CAZyme Information: MGYG000000810_00101

Basic Information

• Species: Sutterella sp900543805
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Sutterella; Sutterella sp900543805
• CAZyme ID: MGYG000000810_00101
• CAZy Family: GT9
• CAZyme Description: Lipopolysaccharide heptosyltransferase 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
325		36021.2	7.0295

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000810	2278510	MAG	China	Asia

• Gene Location: Start: 108022; End: 108999 Strand: +

Full Sequence      

MKILVVKTSSMGDIIHALPVTVDIRENLPDAEVHWLSEESFREIPALSPVVSKVHVCAFR
RWRKNVFSAQTLAEVRALRAALAAERYDVVIDEQGLLRSAWPASWTRAPVHGFSRDTVRE
PAAAFFYRHPHRIPEEVGAVKRYRLLAAETLGYEVPAHAPAFGLRPRAQALEVPEGPWVA
LAVNASRDEKLWPEDHWVDLGRSLLEEGRRSVFFWGNDKERERVERLARFIPDSLVAPRA
RLDRVAATLVKAEALVGVDTGLSHLAAALGLPTVGLYVSTPTEILHLVGDGPVRSLGNVG
EIPAWADAKTALDAVRQEQKENACS

Enzyme Prediction     

No EC number prediction in MGYG000000810_00101.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT9	76	282	6.5e-36	0.8266666666666667

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02193	heptsyl_trn_I	1.03e-113	2	315	1	318	lipopolysaccharide heptosyltransferase I. This family consists of examples of ADP-heptose:LPS heptosyltransferase I, an enzyme of LPS inner core region biosynthesis. LPS, composed of lipid A, a core region, and O antigen, is found in the outer membrane of Gram-negative bacteria. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]
PRK10964	PRK10964	2.82e-79	1	280	1	282	lipopolysaccharide heptosyltransferase RfaC.
COG0859	RfaF	1.35e-62	1	304	2	304	ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01075	Glyco_transf_9	1.90e-46	85	321	11	236	Glycosyltransferase family 9 (heptosyltransferase). Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.
cd03789	GT9_LPS_heptosyltransferase	6.68e-40	2	293	1	238	lipopolysaccharide heptosyltransferase and similar proteins. Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF22155.1	1.78e-118	1	315	1	315
QQS89702.1	1.13e-117	2	315	3	316
QDA54731.1	7.73e-116	2	319	6	323
QRM18003.1	1.03e-86	1	314	1	314
QDC48452.1	3.88e-82	2	315	12	324

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2GT1_A	2.41e-59	1	323	1	320	E.coli heptosyltransferase WaaC. [Escherichia coli UTI89],2GT1_B E. coli heptosyltransferase WaaC. [Escherichia coli UTI89]
6DFE_A	2.41e-59	1	323	1	320	Thestructure of a ternary complex of E. coli WaaC [Escherichia coli],6DFE_B The structure of a ternary complex of E. coli WaaC [Escherichia coli]
2H1F_A	3.00e-59	1	323	1	320	E.coli heptosyltransferase WaaC with ADP [Escherichia coli O6],2H1F_B E. coli heptosyltransferase WaaC with ADP [Escherichia coli O6],2H1H_A E. coli heptosyltransferase WaaC with ADP-2-deoxy-2-fluoro heptose [Escherichia coli RS218],2H1H_B E. coli heptosyltransferase WaaC with ADP-2-deoxy-2-fluoro heptose [Escherichia coli RS218]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P24173	3.04e-58	1	285	1	287	Lipopolysaccharide heptosyltransferase 1 OS=Escherichia coli (strain K12) OX=83333 GN=rfaC PE=1 SV=1
P26469	7.73e-55	1	299	1	307	Lipopolysaccharide heptosyltransferase 1 OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=rfaC PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000810_00101.