CAZyme Information: MGYG000000709_00958

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; UBA738
• CAZyme ID: MGYG000000709_00958
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 4

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
560		62610.87	4.1148

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000709	1364323	MAG	Kazakhstan	Asia

• Gene Location: Start: 70; End: 1752 Strand: +

Full Sequence      

MQRKLVEIFRALDLEKEYTKEEIMEWYLNVVYFGEGASGVQMAANTYFGKDVRDLTLAQC
ASIIGITNNPSRYSPFASVENNKKRTKTILYEMYDQDYISHEEYTAAVAEVDNDELHFIR
GENEVYQQEIYSYYVEMVIKDVLSDLQSRLGLSEAAARQLLYHGGYQIYACVDPFVQQVV
DDIYIDPDSMPQPYYYSSQPLQSACMVIDPFTGEIKAIGGAIGEKDRNFGFNYAIDAHRP
SGSSAKPLASYGPAMDLGLITESTLVDDSPDIQLRGTYWYPRNSGSSYDGIITIWDALVR
SKNTVAAQIIDKLTPSVSFEYLTERMGFQLVEADRDYAPLALGQFTYGVTVREMAQAYTT
FPNDGEMTYARSYSLITDSNGNTVLENGMRTSHVFSVDTARCMTEMLEAAAWYGTGYEVY
LGYGKMPVAGKTGTTSDDYDRWFCGFSPYYVTAVWTGYDNPAPMYYSGNPAAQIWRRIMS
QINEPLPVIQFNDPTNRGYDTGIFGDLETTPEPTPVPTATPAPTEAPTPEPTAEPTPEPT
PELQQIPVMPQETAEAAPEG

Enzyme Prediction     

No EC number prediction in MGYG000000709_00958.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	1	94	5.5e-34	0.5254237288135594

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	1.77e-134	3	484	75	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	7.45e-119	3	547	150	661	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	4.82e-88	3	496	140	732	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG4953	PbpC	4.37e-48	4	482	131	561	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
PRK14850	PRK14850	1.99e-47	3	489	230	698	penicillin-binding protein 1b; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAK99350.1	3.00e-120	1	496	172	686
QNL43378.1	1.86e-118	1	496	168	662
QBB65500.1	6.39e-115	1	494	143	646
ALP94484.1	1.30e-114	1	494	175	678
BCK85139.1	2.01e-113	1	496	169	694

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZG8_B	1.80e-58	13	466	2	440	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3ZG7_B	8.79e-53	13	466	2	440	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
4OON_A	1.13e-45	3	494	112	734	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
7U4H_A	5.58e-43	1	495	109	773	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3UDF_A	5.55e-36	1	487	110	720	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q97GR5	9.32e-81	2	494	174	678	Penicillin-binding protein 1A OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=pbpA PE=3 SV=1
A7GHV1	3.45e-78	1	496	151	657	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A5I6G4	1.88e-77	1	496	151	657	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7FY32	1.88e-77	1	496	151	657	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
Q8XJ01	5.35e-69	2	486	167	669	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000030	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000709_00958.