CAZyme Information: MGYG000000870_02178

Basic Information

• Species: Akkermansia sp004167605
• Lineage: Bacteria; Verrucomicrobiota; Verrucomicrobiae; Verrucomicrobiales; Akkermansiaceae; Akkermansia; Akkermansia sp004167605
• CAZyme ID: MGYG000000870_02178
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1289		143148.51	9.2042

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000870	3134704	MAG	United States	North America

• Gene Location: Start: 16895; End: 20764 Strand: +

Full Sequence      

MKSARAFLFSMLYLGPALPLLGEAPDWENEEVTGINKEAPRAAVLPAADKTMSLNGDWKF
KFSMRPDERPADFYKPTYSVSSWDTIKVPSNWQLYGYGTAIYTNVPYPFKPNPPKVTGEP
PKNWPAYKERNSVGSYRRDFNLPPSWKGEQVMIRFDGVESAFYVWLNGRKVGYSQDSYTG
GEFDLTPYVKPGRNTIAVEVYRWSDGSYLEDQDFLRLSGIFRDVTLFSQPQAHVRDLFLK
AGLDRKNYTTGILDGTFTIRNSGKKDIPAGMKLEYSIDGVSTNLNWEGKSGSGSVKTDNR
GRLDSGTLTVPAIPAGGEVQVSLKKEYPGVKPWTAETPNLYKVAYSLNGKDSRSVNIGFR
TVEIADNGAVLVNGEAVKFKGVNRHESHPDYGRAIPREVMEKDVQIIKAHNINTVRCSHY
PNHPYFYDLCDRYGLYVMDEANCEAHGIRNSGMDISRKPSWKKAHVERNMSMVHRSKNHP
SIVFWSLGNESGNGPNFVAASEAIRACDNTRPLHYCEFPHGHKAVDMDSAMYPPVDRVEN
WGKEKTSRPFFVCEYAHSMGNALGNFKEYMEAFESSPRMVGGAIWDFVDQSLRANPVGNG
IYKPAPFKGVTQAYGGMFGDRPNQANFCDNGIILGNRNTTAKTKEVKKVYQYMAFERQDG
KLTVRNKYFHKPLKGYTLYLVSLTPGSGHAVERVPLPEVAPGKSVTVSLPSAAMKSGSLM
VLADARYKLPEDVKMLSASQLEHVVDECEAYEWFPAPADKVSAVKAPASLPAVSVRKGNP
LVVQGKGFTATFKDGMLSALRYGGQDLILPDYPVTLQAYRSPVDNDSWIRSKVEGKMKMQ
NMKMECSDVQASVISPGVARVTAEFKTKGSELSLSGRITWTVFGNGIINASARIYPSAKG
EELLRLGFTFGMPAAYDQVEYLGLGPWDNYRDRRTSCWKDLFRTSVDDMFFAYSRPQDMG
NRMETDWVALSKDKSAPALWVGAASPGAPLEVSILRYTPRELNNAKSLDKLPEKNKVIVN
LDAFQMGLGGSSCGPRPLVKYQTLSEATPLGFVLAPASGLLNLARSGLLVPHSPVIERDE
DGMVSLSSSTPGVEMKYSVNKGPEKVYKKPFKLPEGNVRAWAAAGKPGSAAPTSPDERQF
PLIKGQAEWKILSASSEEPDTGFAHFAIDGKPDTYWHTSYTNGLPGFPHSIAVDMGTRMK
FTGFIYTPRMDKDKGLISQYAFSISDDGKNWKEVKKGQFTYHYIRKDPAVQRIDFGKPVE
ARYFKLDAKSPVRSGEQSATVAELNIITQ

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	47	938	1.2e-209	0.964095744680851
CBM32	1154	1284	4e-22	0.9112903225806451

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	0.0	27	1059	4	1002	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	0.0	26	1030	16	999	beta-galactosidase.
COG3250	LacZ	4.58e-149	53	936	14	803	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	2.56e-92	362	655	1	302	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
smart01038	Bgal_small_N	1.19e-63	784	1054	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWO96055.1	0.0	1	1289	1	1289
QWO91163.1	0.0	1	1289	1	1289
QWO93481.1	0.0	1	1289	1	1289
QWP70818.1	0.0	1	1289	1	1289
QWO86174.1	0.0	1	1289	1	1289

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	9.54e-200	26	1052	3	977	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	9.81e-200	26	1052	4	978	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3DEC_A	1.63e-176	23	1041	3	981	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3BGA_A	6.57e-174	25	1056	8	1000	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	6.20e-161	19	1042	8	1008	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56307	5.37e-199	26	1052	4	978	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
O52847	6.54e-180	25	1030	18	1005	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q32JB6	2.40e-161	11	1042	1	1009	Beta-galactosidase OS=Shigella dysenteriae serotype 1 (strain Sd197) OX=300267 GN=lacZ PE=3 SV=2
Q3Z583	1.28e-160	11	1042	1	1009	Beta-galactosidase OS=Shigella sonnei (strain Ss046) OX=300269 GN=lacZ PE=3 SV=1
A7ZI91	2.49e-160	11	1042	1	1009	Beta-galactosidase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) OX=331111 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.004490	0.993698	0.000856	0.000381	0.000298	0.000250

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000870_02178.