CAZyme Information: MGYG000000747_00299

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74
• CAZyme ID: MGYG000000747_00299
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2466		268920.41	8.0536

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000747	2418810	MAG	Kazakhstan	Asia

• Gene Location: Start: 18618; End: 26018 Strand: -

Full Sequence      

MDHASSPLSLIALLPPARISVRIRRAHAACRRVPREELSPAGMWIEDHARFLLQEALRLR
EALKNAPCLPGSGGQPRVARLAQEICREGSDQVSAAAILRISRAFFADGEPAQQELCMLR
DALACALLDRLGPVLHACMEEAERRRQAERFTARLGRGKAPGLPENPALQAQIIVRLSAA
ENAEAMQQLDGLLQKGGVAAREAMARHQAEQTRLGQEAGSVIAALHALERLPFDRIIERL
SPISAVLRQEETYRRMDAESRNFYIARVCLLARRLHVGESAVARAAMALREGNEGPRAQC
GFYLIEQPEAIGAYLLSRKPGFFARHREGAFSLALGLGACVFLALGMLFGAPWYTFPGIL
LCGSELTRRCLHALLRRRFPARLIPRLRIRALSPAQRTLIVVPTLLVSRKQALHMARQLS
VLRCASPEKNLEFMLLGDFADSAAEAQPQDQELLATASAAVDALNAQYGGGFFYLHRARR
WDGGQRRFTGRERKRGALEALNLLLTGRECPDAFDYASCDLKFLRERYAYVITLDADTFL
PAGAALALIGAMEHPLQKGRIGVIQPRMAVAPDTVRTRAQRLLGGRGGADPYHLSAQDLY
QDVFGKGSFVGKGIYQPAYFLDKMAGRVPAGRLLSHDLIEGEIAGSALAEDIVFYDGHPA
RLSGWQKRLHRWTRGDWQLLPFLGDRRLSLLSRLKIHDNLRRSLLPAAQAGLLLLGAGLD
LPLLCLLGLPWPIRGMGLRLLLLPAKAYTLLDAAIRALYRQLVSHQGLLSWVTAAQAEST
GGYPLPCVLAQIGAGTGMIILSLLPGGFLPAVFVGLWWVLGPLLGRALDAPEKTEPALTP
VQREDARALARNTWQFFERHVTKENHFLPPDNVQLDPDKGEAHRTSPTNIGLYLLSCVAA
RELGLITTAQMASRMDETLATLESLETWHGHLCNWYDTQTLSPLPPAFVSTVDSGNLAGC
LLCCAQACRERLGEMAEGYRALPARLDALATAMDFSTLYDKEAGLFAIGYDLSAQRLAPA
HYDLLASEARLASFVAVMEGQVPRRHWQRLGRSCVRAGGGAALLSWGGTMFEYLMPGLLL
PLIPGTLLGEGCMYAIKAQISAAGQRPFGVSESGYYAFDPDLNYQYKAFGLPALALSGET
AGSVVAPYASMLAMPYTPRAAGANLAHMRRLKWLDDQGLFEAADFSPQRVGKGPGLVMSH
MAHHQGMILCALCNALTDFSLVRHFMSPPRAQASVDLLWERPSPLPRRQIFLPPPREESY
AGGAFPRAAREGLPVDAHALFGGGTTWLLSGNGQGYLSHQGMLITRFFPEADQPSGPQFY
LRDEKSGGCIRIPVRGKAVFSPGKVDFQAQAFGLNAALQCCVEPLTGMAVAALTLENPGK
EAREASVCSFLEIAQGDRAADEAHPNFRDLSVQIAPFGSSGLLSRRLPREEKDEMPLICH
TAAGDIAALRRQGDRLLFLGRDGSYDQPAQLQPGGDETACRLGDVIAPCLSLRALVRLPA
GGTAHLYFFIAVGKTEEALAALSFTPAFARSAFSLSATQARMALRFLHMDGRMLALYQQL
LGAVSFSGQPHQCALPSAPPAAFWRFGIAGMLPVLLVRLHAADKPLIRHVLRAHSWMRAQ
GVWIDLVFACQEEGGYARPVQDTVNALLSASPDRELRGASGGVHVVACGEEESAAMESRA
ALTLRSGMALPAQLSALRLSPPAAGAALTLPIPVAPPPLWLDNGFGGFTPDRDYAVYALP
PAPWHNILANGQFGTVACESGVLQSFGENSRLSRVTRPAPDVHRPRPAEEIYLRCPDGRL
YPLCRPTALHAPGVTTYQTKAGDVYAEVSLCCPEGMACGARIVTLRSEKPQKLRLHYLVR
FALGEQPAFTRCRVEGGFAFARSGDSPIGWAALDGGAARTLCPALCFGLQGQEAPCLSPS
GPDQGSMALCQRDLTLKPHEPLQLLLAVGIADSPDAAQAQFAALLRQGGGDLLRAVRGQW
DRRLSALCLYAADAPLEAMLNRWLPYQALAARLLARTGPYQTGGAFGFRDQLQDLLAVLH
TDPAFARAHILRCAAHQFPEGDVQHWWHAPQKGVRTRISDDKLFLPYLTALYVRITGDAA
ILDADAPYLLSAPLQPQEDDRYEEPAQSAWQEPLLRHCLRAIDSVALGMHGLPLMGSGDW
NDGMNRIGGRQGESVWLGFFLALVLRHFAPLCPEEERARLLRLRHQLLENAESAWAGPWY
LRAWNDAGQPIGGPDTQPPRIDLISQAFAMLAGAPRDHARSAVLAAARLLYDRAHGIVKL
LSPPFALPENAGYIGAYIPGVRENGGQYTHAVPWLIAALCQAGESALAWEIARALLPLSH
SDTPENARVYRLEPYVLCGDVYAGQNPGRGGWSWYTGSAAWLYYVWFTVLLGFEKQGNQA
RLVPQPAPNQEEFTIVYRFGTAEYHFTAARDVVFPTLDGEKLDGGWATLQDDGRTHECRF
PMKRPQ

