CAZyme Information: MGYG000000915_01004

Basic Information

• Species: Clostridium nigeriense
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium nigeriense
• CAZyme ID: MGYG000000915_01004
• CAZy Family: CBM40
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
928	MGYG000000915_2|CGC5	102117.74	6.3767

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000915	3503945	MAG	China	Asia

• Gene Location: Start: 401890; End: 404676 Strand: -

Full Sequence      

MYNLIKRSISTIVLTTLAITSLPTHSASSQTTAEYSAKKYNIESTKNIATEPFNLVQSLS
APMLEKENVEILSGTVFDLTKEAAATSILSMTEGTIIIKFKSNSNNQYQSLFSVSNSTTG
NQDRHFHIYMTPDGSLGMELRNTDADFKYTMNVPNVLSVGKENIIAFKGDKISKEYKLFA
NGKLVSTISKNNFKFFNDILGADNISLGGTIRQKNIAYPFVGTINKLSIYNLNLTDNELI
EATNNSSIPSGLIIEKNNINITEGKGYDLSQEVGAEYVKALEKGTILVSYKSTSSNSVQS
LISIGNGTSGNRDRHFHIYITNTGEVGMELRNTDSVLKYTLGRPAAVRSIYKNNLVFNTI
ALKADPSTKQYKIFANGELLATLSVDVYKFINDITGVNNIMLGGTLRDGVIAYPFGGTIG
SVKIYNEILTDDVLKNETGITSYGKNIFYAGDSTKSNYFRIPSLLSLSSGTVVSAADARY
GGTHDSKSNIDIAFSKSKDGGITWLNPTIPLQFNDYVAKNIEWPRDSVGKNVQIQGSASF
IDPVLLEDKETKRLFIFADAMPAGIGSSNASTGSGYKDIAGKKYMKLRWHQDGSSTYNYS
IRENGVIYNDVTNLPTEYKIDGEYNLYKNGVALFCKQYDYNFSGTTLLETATNVDVNMNV
FYKDSLFKVFPTTYLAMKYSDDEGETWSNLNIVSSFKPENSKFLVLGPGVGKQISKGQYK
GRLIVPVYSSSYAELGFMYSDDHGSSWNYVAADNRNTGSTAEAQIVEMPDGSLKSYLRTG
SGVIAEVTSINGGETWSERVTVPNMHTTSYGTQLSVINYSGLIDGKEAIILSAPDSTSAR
GNGKIWIGLINDTGNSGVNKYTVEWKYCYSVDSANMGYSYSCLTELPNGDIGLLYEKYDS
WSRNELHLKNILKYETFSINELKQPISN

Enzyme Prediction     

EC	4.2.2.15	3.2.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH33	449	904	4.5e-79	0.935672514619883
CBM40	257	440	3.2e-52	0.9776536312849162
CBM40	67	243	8e-43	0.9720670391061452

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4409	NanH	7.08e-121	264	923	83	727	Neuraminidase (sialidase) [Carbohydrate transport and metabolism, Cell wall/membrane/envelope biogenesis].
cd15482	Sialidase_non-viral	1.02e-60	447	911	3	338	Non-viral sialidases. Sialidases or neuraminidases function to bind and hydrolyze terminal sialic acid residues from various glycoconjugates, they play vital roles in pathogenesis, bacterial nutrition and cellular interactions. They have a six-bladed, beta-propeller fold with the non-viral sialidases containing 2-5 Asp-box motifs (most commonly Ser/Thr-X-Asp-[X]-Gly-X-Thr- Trp/Phe). This CD includes eubacterial and eukaryotic sialidases.
pfam02973	Sialidase	1.78e-23	259	441	14	188	Sialidase, N-terminal domain.
pfam02973	Sialidase	4.76e-21	65	239	8	181	Sialidase, N-terminal domain.
pfam13088	BNR_2	3.48e-17	680	893	87	280	BNR repeat-like domain. This family of proteins contains BNR-like repeats suggesting these proteins may act as sialidases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAA69951.1	0.0	1	928	1	773
ANU76745.2	0.0	253	922	38	707
QJU17595.1	0.0	253	922	38	707
ASU31741.1	0.0	253	922	38	707
QQQ95451.1	0.0	253	922	38	707

