CAZyme Information: MGYG000000889_01018

Basic Information

• Species: CAG-279 sp900541555
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-279; CAG-279 sp900541555
• CAZyme ID: MGYG000000889_01018
• CAZy Family: GH9
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
840		94356.99	4.945

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000889	2172459	MAG	Denmark	Europe

• Gene Location: Start: 11161; End: 13683 Strand: +

Full Sequence      

MVIALKRVFFTVCLALPLSMPAFGESWIRINQLGYLPKSVKTAVFISEEKTDVGEFELVE
ALTMRPVAKFQTVANTGKYGTMESTCRLNFTSFEQQGSYCLKVGDTYSPAFRIGTDVYDG
TADFVLNYMRQQRCGYNPFLRDSCHRYDGYIEYHSTKTGQHLDVRGGWHDAGDYLQYTTT
SANAIYQMMLAYEANPEAFGDAYDAMGRKGANGIPDIVDEIKWGLDWLDRMNPEPGELYN
QIADDRDHASFRLPTSDAVDYGYGAGTGRPVYFCSGEPQQRGKFMNATTGVASTAGKFAS
CFAMGSNILADYYPEFAAKIREKAIPAYQEGVKKPGVCQTASVVSPYIYEEANWVDDMEL
GAAELYRLTGDSKYLQDAIEYGRREPITPWMGADSARHYQWYPFMNVGHYRVAAMGDARV
MAEFARNLRSGISRTYEKAKENPYLFGIPAIWCSNNLTTAMVTQCMLYRELTGDSTYVEM
EAALRDWLFGCNPWGTSMVVELPYGGDYPSQPHSAYVSEHVGNATGGLVDGPVYASIFES
LRGVNLDGLPWQKGEPYERFQPGDMVYHDAIGDYSTNEPTMDGTASMTYFLSSLQREGMM
QADRRSAAKRNEKISGGIVRTDTKEKTIALAFSAHTDAEGADKVIRTLDKYGINANFFFT
GYLYEHGENVVKRLLERGDYLGSHSYGHLQYIDVKRPDTTLVSRDEFTADLRKSYEAMGR
YGITPEKARYFLPPFEEYNATVASWANMLGLTVVNYTPGTGSAMDYTTPDLPYYRSSEEI
YRHIMDEEEKNGLNGHILLLHLGTDPARTDKFFDNCLDRLLADLLGKGYRFVLLEEAIGR

Enzyme Prediction     

No EC number prediction in MGYG000000889_01018.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH9	118	591	5.1e-80	0.992822966507177
CE4	621	755	6.7e-16	0.9384615384615385

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00759	Glyco_hydro_9	5.67e-73	126	591	7	374	Glycosyl hydrolase family 9.
cd10917	CE4_NodB_like_6s_7s	6.21e-31	626	824	1	170	Catalytic NodB homology domain of rhizobial NodB-like proteins. This family belongs to the large and functionally diverse carbohydrate esterase 4 (CE4) superfamily, whose members show strong sequence similarity with some variability due to their distinct carbohydrate substrates. It includes many rhizobial NodB chitooligosaccharide N-deacetylase (EC 3.5.1.-)-like proteins, mainly from bacteria and eukaryotes, such as chitin deacetylases (EC 3.5.1.41), bacterial peptidoglycan N-acetylglucosamine deacetylases (EC 3.5.1.-), and acetylxylan esterases (EC 3.1.1.72), which catalyze the N- or O-deacetylation of substrates such as acetylated chitin, peptidoglycan, and acetylated xylan. All members of this family contain a catalytic NodB homology domain with the same overall topology and a deformed (beta/alpha)8 barrel fold with 6- or 7 strands. Their catalytic activity is dependent on the presence of a divalent cation, preferably cobalt or zinc, and they employ a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. Several family members show diversity both in metal ion specificities and in the residues that coordinate the metal.
COG0726	CDA1	2.19e-23	567	838	6	257	Peptidoglycan/xylan/chitin deacetylase, PgdA/CDA1 family [Carbohydrate transport and metabolism, Cell wall/membrane/envelope biogenesis].
cd02850	E_set_Cellulase_N	3.29e-21	27	113	1	86	N-terminal Early set domain associated with the catalytic domain of cellulase. E or "early" set domains are associated with the catalytic domain of cellulases at the N-terminal end. Cellulases are O-glycosyl hydrolases (GHs) that hydrolyze beta 1-4 glucosidic bonds in cellulose. They are usually categorized into either exoglucanases, which sequentially release terminal sugar units from the cellulose chain, or endoglucanases, which also attack the chain internally. The N-terminal domain of cellulase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.
cd10950	CE4_BsYlxY_like	5.90e-21	621	838	1	188	Putative catalytic NodB homology domain of uncharacterized protein YlxY from Bacillus subtilis and its bacterial homologs. The Bacillus subtilis genome contains six polysaccharide deacetylase gene homologs: pdaA, pdaB (previously known as ybaN), yheN, yjeA, yxkH and ylxY. This family is represented by Bacillus subtilis putative polysaccharide deacetylase BsYlxY, encoded by the ylxY gene, which is a member of the carbohydrate esterase 4 (CE4) superfamily. Although its biological function still remains unknown, BsYlxY shows high sequence homology to the catalytic domain of Bacillus subtilis pdaB gene encoding a putative polysaccharide deacetylase (BsPdaB), which is essential for the maintenance of spores after the late stage of sporulation and is highly conserved in spore-forming bacteria. However, disruption of the ylxY gene in B. subtilis did not cause any sporulation defect. Moreover, the Asp residue in the classical His-His-Asp zinc-binding motif of CE4 esterases is mutated to a Val residue in this family. Other catalytically relevant residues of CE4 esterases are also not conserved, which suggest that members of this family may be inactive.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT66756.1	0.0	11	839	9	835
QUT35459.1	0.0	11	839	9	835
QUT98069.1	0.0	11	839	9	835
QBJ19309.1	0.0	11	839	9	835
QPH59586.1	0.0	11	839	9	835

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3X17_A	7.44e-38	28	589	18	551	Crystalstructure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium],3X17_B Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium]
5U2O_A	4.61e-33	36	592	2	537	Crystalstructure of Zn-binding triple mutant of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
5U0H_A	1.14e-29	36	592	2	537	Crystalstructure of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
4CJ0_A	7.29e-24	25	592	27	545	ChainA, ENDOGLUCANASE D [Acetivibrio thermocellus],4CJ1_A Chain A, ENDOGLUCANASE D [Acetivibrio thermocellus]
1CLC_A	7.70e-24	25	592	41	559	ChainA, ENDOGLUCANASE CELD; EC: 3.2.1.4 [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P23658	6.57e-26	28	594	4	545	Cellodextrinase OS=Butyrivibrio fibrisolvens OX=831 GN=ced1 PE=1 SV=1
P0C2S4	3.99e-23	25	592	27	545	Endoglucanase D (Fragment) OS=Acetivibrio thermocellus OX=1515 GN=celD PE=1 SV=1
A3DDN1	4.37e-23	25	592	51	569	Endoglucanase D OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celD PE=1 SV=1
P10476	2.95e-19	29	591	38	595	Endoglucanase A OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=celA PE=3 SV=2
A3DCH1	8.38e-19	28	516	214	740	Cellulose 1,4-beta-cellobiosidase OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celK PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000563	0.998660	0.000256	0.000179	0.000162	0.000151

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000889_01018.