CAZyme Information: MGYG000000733_01413

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; Borkfalkiaceae; Borkfalkia
• CAZyme ID: MGYG000000733_01413
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1481		165706.84	4.1506

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000733	1893651	MAG	Kazakhstan	Asia

• Gene Location: Start: 1115; End: 5560 Strand: -

Full Sequence      

MQLKKHFQITAIIVGILLALSLLIVACAKTDDGGDAGSEAGEFQLIDNSRAALIVVEGTN
APGATDGDYPGVVRAAYDLQEDIETVTDVKPVIAQSVTGYTDYAIIIGTIGKSEAIDKMI
ESGKIDVSQVEGKWETYLLETVDDPFGDGKVGTALVIAGSDKRGSIYGIYNISEQLGVSP
WTFFADSVPAHRDSFTLAADYRYVSEEPDVQYRGIFINDEEDLELWGRVMDDGKHMGPKL
YEEIFEMLLRCKANYLWPAMHACSDAFVDYVENPINADYYGIVIGTSHCDMLLRNNLNEW
DNFVSKYKSEHPEYANVNVLYDYTDKASQPVIEEYWRESVRANKDYEVSWTLGMRGAHDE
GFNTAYINQAPWYGDKNMLLEQIIDLQRTILMEELGVDTLEDVFMVFQPYKEVQELYNNG
LEVPEDITIIWADDNHGFVRNVPNAEERERSGGNGVYYHASYWGPDNESYMWINSMPQTM
VYEELSKAYKSGIQKAWVLNAGDVQMNMMEIGFVMDLGYDIDQYNNENVFEQYYGKVAAR
EYGEEFADEVVAIMKEYTQMTNVRKLEHMSVDLFSAAYGDEMERRASQYKDLWERAEALY
EAIPERQQNVFYETILFEVRCAYFINAEFYYAHKGNVAYEQGRNATAWNCYNYSLEFNRM
RKDEISYYNTLLNGGKWENMMDPENYAPPVMSGFANGGPTFALGATEPGVIAEGESLEQQ
NSSLSFYNYAQGRKYIDIFNKGSGNFNYTVSADKDFVKLSSTQGVVSDEVRVWVEIDWSK
ISKNETATVTITSGNYTKTIAINVVFGDYDLGEGTYVEQECYVSIEAEHYTREKETDAAS
WEVIKDLGRVSGDMVRAESNTLAGYNLDTFASSAPYLEYDVYFTSTGTFEMEVYRLPTLH
SQGHVRLAVGVGNAAPVVIEGERDYGTNNPAWEEGVFTQIIKHTVSITIENAGINTIRLY
MIDPWIVVDKLVIYTSEKQDSYFGPLESYNTTYNTDPAANAYYDAFYGTSGETYTLPVGY
DLAETYGTGYFVEQGGKLTIEPELAAEESEYAYTDGRWFPAVTDAGISMRTADLREDYNG
NTGAAPTMNFEIIITTPGTYNFWANVQSPAPRSSSYGYSINNGVVYTQGEYAYDREEVFH
WRKAGTALSFSTAGKYTLTIYCIQDGVSIEKIYMTRTGETPTSDSVTASARTTIWNASDP
AATAADSALRSELMAAASALGNSYAIPAGDRLGEYKQADVDALREAIDAVYSLVNKTSAL
TEGETNAAIEALNAAYAAMNAGRNMSADGKNYLVYEDYEKSVAGMQAYGVEINALSLTPD
MIVRRAADGTQYLNVRAFQAQRNMQKAFLQYSFAPQSGELTVEARMSFNEADWGNAIYLL
NEDGEYSVSIAFEYAYGKYNLVAYNAGVKTVLAEYTRNEMVDIKVVTDVESGRFDVYVNG
ELKADGFRYRNSADTLNAVMFGSSAKNSDLRVDSIAVYTAE

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	55	687	2.3e-187	0.8192252510760402

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	1.84e-123	207	568	2	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	1.67e-49	815	987	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
cd14948	BACON	6.01e-05	722	803	7	81	Bacteroidetes-Associated Carbohydrate-binding (putative) Often N-terminal (BACON) domain. The BACON domain is found in diverse domain architectures and accociated with a wide variety of domains, including carbohydrate-active enzymes and proteases. It was named for its suggested function of carbohydrate binding; the latter was inferred from domain architectures, sequence conservation, and phyletic distribution. However, recent experimental data suggest that its primary function in Bacteroides ovatus endo-xyloglucanase BoGH5A is to distance the catalytic module from the cell surface and confer additional mobility to the catalytic domain for attack of the polysaccharide. No evidence for a direct role in carbohydrate binding could be found in that case. The large majority of BACON domains are found in Bacteroidetes.
pfam03648	Glyco_hydro_67N	3.71e-04	42	170	4	118	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASA21655.1	1.93e-276	8	1462	7	1481
QUC63565.1	1.67e-205	68	997	45	956
ANS63447.1	1.82e-201	4	998	15	985
AXG52369.1	1.82e-201	4	998	15	985
QMV12190.1	1.92e-201	21	998	12	964

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	4.38e-131	34	988	1	933	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
6NPS_A	1.12e-98	71	989	31	964	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
5BY3_A	1.65e-90	45	777	21	714	Anovel family GH115 4-O-Methyl-alpha-glucuronidase, BtGH115A, with specificity for decorated arabinogalactans [Bacteroides thetaiotaomicron VPI-5482]
4C90_A	4.57e-82	54	681	46	640	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	4.80e-82	43	681	17	625	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000003	1.000039	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
7	26