CAZyme Information: MGYG000000724_00055

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger
• CAZyme ID: MGYG000000724_00055
• CAZy Family: GT4
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
408	MGYG000000724_3|CGC1	44874.66	7.8546

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000724	1919940	MAG	Kazakhstan	Asia

• Gene Location: Start: 15631; End: 16857 Strand: +

Full Sequence      

MPKRILVVTQHFWPENFRINDIVEGFVQDGLSVDVLCGLPNYPKGEWFDGYSADGPFEEV
YKGARVFRAREIPRKGNTGKTIFLNYVSWPLYAAGALKRLPGGYDAVFCFNTSPVLMCWP
AIRYAKKHHVPFTNYVLDLWPENLYSVLPVKNGFMRWVAQTVSDTLYKRCDRLIAMSEPL
QKRLCARTGKSAAEIAVIPQYCEDFYAVPQHDAALEAQFAGRFNLVFTGTFTPAQSLDMV
MNAVLDARAAGAEKLHLLLVGDGMSREALQQQAEALHAGDAVTFYGSVPATDVPKFTALA
DALLISLSDSPDLGLTVPGKLASYMAAAKPVVASMNGAGYAAVKESDGGLVCPACDRKAL
ADNLLALYRMSENERAALGAKAKAYYAAHYRRAELLKRLEEFTLTGKT

Enzyme Prediction     

No EC number prediction in MGYG000000724_00055.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT4	221	375	6.8e-17	0.925

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03794	GT4_WbuB-like	5.41e-79	4	400	1	391	Escherichia coli WbuB and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. WbuB in E. coli is involved in the biosynthesis of the O26 O-antigen. It has been proposed to function as an N-acetyl-L-fucosamine (L-FucNAc) transferase.
cd03801	GT4_PimA-like	2.34e-26	4	402	1	364	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
COG0438	RfaB	4.73e-26	56	402	42	373	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
cd03798	GT4_WlbH-like	9.24e-20	30	369	31	342	Bordetella parapertussis WlbH and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. Staphylococcus aureus CapJ may be involved in capsule polysaccharide biosynthesis. WlbH in Bordetella parapertussis has been shown to be required for the biosynthesis of a trisaccharide that, when attached to the B. pertussis lipopolysaccharide (LPS) core (band B), generates band A LPS.
cd03817	GT4_UGDG-like	1.67e-17	121	401	100	370	UDP-Glc:1,2-diacylglycerol 3-a-glucosyltransferase and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. UDP-glucose-diacylglycerol glucosyltransferase (EC 2.4.1.337, UGDG; also known as 1,2-diacylglycerol 3-glucosyltransferase) catalyzes the transfer of glucose from UDP-glucose to 1,2-diacylglycerol forming 3-D-glucosyl-1,2-diacylglycerol.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWC11812.1	6.34e-114	3	404	4	407
AOT05155.1	2.19e-109	4	406	6	410
ABY23076.1	2.74e-107	4	404	6	408
ADX73677.1	1.41e-104	4	404	6	408
ASN82979.1	6.06e-82	5	400	3	396

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000046	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000724_00055.