CAZyme Information: MGYG000000902_02874

Basic Information

• Species: Clostridium neonatale
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium neonatale
• CAZyme ID: MGYG000000902_02874
• CAZy Family: GH73
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
734	MGYG000000902_44|CGC1	82812	4.3836

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000902	4770522	MAG	China	Asia

• Gene Location: Start: 23984; End: 26188 Strand: -

Full Sequence      

MKIEKKFIILTIIFSLILIMSQYQYTYAKDLNDLLIISDTNITANDAEKWAKSKGATETF
IDLADLYFEYSEDCGNVNPAIAYVQAAKETGFGNFGGILDESYHNPCGLKISSGGGNNSA
SSHQVFENWDEGVQAHLDHLALYAGADGYPKRDTYDPRHFITLKGKAKTVNDLTGNWATS
NIYGEEINKLYRDLLKYSDASINDEEIENNNDNKYSVPYPAEPESIPEGLDASSVIPLTI
TDDKEDTTPNEDSNIGFKTENDSLVYYRSDYTKVIGWLKTDDKWYFMKDDGTALTGWLND
YGRWYYFSESHEMIKGWEKINDKWYYFKTSGEMTTGPLYDGTNLYYFDSSGTMVTGTGWK
DFNGNFYHISSNGVLKTGWIKDNNIWYYLQGDGTMVTRYKVINSKIYGFDKSGKMITGWI
KDNNNWYFFNSDGSIATGWLKDSNSKRYYLYNTGAMAKGWINLNDSWYYLNNSGDMQTGW
LNFNEDSYYLDPSTGKLLTDTVIDNIQIFSDGTTKNSDDKSNISSYSKKNNDEVIIVIDA
GHYNNGDKGSERTFNGITYSETDLNLELAKKLKDNLEEKGYTTIMTREDGDDSSVSVTSS
LANRVNIANNAGADLFISIHHNTTADYESDIKGVEVYYSSTRQDDEFGGNYNYDRVLNSK
NLATSVSKNIASSVSTNNRGAKDNNLFVCRNTNMPAILIETGFISNIEEAKRCADKDEQE
NVAEAIADAVSELY

Enzyme Prediction     

No EC number prediction in MGYG000000902_02874.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02696	MurNAc-LAA	7.58e-58	535	730	1	171	N-acetylmuramoyl-L-alanine amidase or MurNAc-LAA (also known as peptidoglycan aminohydrolase, NAMLA amidase, NAMLAA, Amidase 3, and peptidoglycan amidase; EC 3.5.1.28) is an autolysin that hydrolyzes the amide bond between N-acetylmuramoyl and L-amino acids in certain cell wall glycopeptides. These proteins are Zn-dependent peptidases with highly conserved residues involved in cation co-ordination. MurNAc-LAA in this family is one of several peptidoglycan hydrolases (PGHs) found in bacterial and bacteriophage or prophage genomes that are involved in the degradation of the peptidoglycan. In Escherichia coli, there are five MurNAc-LAAs present: AmiA, AmiB, AmiC and AmiD that are periplasmic, and AmpD that is cytoplasmic. Three of these (AmiA, AmiB and AmiC) belong to this family, the other two (AmiD and AmpD) do not. E. coli AmiA, AmiB and AmiC play an important role in cleaving the septum to release daughter cells after cell division. In general, bacterial MurNAc-LAAs are members of the bacterial autolytic system and carry a signal peptide in their N-termini that allows their transport across the cytoplasmic membrane. However, the bacteriophage MurNAc-LAAs are endolysins since these phage-encoded enzymes break down bacterial peptidoglycan at the terminal stage of the phage reproduction cycle. As opposed to autolysins, almost all endolysins have no signal peptides and their translocation through the cytoplasmic membrane is thought to proceed with the help of phage-encoded holin proteins. The amidase catalytic module is fused to another functional module (cell wall binding module or CWBM) either at the N- or C-terminus, which is responsible for high affinity binding of the protein to the cell wall.
NF033930	pneumo_PspA	7.45e-56	256	474	444	640	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033930	pneumo_PspA	2.54e-53	276	512	444	657	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
pfam01520	Amidase_3	5.52e-51	536	730	1	172	N-acetylmuramoyl-L-alanine amidase. This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls.
NF033838	PspC_subgroup_1	2.24e-49	272	488	483	674	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CUU50706.1	3.75e-127	1	731	1	753
AIY80458.1	1.12e-123	21	730	23	732
APF24287.1	1.19e-123	3	734	7	765
ACD24287.1	8.62e-123	1	730	1	732
BBK78145.1	9.16e-123	3	734	7	765

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4RN7_A	3.14e-17	536	730	6	178	ChainA, N-acetylmuramoyl-L-alanine amidase [Clostridioides difficile 630]
1JWQ_A	2.56e-15	535	732	3	174	Structureof the catalytic domain of CwlV, N-acetylmuramoyl-L-alanine amidase from Bacillus(Paenibacillus) polymyxa var.colistinus [Paenibacillus polymyxa]
5EMI_A	4.84e-15	533	727	4	171	ChainA, Cell wall hydrolase/autolysin [Nostoc punctiforme PCC 73102]
7RAG_B	3.33e-12	536	726	19	201	ChainB, Germination-specific N-acetylmuramoyl-L-alanine amidase, Autolysin [Clostridioides difficile]
5J72_A	4.56e-10	536	706	454	609	ChainA, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630],5J72_B Chain B, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O51481	6.25e-18	77	201	84	202	Uncharacterized protein BB_0531 OS=Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31) OX=224326 GN=BB_0531 PE=3 SV=1
Q02114	9.43e-16	536	734	322	495	N-acetylmuramoyl-L-alanine amidase LytC OS=Bacillus subtilis (strain 168) OX=224308 GN=lytC PE=1 SV=1
P54525	2.96e-15	536	734	32	204	Uncharacterized protein YqiI OS=Bacillus subtilis (strain 168) OX=224308 GN=yqiI PE=3 SV=3
Q9RMZ0	4.76e-12	519	734	316	520	Uncharacterized cell wall amidase pXO2-42/BXB0045/GBAA_pXO2_0045 OS=Bacillus anthracis OX=1392 GN=pXO2-42 PE=3 SV=2
O48471	7.40e-11	535	733	4	180	Endolysin OS=Bacillus phage SPP1 OX=10724 GN=25 PE=4 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.006971	0.991478	0.000781	0.000263	0.000234	0.000241

TMHMM  Annotations     

start	end
7	26