CAZyme Information: MGYG000000712_00096

Basic Information

• Species: Fusobacterium_B sp900541465
• Lineage: Bacteria; Fusobacteriota; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Fusobacterium_B; Fusobacterium_B sp900541465
• CAZyme ID: MGYG000000712_00096
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
752		87473.52	9.6148

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000712	2138816	MAG	Kazakhstan	Asia

• Gene Location: Start: 26481; End: 28739 Strand: +

Full Sequence      

MKIIFKIILFFTIIVFIITCGIYVRFNAENTEKIFEDRYSVSVLDNNGNIIGVYLNKDEQ
WHLKSTDEIPEKLKRAVMVFEDKNFYSHKGVDFSAVIRALKDNIFEKRRTGASTITMQAV
KMAQPKERSYFNKYLEIVHAFKLEKYYKKDEILKMYLNNAPYGGNTVGYKTASYMYFRKS
PKELTWGEAALLAVLPNSPGLMNVEKNREKLIEKRNFLLKKLYEKNFIDERQYRISLKEP
VPDKRYSFPVLAPHLARRLVNENKDKKIIKSTIDSEIQKKTEKAVKDYSEFLKLSGINNA
AVLIINNKNYEVTAYAGSQDFYDFENNGQVDGITAKRSPGSLLKPFLYAKVIDEGIAAPQ
SKIPDVPLYFSNFSPQNANKKYYGMVEMRDALIKSLNIPFVELLKDYGEDKFFYFLKDIL
NFEDKNPERYGLSLILGTKEFTMEEIGILYSGLANYGNFKNLKYTEESTEEGRQLLTKGA
SYLTLITMRELERPGMEKFYREKNPVSWKTGTSHGRRDGWAAGVTPEWTVVVWTGNFTGE
GNPNLTGVYTGGNLLFNILKFLPKTEKEFVMPNDLEKIKVDRETGYRLKYDIPYKEIFYP
KDAKPLKVSPYYKKIFVNKNGEETDSRDESFNEREEKYILLYPLEVVNYFVRENFDVSNI
VSEKTAEKTIKFIYPKNNLKIIVPKDFDGEKSLIVKIANIKNRELYWYVNKEYAGKSREK
ERSFNFKKGNYEITVVSEKGETAKINFQVAEK

Enzyme Prediction     

No EC number prediction in MGYG000000712_00096.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	59	222	2.6e-52	0.9322033898305084

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4953	PbpC	0.0	7	749	3	731	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
TIGR02073	PBP_1c	0.0	35	749	6	726	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	1.23e-104	2	552	13	583	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
PRK11240	PRK11240	9.22e-98	70	535	72	535	penicillin-binding protein 1C; Provisional
TIGR02074	PBP_1a_fam	2.87e-83	67	536	8	501	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SQJ06920.1	0.0	17	749	17	750
AVQ26788.1	0.0	17	749	17	750
AVQ31509.1	0.0	19	749	19	750
VEH39720.1	0.0	19	749	19	750
BBA50599.1	0.0	19	749	19	750

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FGZ_A	1.79e-34	70	586	165	717	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	1.94e-34	70	586	186	738	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3UDF_A	2.92e-33	68	457	46	539	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3DWK_A	2.17e-32	36	535	3	556	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
2OLU_A	1.59e-31	31	535	7	565	StructuralInsight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Apoenzyme [Staphylococcus aureus],2OLV_A Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus],2OLV_B Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P76577	1.02e-73	43	535	45	537	Penicillin-binding protein 1C OS=Escherichia coli (strain K12) OX=83333 GN=pbpC PE=1 SV=1
P40750	2.83e-44	45	538	60	599	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2
P38050	3.31e-43	40	624	48	664	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
A7GHV1	7.59e-36	52	618	64	691	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
P39793	6.81e-35	32	535	68	613	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.540551	0.117260	0.334634	0.000834	0.000507	0.006221

TMHMM  Annotations     

start	end
7	26