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1484	2458	2.2e-195	0.9652509652509652
GT84	1021	1229	5e-70	0.9906976744186047

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	1.02e-161	1454	2456	5	1047	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	4.25e-89	1980	2392	2	423	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
cd11753	GH94N_ChvB_NdvB_2_like	6.62e-39	1221	1535	1	336	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	4.01e-26	1288	1510	16	246	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.
cd11756	GH94N_ChvB_NdvB_1_like	1.30e-21	1718	1955	1	274	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVX30049.1	0.0	31	2456	56	2760
AVX19642.1	0.0	31	2456	56	2760
ABC82952.1	0.0	36	2465	86	2743
ACL66712.1	0.0	36	2462	86	2740
ACG74515.1	0.0	36	2462	86	2735

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3QDE_A	4.60e-47	1976	2462	304	810	ChainA, Cellobiose phosphorylase [Acetivibrio thermocellus],3QDE_B Chain B, Cellobiose phosphorylase [Acetivibrio thermocellus]
3S4C_A	1.19e-46	1725	2455	3	815	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]
3RRS_A	6.61e-46	1725	2455	3	815	Crystalstructure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RRS_B Crystal structure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RSY_A Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3RSY_B Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3S4A_A Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4A_B Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4B_A Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda],3S4B_B Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda]
2CQS_A	5.66e-45	1934	2455	273	835	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]
3AFJ_A	7.51e-45	1934	2455	273	835	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127],3AFJ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	2.71e-285	21	2431	70	2808	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
B9K7M6	1.48e-50	1731	2462	10	812	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1
Q76IQ9	8.20e-35	1725	2444	3	783	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1
Q9F8X1	1.08e-34	1725	2443	3	782	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
329	351
707	729
799	821
2371	2393