CAZyme Information: MGYG000000922_01530

Basic Information

• Species: UMGS416 sp900542005
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; UMGS416; UMGS416; UMGS416 sp900542005
• CAZyme ID: MGYG000000922_01530
• CAZy Family: GH116
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
852		95176.53	5.3893

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000922	2901170	MAG	China	Asia

• Gene Location: Start: 23287; End: 25845 Strand: -

Full Sequence      

MQADFQYRGAKLATLSMPLGGIGTGCVGLAGNGHLVDWEIFNRPNKQSLNGFTHLAVKAE
GKGGLLDARVLQGPPPAPYTGEGDGVFSGFGFGPSRYTLGGAPCFSSNEMEAGFPLARIR
FQEAGFPGRAALSAFNPFIPREDRDSSFPAAFFQVELTNTTDEPLRYTAAFTLANPHKGG
HDHRCGREQGISYCLLGRQWRDETDLLDGEVAVATDAQESGWQPYWYRGKWFDGLNMFWH
DFARPGPLPQRSYPPGGEGEDAAVLTASALAAPGETVRLRFVLSWYYPNQCRYWGDPPAR
HTQWHNYYATQWPGALAVAREALRRYDELYEKTRVFHDALFSCTLPRETLDAVSANLSIL
KSPTVLRLEDGSFYGWEGCMARQGSCEGSCTHVWNYAYALPFLFPALERSMRELDYTYNL
KADGGMPFRLQLPLGAPPLQFRPCVDGQMGGVMKMYREWKISGDTAWLRRWWPAVKQSLA
YAWSPENPDRWDPEQCGLITGRQHHTLDMELFGPNAWLTGFYLGALKAAGEMARALDDPD
AAQYEAIFERGRARADAELFNGSYYVQRVDLSDRKLLEAFDQGPMDMTGERASETYWNQE
TGEMKYQIGEGCAIDQVVAQWHANLMGLGQIFDPEQTAKAMDSVYRYNFKPRLGEVFNPC
RLYGLADEAGTVICAYPPGAYKPAIAVPYAEETMHGFEYQAAIHLLQLGRLEEGFEMIAA
VRRRYDGARRNPFNEMECGSNYARSMASFAALAALSGFRYDMTRGMIGFSPQQTGDFSTL
WSLEPAWGTYCVCNGRAALSVLGGCLPLRQLALPGRRPVELRLQGQPVSWRMEGEMILLE
ADIQAGQRLEIL

Enzyme Prediction     

No EC number prediction in MGYG000000922_01530.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	374	750	9.1e-61	0.9862258953168044

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12215	Glyco_hydr_116N	1.35e-53	17	329	2	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.
pfam04685	DUF608	1.20e-36	382	746	2	357	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
COG4354	COG4354	9.20e-30	17	721	15	674	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].
COG3459	COG3459	2.75e-04	513	680	754	888	Cellobiose phosphorylase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCO21811.1	0.0	4	839	3	853
AEE95836.1	0.0	5	814	6	833
ANE45793.1	0.0	7	814	5	822
AZN43107.1	3.27e-314	6	813	4	818
QHW31016.1	7.03e-314	7	837	5	846

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FJS_A	2.67e-20	18	715	55	734	Bacterialbeta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5FJS_B Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2) [Thermoanaerobacterium xylanolyticum LX-11],5O0S_A Crystal structure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with unreacted beta Cyclophellitol Cyclosulfate probe ME711 [Thermoanaerobacterium xylanolyticum LX-11]
5BVU_A	2.71e-20	18	715	59	738	Crystalstructure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],5BX2_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with 2-deoxy-2-fluoroglucoside [Thermoanaerobacterium xylanolyticum LX-11],5BX3_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with deoxynojirimycin [Thermoanaerobacterium xylanolyticum LX-11],5BX4_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with Glucoimidazole [Thermoanaerobacterium xylanolyticum LX-11],5BX5_A Crystal structure of Thermoanaerobacterium xylanolyticum GH116 beta-glucosidase with glucose [Thermoanaerobacterium xylanolyticum LX-11],5NCX_A Crystal structure of Thermoanaerobacterium xylolyticum GH116 beta-glucosidase with an covalent inhibitor [Thermoanaerobacterium xylanolyticum LX-11],5OST_A Beta-glucosidase from Thermoanaerobacterium xylolyticum GH116 in complex with Gluco-1H-imidazole [Thermoanaerobacterium xylanolyticum LX-11]
5NPF_A	2.71e-20	18	715	58	737	Crystalstructure of txGH116 (beta-glucosidase from Thermoanaerobacterium xylolyticum) in complex with beta Cyclophellitol Cyclosulfate probe ME594 [Thermoanaerobacterium xylanolyticum]
7DKS_A	2.71e-20	18	715	59	738	ChainA, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKT_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKU_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKV_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11]
7DKW_A	2.71e-20	18	715	59	738	ChainA, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKW_B Chain B, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKX_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11],7DKY_A Chain A, beta-glucosidase [Thermoanaerobacterium xylanolyticum LX-11]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.961738	0.014716	0.023452	0.000027	0.000019	0.000047

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000922_01530.