CAZyme Information: MGYG000000867_01270

Basic Information

• Species: UBA1409 sp002338885
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA1409; UBA1409 sp002338885
• CAZyme ID: MGYG000000867_01270
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1915		210224.18	3.9234

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000867	1874565	MAG	United States	North America

• Gene Location: Start: 22278; End: 28025 Strand: -

Full Sequence      

MKKRALLILLSLIMTLNLVAMTGCAGNKDEGDADNLGAENVDYGRIYDGTKTATVLMDKS
YSASENSDRTYSQVLRAANDLRQDIAMVTGAIDYKEIQQTFADDEEKKLERLENADQSKA
PQLKTGSANAEYAIIIGVIGESELIDEIIESGSFDEAKSIEGSWEAYAIKEIPNPASGID
AALVIAGSDARGAIYGIYTISEEIGVSPFYWYSDVPVDVKDEIDVNYSTALVEDGPEVKY
RGIFINDEEKSTEWAKLKYPTENGSPDVNYYRHVFELVLRMKANTLWPAMHGSSTAFNKA
VDEDGIPINAQEAAAYGVIMAASHCEILLRNNVGEWGDWFNEHKSEYPDIKYPDDSYKAY
DFTLNREMLLDYWRERLETNKDFESILTLGVRGPHDEAFNCEDLSAYEGATEEQKKVSFM
EDVIQSQRQLIAEIYGEDMVTQIPQVLIPYKEMNDVYNAGLNEFMLWDGSEDFNGDGEIN
YLDDNTDIMLMWAEDNENYLRQDLTEAEANRPGGAGVYYHISYWGPPSSYLWLNSASLFV
MTEQMSRAYNIGADDYWILNVGDIKPSEPSMEYFLKMAWDPASFDGDSVGEYLTAQAIRD
YNLSEEDAKAMADAICEYYVINGSIRKAEFYANGDYNIHPVAFSVIANGDEGLLWVERLT
SLVDTVDALYQKMSDECKLSFYEHFYYNILSCLDIAEQFVYYEKNQLAAEQGRLGSANVY
AELALEAVQRTKDRLSDFNSLNDNKWTSFMDYAHPGKSKTLVGDSGETMSYVTQSVDTVG
AYCENGLRVGAGTLRLNSLIPSDTRYFDVFSCASIEKAWVAETSDDWIVLSQTSGATRTE
QRVLVSADWDKVSGTAEGEILIYNADENGNKIGVAVATFDVIAVNSDVEYGDHTGYIEAN
GYVAIEAEHYSEAVEGDDGRNWQVFVSNGRHGDTVKAMPDTAVKTEDWDSTAKLVYSVYF
ENAGTYKLTLERMPVLNEGTDETGAKRTMNIAVGVSDDEPQVLVGECEATLWGLRQSVMD
MHQKLSCNITVEQGWNDIVVYMSDASFVWDRMVIETVSGALRSTVLGPAESPNNIAPIGE
AKVASLPEELASYSPENEIVLSGLGNIRMSSGTEKTYELDVYATNGSEVKISAASDNPDV
ADVSIADGGYTVTASENTGSAALTFTFSAEGCDDLTYEISVRVSDPNQGTAYFEEGGEVV
IDAANALDESQYSWTDTDGNYEWTLTSDRDGLSIQPDTGVKWENTSDNLSGAPSLNYMVE
INNSGTYYLFVNMSAPNADADSYHVVVDGKYAYTHNSGDMSGEKVWKSASVGTELSAGEH
TITIVPREDGFVINQIVLTANKNASFTDGVLEGAESTAPSQSSDGDDGSSDSESIVVDSG
SIVIDATSALDESDTAWTDTTGEDEWVLSSDGCGLSIQPDTGAKWENTSENLSGAPSVNY
KIDVNSAGTYYLFVNMSAPDMDSDSYHVMIDGEFRYTHAGDPMNGDKLWKSAGRGIDLSA
GEHTITIVPREDGFVINQIVLTTNKNANFSDGVLVNSDAQASQAQSLARSAVWSTAFSSA
DKSLGGSLVRQQMRLSSSGDSVSLVISNQYGKSDLVIEGMSIAKLNEWGNYEIDSDTAVT
VTYNGDERIVVPAGTKISTDAVDFEFEPLECVGVTMSLSEVPQTITYHESARCYTWFGDG
ENLTQNDFSNGNTSTSWYFVEEIDVISDDVDTIICLGDSITDGYGIDNNTLSRYSDYLSE
LLADDPSLENMAVAAKGIGGDWMGAIESRFSRDVLNVNGAKYCVILAGINTIGFMSNNDS
ASVINQYKTWVDRCHAIGMKVIGVTVLPFKNSQYDDTSSGGSNYENAREPARLEINDFIL
NSGYFDAAIDLASMVADEDDPEIMDSKYTNDYLHLNSTGYELMGNAVYDCIKELA

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	70	758	3.1e-161	0.8020086083213773

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	3.02e-120	236	630	3	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
cd01830	XynE_like	1.59e-43	1712	1907	1	200	SGNH_hydrolase subfamily, similar to the putative arylesterase/acylhydrolase from the rumen anaerobe Prevotella bryantii XynE. The P. bryantii XynE gene is located in a xylanase gene cluster. SGNH hydrolases are a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.
pfam17829	GH115_C	2.14e-26	895	1070	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
cd04501	SGNH_hydrolase_like_4	5.87e-19	1713	1907	3	179	Members of the SGNH-hydrolase superfamily, a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.
pfam13472	Lipase_GDSL_2	6.53e-15	1715	1902	1	176	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUC17495.1	4.45e-163	46	1112	27	1050
APZ46852.1	4.45e-163	46	1112	27	1050
AFO77172.1	1.36e-146	74	1071	71	973
ADJ45460.1	1.36e-146	74	1071	71	973
AGT84300.1	1.36e-146	74	1071	71	973

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	1.91e-111	86	1072	24	934	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
6NPS_A	1.55e-93	130	1071	61	963	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
7PUG_A	8.24e-76	131	750	66	625	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	2.11e-74	131	750	65	624	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
4C90_A	6.96e-73	74	756	65	646	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000053	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000867_01270.