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1SLI_A	0.0	248	922	1	675	LeechIntramolecular Trans-Sialidase Complexed With Dana [Macrobdella decora],1SLL_A Sialidase L From Leech Macrobdella Decora [Macrobdella decora],2SLI_A Leech Intramolecular Trans-Sialidase Complexed With 2,7- Anhydro-Neu5ac, The Reaction Product [Macrobdella decora],3SLI_A Leech Intramolecular Trans-Sialidase Complexed With 2,7- Anhydro-Neu5ac Prepared By Soaking With 3'-Sialyllactose [Macrobdella decora],4SLI_A Leech Intramolecular Trans-Sialidase Complexed With 2- Propenyl-Neu5ac, An Inactive Substrate Analogue [Macrobdella decora]
4X6K_A	6.33e-274	444	925	4	486	Crystalstructure of the intramolecular trans-sialidase from Ruminococcus gnavus in complex with Siastatin B [[Ruminococcus] gnavus CC55_001C]
4X47_A	7.07e-274	444	925	7	489	Crystalstructure of the intramolecular trans-sialidase from Ruminococcus gnavus in complex with Neu5Ac2en [[Ruminococcus] gnavus ATCC 29149],4X49_A Crystal structure of the intramolecular trans-sialidase from Ruminococcus gnavus in complex with oseltamivir carboxylate [[Ruminococcus] gnavus ATCC 29149],4X4A_A Crystal structure of the intramolecular trans-sialidase from Ruminococcus gnavus in complex with 2,7-Anhydro-Neu5Ac [[Ruminococcus] gnavus ATCC 29149]
4YZ1_A	2.41e-181	249	924	19	676	CrystalStructure of Streptococcus pneumoniae NanC, apo structure. [Streptococcus pneumoniae TIGR4],4YZ1_B Crystal Structure of Streptococcus pneumoniae NanC, apo structure. [Streptococcus pneumoniae TIGR4],4YZ2_A Crystal Structure of Streptococcus pneumoniae NanC, in complex with 2-deoxy-2,3-didehydro-N-acetylneuraminic acid. [Streptococcus pneumoniae],4YZ2_B Crystal Structure of Streptococcus pneumoniae NanC, in complex with 2-deoxy-2,3-didehydro-N-acetylneuraminic acid. [Streptococcus pneumoniae],4YZ3_A Crystal Structure of Streptococcus pneumoniae NanC, in complex with Oseltamivir. [Streptococcus pneumoniae TIGR4],4YZ3_B Crystal Structure of Streptococcus pneumoniae NanC, in complex with Oseltamivir. [Streptococcus pneumoniae TIGR4],4YZ4_A Crystal Structure of Streptococcus pneumoniae NanC, in complex with N-Acetylneuraminic acid. [Streptococcus pneumoniae],4YZ4_B Crystal Structure of Streptococcus pneumoniae NanC, in complex with N-Acetylneuraminic acid. [Streptococcus pneumoniae],4YZ5_A Crystal Structure of Streptococcus pneumoniae NanC, in complex with 3-Sialyllactose [Streptococcus pneumoniae],4YZ5_B Crystal Structure of Streptococcus pneumoniae NanC, in complex with 3-Sialyllactose [Streptococcus pneumoniae]
4YW1_A	3.78e-181	253	926	4	659	ChainA, Neuraminidase C [Streptococcus pneumoniae TIGR4],4YW1_B Chain B, Neuraminidase C [Streptococcus pneumoniae TIGR4],4YW2_A Chain A, Neuraminidase C [Streptococcus pneumoniae TIGR4],4YW2_B Chain B, Neuraminidase C [Streptococcus pneumoniae TIGR4],4YW3_A Chain A, Neuraminidase C [Streptococcus pneumoniae TIGR4],4YW3_B Chain B, Neuraminidase C [Streptococcus pneumoniae TIGR4],4YW4_A Streptococcus pneumoniae sialidase NanC [Streptococcus pneumoniae],4YW4_B Streptococcus pneumoniae sialidase NanC [Streptococcus pneumoniae],4YW5_A Chain A, Neuraminidase C [Streptococcus pneumoniae TIGR4],4YW5_B Chain B, Neuraminidase C [Streptococcus pneumoniae TIGR4],5F9T_A Chain A, Neuraminidase C [Streptococcus pneumoniae TIGR4],5F9T_B Chain B, Neuraminidase C [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q27701	0.0	246	922	79	755	Anhydrosialidase OS=Macrobdella decora OX=6405 PE=1 SV=1
Q54727	3.98e-162	282	922	68	695	Sialidase B OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=nanB PE=1 SV=2
P29767	8.45e-99	175	925	125	853	Sialidase OS=Clostridium septicum OX=1504 PE=3 SV=1
P62575	1.72e-47	253	896	121	768	Sialidase A OS=Streptococcus pneumoniae OX=1313 GN=nanA PE=1 SV=1
P62576	1.72e-47	253	896	121	768	Sialidase A OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=nanA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000878	0.997619	0.000848	0.000232	0.000205	0.000190

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000915_01004.