CAZyme Information: MGYG000000745_01402

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74
• CAZyme ID: MGYG000000745_01402
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2437	MGYG000000745_27|CGC1	268624.97	8.5157

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000745	2615728	MAG	Kazakhstan	Asia

• Gene Location: Start: 11472; End: 18785 Strand: +

Full Sequence      

MRLFSVADVRPLGYARLAPPRGLPNALRRTLRALRAANRDALSPAACCLEDNAPFLLDEW
DSLRRTLRAAPALPAVNGRPRILLVAEKIRDEAQDDLSMPAIVRIARAAQGDFPFTQREL
SLLRPALQRALMDNVFSLAAQCRAEARRLRDAESWLRRAARGETPAPPRSAAVAEALAER
LAADGGAALDIPPENGIPAEALLRDAGEKKAANGMMAGQLISSLRRLNQMSFERILRRLD
PVEAILRADATFRRMDAAGRERYRRAAARIAHRLRTDECAVARAACALADGKSGLEREAG
YYLLEKSDEIAKALFRRGGGFLARHQTGAFLACLYGGTALLAAAAMLVAPWPLLLPVALC
ASELVRLLVYRAARRLFPARALPRIQIRRLSRERRTLVVVPTLLTSPKQALRMTRQLALL
HAAQPDPYLDFMLLGDFADSPTQSLPADEEILDAARAGVAALNRAHDGGFYYLQRARTWD
AGQNAFTGKERKRGALEALNRLLCGRETPAFLYASCDVNTLRRRYAYVITLDADTFMPPG
AAYKLVGAMEHPLQKGRVAVIQPRMAVAGDRVKTNVQRLLGGRGGTDPYGMAVQDVYQDV
FGRGSFVGKGIYAPEAWLSALENRLPAGRLLSHDLIEGECAGSMLAEDIVLYDGHPMRLS
GWQKRLHRWTRGDWQLLPFLFDRRLSLLSRHKIWDNLRRSLVPAAQVALLLAGAFGHPAL
LLLALPWPLRGLGVRLTLLPGKALTQLDAALRALYRQFVSKKKLLSWVTAAQAENGDAPA
LSCVLVQLAAGAGMLVCSLLPQGFEPAVFIGMLWTASPLLLRYADAPTQREPGMTDAQKT
AVRALARDTWRFFEDCVTEKTRFLPPDNVQLEPDKGAAMRTSPTNIGLYLLSCCAARELG
LITTRDMAVRLSRTLGALSSLRRWRGHCYNWYDLETLDPLPPRFVSTVDSGNLRACLIAC
AQLCRQRLAEMPQEYLPLPAQMDALAAQMDFSPLYDRGAGLFYVGLGAENEKPTGAHYDL
MASEARMASFLAVASGQAPRRHWARLGRRTVRAGGGPSLLSWGGTLFEYLMPALLMPLSP
GTLLRESCLNACRAQMLAAGGRPFGVSESGYYAFDPELNYRYRAFGLPLLALSAETGGDV
VAPYASMLALPFYPRACAENYLRMRRLGWTDAHGLYEAADYGAGERNRAPRLVKSHMAHH
QGMILCAVCNALTDFSLVRAFMAPPWIQAYAYLLCESAPTRAGRRRTLPPPRRETQESGR
QWKIAAQDMPPDTQLLFGNGTTWALTAHGMGYLKHSGMMITRFWETLQIPAGPQFFIRRE
DTGDVWRMGERDKAIMTEGGMRFERENDDCALQWRCAVDPLSGWAVAALRVENKRPDTLT
LSAISYLEIAQGALSDDLSHPNFRDLSVRVEEWDGAQGLLSRRLPRSARDETPLILHAVI
GDVAALRRQGDRRLFLGDGDAAHPAQAALPADACAYRIGDVIAPCLSLRAEMRLPAKGRA
QLYFITAVRDRPPEPPLLSQAKSALALAATQAQMTLRFLGMDESTLSLCQQLAGALLYHH
QAHQLCLPSAPPDALWRLGVSGELPVLLLLMTNDCDKASLRLLLRCHAWMRLQGVWVDFV
ILCPGETGYFNAARDMAAAMIAASPARDWQGIPGGVHLSVASPEEAEGARLLARVTLRGG
APIKGQLAALRRIALPLQTDNTLPLPITPPALSIENGYGGFTDEGDYIITRRPPAPWHLL
LCNELFGTLLSDRGVLQSYYVNSRLGRLTRPAPDVHAAFRSEEMLLRTESGACASTMGGT
IRFSPGEAACDGYLDGVYFTLSVFTHPVLPLGVRLLTLRTEGQKRLRLLWRVRFDMGERP
ESTRYETDGAMGFARCGDMPGVAFGCLHGASSATDFDHALVSWQMDEPLAPHAAKTLACL
IGFAPDKASAREMARRAEEEGSAALRETRRQWAARLDGLRLFTADQSWQWMLRWLPYQAW
TARLLARMGPYQAGGAYGFRDQLQDVLCLMHTAPDVARAHILLCAAHQYEEGDVQHWWHA
PRRGVRTRISDDKLFLPFLTAVYVQITGDESILQARAPYLSSPPLAPDEGDRYEEPDETD
RAEPLLCHCLRAIDSVALGRHGLPLMGGGDWNDGMNRLGGKNGESVWLGFFLCWTLRLFA
PLCAAPEKERLDALRRALLDAAEAAWADGWYLRAWADDGRPVGGPATDPPRIDLISQCFS
VFAGAPRQRAREAVLAAYSRLYDREAGVVKLLDPPFTPEEGAGYIGAYLPGIRENGGQYT
HAVPWLVLALCDLGENARAWEIAEAILPPRHSDVKARADVYQLEPYVLCGDVSAGQNMGR
GGWSWYTGSAAWLYFVTVTRLLGFEKRGDRARLAPCPHPNGEEYTLVYRFGGASYHFTAA
ADALFPTLDGEKLPQDSFVSLIDDGRTHEARYPLRAN

