CAZyme Information: MGYG000000920_01146

Basic Information

• Species: Gemmiger sp900548355
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger; Gemmiger sp900548355
• CAZyme ID: MGYG000000920_01146
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
671		76263.4	6.5078

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000920	2473301	MAG	China	Asia

• Gene Location: Start: 53472; End: 55487 Strand: -

Full Sequence      

MQATPEPKPSDLPIYLFKQGNNMEAYRYFGAHLCEQEGQAGVVFRVWAPHAQAVSVVGDF
NNWTPGQNPMQKVDDAVWEAFVPGMKQYDVYKFCVTTPAGEQVYKADPYAFHAETRPSNG
SKVYDISGYAWGDAAWEEAQKKRDVINGPMLIYELHAGSWKMKADGVPYNYSELADELIP
YIQEMGYTHVELLPITEYPFDGSWGYQVTGYFAPTSRYGTPHDFMAFVDKLHRAGIGVIL
DWVPAHFPKDGYGLYMFDGAPCYEDPNPRRGEHKEWGTMVFNYGMPEVQSFLISSALYWV
EQYHVDGLRVDAVASMLYLDYNRRDGEWEANKNGGKENLEAIAFLQKCNSTVLGRYPYKM
MIAEESTAWPLVTKPAEDGGLGFNFKWNMGWMNDMLSYMKTDPLFRAGNHGKVTFSFFYA
FSENFVLPISHDEVVHGKCSLINKMPGDYDQKFANLRTFYGYMLAHPGKKLLFMGQEFGQ
FIEWDEKKPLDWMLLGYDKHHQLQSYVKALNRFYLENPAMWEVDYSWEGFQWIVPDDNQQ
SVIAFLRRDKKGKMIMVVCNFNPVLREGYQMGVPTSGSYKELLNSDDAQYGGGGVTNGTV
RSKKGAMHGFDQHVSLTLPPLSTLYFSVPAPRKRTTKPATAKKPARSAAQKTAQGARARK
PKSQPSENAAE

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	180	480	3.8e-158	0.9966777408637874
CBM48	29	113	6.9e-22	0.8947368421052632

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	7	635	5	633	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	29	638	27	633	glycogen branching enzyme; Provisional
PRK14705	PRK14705	0.0	7	626	601	1220	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	7	628	4	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK05402	PRK05402	0.0	7	630	98	725	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AXA81819.1	0.0	9	636	12	641
AXB29737.1	0.0	10	651	13	656
CBL01646.1	0.0	10	649	13	654
CBK98932.1	0.0	9	636	12	641
QIA41789.1	0.0	9	634	12	639

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	2.66e-230	3	624	123	769	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	3.77e-230	3	624	123	769	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	5.33e-230	3	624	123	769	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	7.55e-230	3	624	123	769	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	1.51e-229	3	624	123	769	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B8CVY1	5.37e-260	9	626	6	625	1,4-alpha-glucan branching enzyme GlgB OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=glgB PE=3 SV=1
Q1AZ86	5.09e-251	7	625	97	716	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q8DLB8	8.42e-243	7	624	104	746	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q7NL20	1.03e-240	10	626	104	726	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
Q0I8Y2	4.92e-239	11	626	108	748	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain CC9311) OX=64471 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000074	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000920_01146.