CAZyme Information: MGYG000000708_00246

Basic Information

• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Succinivibrionaceae; Succinivibrio
• CAZyme ID: MGYG000000708_00246
• CAZy Family: GH77
• CAZyme Description: 4-alpha-glucanotransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
734		83687.4	6.0745

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000708	1500808	MAG	Kazakhstan	Asia

• Gene Location: Start: 7623; End: 9827 Strand: -

Full Sequence      

MEEKAIEALADEAGIARSYKDVNNREVIISTKARAETLKLMGYALDGSDASQIEERKVRL
CQNMLESVSVLNDKDFPFIYIRTRADIRENAVITWTFELENGERITRAEPIEEIEIARFY
EVRGIEYDVRRFIIPKDVTLPYGYHHFSCVVTDGYRIFNSRRMSLIRVPMQTYMPSEFQQ
GRKEWGVSVQLYALRSEKNWGIGDFEDLKDLLVKIAASGGQYVGLNPLHAGYPASPDPSN
CSPYSPSSRLWLNIAYISVPSVPEYVNCREACAKVASSEFQKELKLLREREYVDWHAVIE
LKLEVLKIIFRYMHVDDRRSIRGRKFLSFMEQGGAELLDMATYGAIQEELFSKGLDASGW
QAFPRELQHADSPFVEVWRNEHSSKVRFYCYLQFLANEQLEEAYAQADKSGMSLGIYRDL
AVGVSAGSCDVWSDHHRVYRVKASVGAPPDPLGPQGQVWGLVPMDPVKLKECAYEPMIRL
YRSNMRSCGALRIDHAAGLNRLWWVRDGQKASDGAYVASNMHDLLGIVALESVRNHCLII
AEDLGTIPQELRDALKECGAFSYKIFLYERAGDGGFIAPSDYPEYALATLTTHDMPTLKG
WWECRDLTDGKKMGIYSENEKEQLMADRALAKERMFDSMRYFGTLDKSITGKAAYTPFSQ
ELALSMQRHFCTTACLLCSSQLEDWIGVEKPVNVPGTMREYPNWRRKLTRDLNDIFDDPK
VKGITKAMNDARRA

Enzyme Prediction     

EC	2.4.1.25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH77	189	729	3.3e-139	0.9878542510121457

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11052	malQ	0.0	1	734	1	691	4-alpha-glucanotransferase; Provisional
PRK14507	PRK14507	0.0	1	732	3	721	malto-oligosyltrehalose synthase.
COG1640	MalQ	8.63e-159	173	734	2	518	4-alpha-glucanotransferase [Carbohydrate transport and metabolism].
pfam02446	Glyco_hydro_77	1.19e-148	189	711	1	460	4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan.
PRK14510	PRK14510	4.69e-133	185	732	725	1218	bifunctional glycogen debranching protein GlgX/4-alpha-glucanotransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALP43607.1	8.62e-207	6	732	5	724
QCG49066.1	8.62e-207	6	732	5	724
AUY10561.1	1.78e-203	6	732	5	724
AVP91870.1	2.52e-203	6	732	5	724
AXB04398.1	2.52e-203	6	732	5	724

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3P_A	8.13e-156	1	729	1	684	AmylomaltaseMalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3P_B Amylomaltase MalQ from Escherichia coli, apo structure [Escherichia coli K-12],4S3Q_A Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_B Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12],4S3Q_C Amylomaltase MalQ from Escherichia coli in complex with maltose [Escherichia coli K-12]
4S3R_A	5.45e-150	130	729	96	684	AmylomaltaseMalQ from Escherichia coli in complex with the pseudo-heptasaccharide acarviosine-glucose-acarbose [Escherichia coli K-12]
5B68_A	1.83e-79	9	704	10	673	Crystalstructure of apo amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum],5JJH_A Crystal structure of amylomaltase from Corynebacterium glutamicum [Corynebacterium glutamicum]
1TZ7_A	7.98e-18	186	632	25	443	Aquifexaeolicus amylomaltase [Aquifex aeolicus],1TZ7_B Aquifex aeolicus amylomaltase [Aquifex aeolicus]
5CPQ_A	9.33e-13	337	616	206	483	Disproportionatingenzyme 1 from Arabidopsis - apo form [Arabidopsis thaliana],5CPQ_B Disproportionating enzyme 1 from Arabidopsis - apo form [Arabidopsis thaliana],5CPS_A Disproportionating enzyme 1 from Arabidopsis - maltotriose soak [Arabidopsis thaliana],5CPS_B Disproportionating enzyme 1 from Arabidopsis - maltotriose soak [Arabidopsis thaliana],5CPT_A Disproportionating enzyme 1 from Arabidopsis - beta cyclodextrin soak [Arabidopsis thaliana],5CPT_B Disproportionating enzyme 1 from Arabidopsis - beta cyclodextrin soak [Arabidopsis thaliana],5CQ1_A Disproportionating enzyme 1 from Arabidopsis - cycloamylose soak [Arabidopsis thaliana],5CQ1_B Disproportionating enzyme 1 from Arabidopsis - cycloamylose soak [Arabidopsis thaliana],5CSU_A Disproportionating enzyme 1 from Arabidopsis - acarviostatin soak [Arabidopsis thaliana],5CSU_B Disproportionating enzyme 1 from Arabidopsis - acarviostatin soak [Arabidopsis thaliana],5CSY_A Disproportionating enzyme 1 from Arabidopsis - acarbose soak [Arabidopsis thaliana],5CSY_B Disproportionating enzyme 1 from Arabidopsis - acarbose soak [Arabidopsis thaliana]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P15977	1.34e-156	1	734	1	689	4-alpha-glucanotransferase OS=Escherichia coli (strain K12) OX=83333 GN=malQ PE=1 SV=2
P45176	4.73e-131	140	732	110	697	4-alpha-glucanotransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=malQ PE=3 SV=1
P9WK22	2.80e-91	9	731	11	716	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=malQ PE=3 SV=1
P65337	2.80e-91	9	731	11	716	4-alpha-glucanotransferase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=malQ PE=3 SV=1
P9WK23	2.80e-91	9	731	11	716	4-alpha-glucanotransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=malQ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000708_00246.