CAZyme Information: MGYG000000906_01865

Basic Information

• Species: Phocaeicola sp900552075
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola sp900552075
• CAZyme ID: MGYG000000906_01865
• CAZy Family: GH97
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
908	MGYG000000906_13|CGC1	103514.33	5.6097

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000906	2359165	MAG	China	Asia

• Gene Location: Start: 11288; End: 14014 Strand: -

Full Sequence      

MKNTSLFLLGAVLWLFGCLSSMAASNKIYVVQSPDKHLKVEVSSGANGLTYCIYHKDSLI
VVDSKLGLIQEGKTSASVGGEKVISSKTKKMIQDIDAPFYRFKSFQVTCNEMNLKLKGGC
GAYFRVYNDGVAYRFYTSSKEDLIIKNEIAEFRFAGDYTAYLPYSTNKEKPMAMAFQNTY
EVKPLSEAPQELAFLPVTVDCKQVKVTLLESDLEAYPGMFVQPDGKQALKGVFAPYPKKT
DFYPWRKQEYVTEAEDYIAHVKGNRTYPWRILAITEKDTDMPVNNLVYALASPNRIGDCS
WVKPGKVAWDWWNDWNLKGVPFKAGINMDTYKYYIDFASRNGLEYVVLDEGWYDPKSGDM
LKTIPELDLPELVRYGKSKGVELVLWTVFNVLDSQLDEACRKYSEMGIKGFKVDFLDRDD
QTAVEMIYRIAAKAAEYKLMLDYHGIYKPTGLNRTYPNVVNYESVFGMEEMKWSEVEKNM
PLYDVTFPYIRLMAGYVDYTPGAMQNLSKRDFQPMYSTPASMGTRCHQLAAYIVHDSPFT
MLCDAPTNYQKEQECVDFISSIPMEVDSTFIYSGKLGESIVTVRKKDINWYIGGMTNWDE
RDITLDFSFLGKGEKYRCTLFKDGINASHQAEDYVKQTFIVDANTKLPIHLASGGGFALK
LERTFVAEVKPSAVPEGKGIPAFYKKYLAVDGLYIVSSEHVRDEALEKAYEIVSLMTAKR
PDVKKEMVSKGCHVMIIGEHEEVCDLPEYAHICNTPENIAFWNKRARGFGGAPEDDFSAS
CGEENVLALPGDRYVGENILIHEFAHLFHTIGIVGVEPDFNDRLEKCMQHAIAKGLWKDT
YALSNKEEYFAECVQSFFNCNRYSETPNKVHNSMNRRTKLKSYDPEMYQLLKEYFYEIEI
PILNEVHL

Enzyme Prediction     

No EC number prediction in MGYG000000906_01865.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	19	661	5.9e-189	0.9904912836767037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	1.70e-135	298	563	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	1.93e-55	33	293	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	2.38e-39	567	661	2	96	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.
cd20169	Peptidase_M90_mtfA	4.74e-08	787	895	104	208	Mlc titration factor A (MtfA) is a zinc metallopeptidase (M90 peptidase). This subfamily includes the Mlc Titration Factor A (MtfA; also known as YeeI or DgsA anti-repressor MtfA) which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). It can cleave synthetic substrates of both carboxypeptidases and aminopeptidases, with strongest activity towards the latter. Its biologically relevant substrate has yet to be identified. Although it interacts with the transcription repressor Mlc, it does not cleave it. However, Mlc seems to activate the peptidase activity of MtfA. MtfA is related to the catalytic domain of the anthrax lethal factor which is a zinc-dependent metalloprotease, targeting mitogen-activated protein kinase kinases (MAPKKs), and resulting in apoptosis, as well as the Mop (modulation of pathogenesis) protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.
cd20170	Peptidase_M90-like	2.31e-06	798	892	117	206	uncharacterized M90 peptidase family-like proteins. This subfamily contains uncharacterized M90 peptidase-like domains, similar to the Mlc Titration Factor A (MtfA) peptidase from Escherichia coli, also known as the YeeI gene product, which is involved in the control of the glucose-phosphotransferase sensory and regulatory system by inactivation of the repressor Mlc (making large colonies). E. coli MtfA has been shown to have aminopeptidase activity with the presence of a single zinc ion in the active site ligated by two histidines in an HEXXH motif. MtfA is related to the catalytic domain of the anthrax lethal factor and the Mop protein involved in the virulence of Vibrio cholerae; although sequence similarity is low, conservation is observed in the overall structure as well as in the residues around the active site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABR38815.1	0.0	20	907	21	906
QQY38934.1	0.0	20	907	21	906
QEW34704.1	0.0	20	907	21	906
QJR66042.1	0.0	2	907	3	906
QJR70731.1	0.0	2	907	3	906

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	1.04e-197	33	662	6	641	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	1.86e-196	33	662	20	655	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQC_A	7.30e-58	31	662	3	659	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQ4_A	1.84e-57	31	662	3	659	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	4.64e-57	31	662	3	659	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	7.21e-198	18	662	13	662	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
D7CFN7	1.77e-45	34	658	46	617	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
G8JZS4	2.30e-45	16	680	12	735	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000570	0.495642	0.503146	0.000228	0.000207	0.000189

TMHMM  Annotations     

start	end
5	24