CAZyme Information: MGYG000000902_00298

Basic Information

• Species: Clostridium neonatale
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium neonatale
• CAZyme ID: MGYG000000902_00298
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
952	MGYG000000902_2|CGC3	109323.41	5.5919

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000902	4770522	MAG	China	Asia

• Gene Location: Start: 95123; End: 97981 Strand: -

Full Sequence      

MLYYKSKIAITAEGKACDIFIDRYGNDYDGLKLVAKSFTEDITMITGIKPKVVTDVKELK
KSAIIAGSIGNNIIIDTLIINKRIDVSDIKERRECYKIQVVENPYKGIEKAIIIVGSDKR
GTMYGIYYISELIGVSPWSYFGDVVPEKKSELIFDENDLNFTSKEPSVKYRGFFLNDEWP
SLGSWVTNSFGDFNEEFYEKIFQLILRLKGNFMWPAMWSAVFSENGKSNPLANAKLADTY
GIVMGTSHHESMFRSGEEWKKINNKYGDNTTWDFASNKEAITKFWEDGLKRNKNFESFIT
IGMRGEQDSALEGSEEENIELLKEIIITQKDLLKKHGLEKERQVLTIYKEVEKYWYGTDK
AEGLRNWDVLDDVTIMLADDNFANVRTLPIEKEASRKAGWGMYYHFDYHGGPYSYEWVNV
TPLEKIWEQMSMAYDYGVRDIWIVNVGDLKPMEFPVSYFLDLAYSFETWGTNGINKIRKY
TKKWCMQQFGKVVGQDLINGIESILTDYTKMNGSRKPEVTSASTYSYINYNEAKRVLKKV
IDLENRSKKYYDQMPDYLKDAYYQLIYYPAVASANVVKMQIYAGLNSIYYKRKSCLANLY
AQLVEECKVKDTEMQNYYNNIMSNGKWKGMMSSPHIGYTQWCPDGWKYPEVNNVSLQNES
IMIVDVEGCEKGYSGGTVDLPSFTNLNKENYDITISNGGKIEFEYEVKTSKDWINVDNMH
GSISNGKTINISIDWSKVSKTLAGYITISGAGKIVKINVTAEVIDTYDLPKMTFIETHDV
VSIEAEHVSNNIEKAKASWKVIENYGRTLSSVKMFPTTVSFEKVEDAPYLEYLLKVNMDS
EYILTTYIAPTNNLREKSRLKYAVAFDGEKETIADAFLDDFIAGSYTNDSWCEAVLNNIH
ITTTKHRLTKGIHKLRFYGMDAGLVLQKLVLSKEKLPYSYFGPEESFYVAHK

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	7	929	2.4e-223	0.9956958393113343

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	8.93e-174	164	519	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	1.04e-58	773	945	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam19190	BACON_2	3.51e-04	692	763	19	90	Viral BACON domain. This family represents a distinct class of BACON domains found in crAss-like phages, the most common viral family in the human gut, in which they are found in tail fiber genes. This suggests they may play a role in phage-host interactions.
cd14948	BACON	0.003	685	760	3	81	Bacteroidetes-Associated Carbohydrate-binding (putative) Often N-terminal (BACON) domain. The BACON domain is found in diverse domain architectures and accociated with a wide variety of domains, including carbohydrate-active enzymes and proteases. It was named for its suggested function of carbohydrate binding; the latter was inferred from domain architectures, sequence conservation, and phyletic distribution. However, recent experimental data suggest that its primary function in Bacteroides ovatus endo-xyloglucanase BoGH5A is to distance the catalytic module from the cell surface and confer additional mobility to the catalytic domain for attack of the polysaccharide. No evidence for a direct role in carbohydrate binding could be found in that case. The large majority of BACON domains are found in Bacteroidetes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGF58615.1	0.0	8	948	4	944
AQR97306.1	0.0	8	948	4	944
APC42637.1	0.0	19	949	2	932
AEY65938.1	0.0	10	948	9	947
AIQ35136.1	0.0	7	948	5	946

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6NPS_A	1.08e-240	30	949	31	966	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
4ZMH_A	2.57e-199	8	946	14	933	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
7PUG_A	1.27e-149	7	641	16	635	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	1.33e-148	7	641	15	634	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
4C90_A	1.64e-146	10	641	46	650	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000902_00298.