CAZyme Information: MGYG000000683_00580

Basic Information

• Species: CAG-977 sp000434295
• Lineage: Bacteria; Proteobacteria; Alphaproteobacteria; RF32; CAG-977; CAG-977; CAG-977 sp000434295
• CAZyme ID: MGYG000000683_00580
• CAZy Family: GT2
• CAZyme Description: Glucans biosynthesis glucosyltransferase H

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
681		77591.07	8.3566

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000683	2086024	MAG	Kazakhstan	Asia

• Gene Location: Start: 39053; End: 41098 Strand: -

Full Sequence      

MKEFVPISKRRVFLHRLYVFGLSIISTLFATLKWITVIPSDSYLYTKLLMVLLFALTFGW
IALFFWSSLFGFWGLLRPTKPIGLKWADDEVSLTGKTALLMPVYNEEPVKVFADLLAMAQ
ELEETGQANHFDIFVLSDTTNPKYWVKEEKLWLETQLRMPENIHLYYRRRVKNTARKSGN
IEDFCNKWGSLYDFMIVLDADSLLSGKTLLKMSQLMEKNPDTGIIQAPPLCINRHSLFAR
IQQFAGKVYGGVIAAGSAYWQDGESNYWGHNAIIRVKAFMECCKLPVLTGKAPFGGHILS
HDFVEASLICRGGWRTWLLPELSGSYEECPPSMIDFAVRDRRWCQGNLQHIKVLLSKGLN
PISRIHFLIGIMSYVSSPIWLCFMLVGIAAALGRAFIPPDYFPVGYSLFPTWPIFDKFGT
IALFLLSMFMLFFPKFLGLAVYILQNRSKDIDGVFSAIKSVFAEIIFSILSAPVMMMFQS
KFVFDILLGRAVGWNTQNRDETGTPWKFAVKIHFWHTVLGILAAVVVWKYVNVLFWWTLP
VTLGLALSIPLSVLSSRESVGMWAKKHKYFVIPEEINVPSILEKAQKNLKELEPYAVFER
GVIDVICDSDLNALHLLMLPVNGPAEPAEEKTLKQGRIKLENFINYGELPDFSKEEETAL
LYSPDFLEASRLSYLLSLRNG

Enzyme Prediction     

No EC number prediction in MGYG000000683_00580.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG2943	MdoH	0.0	14	639	63	692	Membrane glycosyltransferase [Cell wall/membrane/envelope biogenesis, Carbohydrate transport and metabolism].
PRK05454	PRK05454	0.0	10	570	36	599	glucans biosynthesis glucosyltransferase MdoH.
cd04191	Glucan_BSP_MdoH	4.80e-150	97	350	1	254	Glucan_BSP_MdoH catalyzes the elongation of beta-1,2 polyglucose chains of glucan. Periplasmic Glucan Biosynthesis protein MdoH is a glucosyltransferase that catalyzes the elongation of beta-1,2 polyglucose chains of glucan, requiring a beta-glucoside as a primer and UDP-glucose as a substrate. Glucans are composed of 5 to 10 units of glucose forming a highly branched structure, where beta-1,2-linked glucose constitutes a linear backbone to which branches are attached by beta-1,6 linkages. In Escherichia coli, glucans are located in the periplasmic space, functioning as regulator of osmolarity. It is synthesized at a maximum when cells are grown in a medium with low osmolarity. It has been shown to span the cytoplasmic membrane.
cd06421	CESA_CelA_like	6.95e-17	102	351	8	234	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.
cd06423	CESA_like	8.31e-15	99	281	1	168	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRO00644.1	6.58e-199	14	674	13	670
ATB33264.1	2.44e-191	25	674	24	670
QSQ18747.1	6.91e-191	16	671	15	664
QPM81905.1	7.87e-191	14	671	13	668
QDE96388.1	7.87e-191	14	671	13	668

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0HJ63	7.80e-165	16	669	57	710	Glucans biosynthesis glucosyltransferase H OS=Shewanella sp. (strain MR-4) OX=60480 GN=opgH PE=3 SV=1
Q0HUS0	2.20e-164	16	669	57	710	Glucans biosynthesis glucosyltransferase H OS=Shewanella sp. (strain MR-7) OX=60481 GN=opgH PE=3 SV=1
A0KWF0	4.38e-164	16	669	57	710	Glucans biosynthesis glucosyltransferase H OS=Shewanella sp. (strain ANA-3) OX=94122 GN=opgH PE=3 SV=1
Q9KSG9	1.15e-162	16	641	56	679	Glucans biosynthesis glucosyltransferase H OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=opgH PE=3 SV=1
A5F1Q0	1.15e-162	16	641	56	679	Glucans biosynthesis glucosyltransferase H OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=opgH PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000031	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
17	39
54	76
376	398
418	440
453	475
512	531
533	555