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CAZyme Information: MGYG000000851_00406

You are here: Home > Sequence: MGYG000000851_00406

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA7173 sp900552325
Lineage Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; UBA7173; UBA7173 sp900552325
CAZyme ID MGYG000000851_00406
CAZy Family GH32
CAZyme Description Levanase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
341 MGYG000000851_24|CGC1 37044.59 4.5973
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000851 2227060 MAG China Asia
Gene Location Start: 65;  End: 1090  Strand: +

Full Sequence      Download help

MFWNKETGEW  NLLLAHALEH  EMLVFTSPDL  REWTLQSAFG  KGLGCQDGVW  ECPDLFRLPV60
DGTDTQKWVL  LCNINPGGPF  GGSATQYFIG  DFDGKTFKPD  TLADGSVPTK  WLDYGKDHYA120
TVTWSDAPDG  RRTAIGWMSN  WQYAAEVPTM  QFRSANTLPR  ELGIFTGDDG  DLYVSSAPSP180
EVDALRGKPT  VSTSFTVAGA  GKRFTLPAAN  DGICEIALDL  EPLSASAITL  TLSNSGGEKA240
VVTYNPSEQT  LSMDRTQSGI  TTFSQDFPAV  TVAPVRNGGE  TGLRLFVDRS  SIEVFEKRGR300
AVMTNLVFPE  SPYTTLTVSS  TGGKAKVKNL  RIYPLTPAAN  D341

Enzyme Prediction      help

EC 3.2.1.26 3.2.1.64 3.2.1.80 3.2.1.65 3.2.1.153

CAZyme Signature Domains help

Created with Snap17345168851021191361531701872042212382552722893063233167GH32
Family Start End Evalue family coverage
GH32 3 167 7.4e-40 0.5119453924914675

CDD Domains      download full data without filtering help

Created with Snap17345168851021191361531701872042212382552722893063232163GH32_Inu-like2337SacC2295Glyco_322173Glyco_hydro_32N181334Glyco_hydro_32C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
cd18622 GH32_Inu-like 1.18e-77 2 163 135 288
glycoside hydrolase family 32 protein such as Aspergillus ficuum endo-inulinase (Inu2). This subfamily of glycosyl hydrolase family GH32 includes endo-inulinase (inu2, EC 3.2.1.7), exo-inulinase (Inu1, EC 3.2.1.80), invertase (EC 3.2.1.26), and levan fructotransferase (LftA, EC 4.2.2.16), among others. These enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
COG1621 SacC 5.37e-75 2 337 168 486
Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism].
smart00640 Glyco_32 1.30e-66 2 295 140 433
Glycosyl hydrolases family 32.
pfam00251 Glyco_hydro_32N 7.26e-49 2 173 137 305
Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure.
pfam08244 Glyco_hydro_32C 4.33e-36 181 334 1 162
Glycosyl hydrolases family 32 C terminal. This domain corresponds to the C terminal domain of glycosyl hydrolase family 32. It forms a beta sandwich module.

CAZyme Hits      help

Created with Snap17345168851021191361531701872042212382552722893063231335QCD36721.1|GH321335QJR98329.1|GH321335ASB38314.1|GH321335ANU64796.2|GH321335QQR09059.1|GH32
Hit ID E-Value Query Start Query End Hit Start Hit End
QCD36721.1 1.32e-169 1 335 276 612
QJR98329.1 7.02e-163 1 335 268 604
ASB38314.1 5.33e-162 1 335 274 611
ANU64796.2 5.33e-162 1 335 274 611
QQR09059.1 5.33e-162 1 335 274 611

PDB Hits      download full data without filtering help

Created with Snap173451688510211913615317018720422123825527228930632313401Y4W_A13323RWK_X13083KF3_A13083KF5_A13083U14_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
1Y4W_A 2.48e-64 1 340 173 518
Crystalstructure of exo-inulinase from Aspergillus awamori in spacegroup P21 [Aspergillus awamori],1Y9G_A Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose [Aspergillus awamori],1Y9M_A Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P212121 [Aspergillus awamori]
3RWK_X 2.65e-44 1 332 179 510
Firstcrystal structure of an endo-inulinase, from Aspergillus ficuum: structural analysis and comparison with other GH32 enzymes. [Aspergillus ficuum],3SC7_X First crystal structure of an endo-inulinase, from Aspergillus ficuum: structural analysis and comparison with other GH32 enzymes. [Aspergillus ficuum]
3KF3_A 1.07e-36 1 308 156 476
ChainA, Invertase [Schwanniomyces occidentalis],3KF3_B Chain B, Invertase [Schwanniomyces occidentalis]
3KF5_A 1.11e-36 1 308 159 479
ChainA, Invertase [Schwanniomyces occidentalis],3KF5_B Chain B, Invertase [Schwanniomyces occidentalis]
3U14_A 1.44e-36 1 308 182 502
ChainA, Fructofuranosidase [Schwanniomyces occidentalis],3U14_B Chain B, Fructofuranosidase [Schwanniomyces occidentalis],6S1T_A Chain A, Fructofuranosidase [Schwanniomyces occidentalis],6S1T_B Chain B, Fructofuranosidase [Schwanniomyces occidentalis],6S2B_A Chain A, Fructofuranosidase [Schwanniomyces occidentalis],6S2B_B Chain B, Fructofuranosidase [Schwanniomyces occidentalis]

Swiss-Prot Hits      download full data without filtering help

Created with Snap17345168851021191361531701872042212382552722893063231335sp|P05656|SACC_BACSU1340sp|Q96TU3|INUE_ASPAW1340sp|Q76HP6|INUE_ASPNG1340sp|E1ABX2|INUE_ASPFI1340sp|A2R0E0|INUE_ASPNC
Hit ID E-Value Query Start Query End Hit Start Hit End Description
P05656 6.08e-68 1 335 175 509
Levanase OS=Bacillus subtilis (strain 168) OX=224308 GN=sacC PE=1 SV=1
Q96TU3 2.04e-63 1 340 192 537
Extracellular exo-inulinase inuE OS=Aspergillus awamori OX=105351 GN=inuE PE=1 SV=1
Q76HP6 3.04e-62 1 340 192 537
Extracellular exo-inulinase inuE OS=Aspergillus niger OX=5061 GN=inuE PE=1 SV=1
E1ABX2 3.04e-62 1 340 192 537
Extracellular exo-inulinase inuE OS=Aspergillus ficuum OX=5058 GN=exoI PE=1 SV=1
A2R0E0 1.64e-61 1 340 192 537
Extracellular exo-inulinase inuE OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=inuE PE=2 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000071 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000851_00406.