CAZyme Information: MGYG000000701_00328

Basic Information

• Species: Prevotella sp900552675
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp900552675
• CAZyme ID: MGYG000000701_00328
• CAZy Family: GH9
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
835		94551.05	7.1548

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000701	3061545	MAG	Kazakhstan	Asia

• Gene Location: Start: 61098; End: 63605 Strand: +

Full Sequence      

MLWALLAFNALMMKGEGWVRINQLGYLPQATKVAVLMSDQPTTVDSFQLINAFTGKVAYT
SNQVQAMGAMGNMKATFRLRFSAFTQPGTYYIKVGQSRSEIFPINGQVYKGTSDFVLNYM
RQQRCGWNPYIQDSCHQKDARIKYHPTKEGQHIDVRGGWHDAADLLQYTPTSANAIYQML
FAYQQNPSAFTDHYQANGMPGANGIPDIIDEIYWGLDWLDRMNPAKGELYNQIADDRDHV
AMKLPNYDPADYGWGKNQDRPVYFVDGKPQQRGKFMNATMGAASTAGKFASDFALGSCVL
KPFYPEFAAKIGAKAADAYQVGVDKPGNTQTVSVVSPYIYEEDNWVDDMELGAMELYRKT
GDRQYLLQAVEYGRREPVTPWMGADSARHYQWYPFMNMGHYQLAKHGTDSRLRNEFVRNL
RAGIDRVWQRAQGHPFLWGVPGIWCSNNLTVAMLTQCILYRQLTNDRTYEEMEGALRDWL
LGCNPWGTSMIVELPMGGVYPTQPHSGYVYLNKGNTTGGLVDGPVYATIFKGLRGVNLTN
GMRANEARNYELFQPGDVVYHDNMSDYSSNEPTMDGTASLTFPLSVYEMEGREQTGVADN
ANVYEQAGAIIKGDPTKKRICLVFTADDKADGANTIISTLKKEKVKGGFFFTGKFYEMYP
DIVRQLVADGHYVGSHSYGHLLYAPWEKRDSMLVSKQQFVDDMLKSYNTMATFGISKEKA
PYFIPPYEYYNSTVNGWAHEMGLQIINFTPGTGTNADYTTPDMGERYKSSAQLYKRLLDF
ERQHTLNGHFLMIHFGTHPSRTDKFYTMLPKIIKTLQRRGYTFVSVEEMLGLNLK

Enzyme Prediction     

No EC number prediction in MGYG000000701_00328.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH9	109	582	2.3e-71	0.9880382775119617
CE4	617	746	7.2e-19	0.9153846153846154

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00759	Glyco_hydro_9	4.13e-58	117	584	7	374	Glycosyl hydrolase family 9.
cd10917	CE4_NodB_like_6s_7s	2.46e-30	618	817	1	171	Catalytic NodB homology domain of rhizobial NodB-like proteins. This family belongs to the large and functionally diverse carbohydrate esterase 4 (CE4) superfamily, whose members show strong sequence similarity with some variability due to their distinct carbohydrate substrates. It includes many rhizobial NodB chitooligosaccharide N-deacetylase (EC 3.5.1.-)-like proteins, mainly from bacteria and eukaryotes, such as chitin deacetylases (EC 3.5.1.41), bacterial peptidoglycan N-acetylglucosamine deacetylases (EC 3.5.1.-), and acetylxylan esterases (EC 3.1.1.72), which catalyze the N- or O-deacetylation of substrates such as acetylated chitin, peptidoglycan, and acetylated xylan. All members of this family contain a catalytic NodB homology domain with the same overall topology and a deformed (beta/alpha)8 barrel fold with 6- or 7 strands. Their catalytic activity is dependent on the presence of a divalent cation, preferably cobalt or zinc, and they employ a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. Several family members show diversity both in metal ion specificities and in the residues that coordinate the metal.
pfam02927	CelD_N	3.18e-28	18	99	2	83	Cellulase N-terminal ig-like domain.
cd02850	E_set_Cellulase_N	5.40e-26	18	104	1	86	N-terminal Early set domain associated with the catalytic domain of cellulase. E or "early" set domains are associated with the catalytic domain of cellulases at the N-terminal end. Cellulases are O-glycosyl hydrolases (GHs) that hydrolyze beta 1-4 glucosidic bonds in cellulose. They are usually categorized into either exoglucanases, which sequentially release terminal sugar units from the cellulose chain, or endoglucanases, which also attack the chain internally. The N-terminal domain of cellulase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.
cd10950	CE4_BsYlxY_like	3.54e-25	613	830	1	188	Putative catalytic NodB homology domain of uncharacterized protein YlxY from Bacillus subtilis and its bacterial homologs. The Bacillus subtilis genome contains six polysaccharide deacetylase gene homologs: pdaA, pdaB (previously known as ybaN), yheN, yjeA, yxkH and ylxY. This family is represented by Bacillus subtilis putative polysaccharide deacetylase BsYlxY, encoded by the ylxY gene, which is a member of the carbohydrate esterase 4 (CE4) superfamily. Although its biological function still remains unknown, BsYlxY shows high sequence homology to the catalytic domain of Bacillus subtilis pdaB gene encoding a putative polysaccharide deacetylase (BsPdaB), which is essential for the maintenance of spores after the late stage of sporulation and is highly conserved in spore-forming bacteria. However, disruption of the ylxY gene in B. subtilis did not cause any sporulation defect. Moreover, the Asp residue in the classical His-His-Asp zinc-binding motif of CE4 esterases is mutated to a Val residue in this family. Other catalytically relevant residues of CE4 esterases are also not conserved, which suggest that members of this family may be inactive.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT35459.1	0.0	1	832	7	836
QUT98069.1	0.0	1	832	7	836
QPH59586.1	0.0	1	832	7	836
QUT66756.1	0.0	1	832	7	836
QQA29127.1	0.0	1	832	7	836

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3X17_A	3.39e-36	19	582	18	551	Crystalstructure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium],3X17_B Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium]
5U2O_A	3.54e-29	27	585	2	537	Crystalstructure of Zn-binding triple mutant of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
5U0H_A	8.11e-26	27	585	2	537	Crystalstructure of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
6DHT_A	1.91e-19	19	505	18	497	Bacteroidesovatus GH9 Bacova_02649 [Bacteroides ovatus ATCC 8483]
7FBW_A	3.61e-14	618	830	117	300	ChainA, Predicted xylanase/chitin deacetylase [Caldanaerobacter subterraneus subsp. tengcongensis MB4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P23658	2.59e-22	19	585	4	543	Cellodextrinase OS=Butyrivibrio fibrisolvens OX=831 GN=ced1 PE=1 SV=1
Q05156	2.74e-21	19	580	185	735	Cellulase 1 OS=Streptomyces reticuli OX=1926 GN=cel1 PE=1 SV=1
A7LXT3	1.11e-18	19	505	32	511	Xyloglucan-specific endo-beta-1,4-glucanase BoGH9A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02649 PE=1 SV=1
P14090	3.75e-18	19	506	341	832	Endoglucanase C OS=Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) OX=590998 GN=cenC PE=1 SV=2
P0C2S1	3.69e-14	19	365	214	580	Cellulose 1,4-beta-cellobiosidase OS=Acetivibrio thermocellus OX=1515 GN=celK PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.673412	0.324387	0.001708	0.000171	0.000143	0.000186

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000701_00328.