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1522	2430	2.8e-190	0.917953667953668
GT84	1017	1225	1.8e-66	0.9906976744186047

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	2.80e-153	1451	2414	5	1034	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	4.62e-89	1952	2363	2	423	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
cd11753	GH94N_ChvB_NdvB_2_like	2.23e-30	1217	1508	1	310	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	1.44e-23	1285	1506	17	246	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.
cd11756	GH94N_ChvB_NdvB_1_like	1.09e-13	1711	1937	1	283	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACG74515.1	0.0	15	2436	57	2737
ABC82952.1	0.0	15	2429	57	2735
ACL66712.1	0.0	15	2429	57	2735
AHG93687.1	0.0	36	2431	108	2810
AVX19642.1	0.0	200	2428	244	2760

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3QDE_A	5.91e-46	1939	2429	295	805	ChainA, Cellobiose phosphorylase [Acetivibrio thermocellus],3QDE_B Chain B, Cellobiose phosphorylase [Acetivibrio thermocellus]
3S4C_A	1.68e-40	1939	2398	302	782	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]
3RRS_A	9.08e-40	1939	2398	302	782	Crystalstructure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RRS_B Crystal structure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RSY_A Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3RSY_B Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3S4A_A Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4A_B Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4B_A Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda],3S4B_B Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda]
2CQS_A	5.12e-38	1939	2398	322	802	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]
3AFJ_A	6.77e-38	1939	2398	322	802	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127],3AFJ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	2.19e-272	395	2403	488	2809	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
B9K7M6	1.40e-45	1718	2428	3	806	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1
Q9F8X1	3.25e-34	1943	2398	288	760	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1
Q76IQ9	6.49e-32	1952	2396	297	758	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1
Q8P3J4	1.80e-27	1896	2434	260	796	Cellobionic acid phosphorylase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=XCC4077 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000057	0.000002	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
2342	2364