689	34	663	Penicillin-binding protein 1A OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=pbpA PE=1 SV=1
P38050	1.11e-79	76	691	39	630	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999852	0.000171	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
47	69