CAZyme Information: MGYG000000924_01383

Basic Information

• Species: An172 sp002160515
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; An172; An172 sp002160515
• CAZyme ID: MGYG000000924_01383
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1628		178578.72	4.1237

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000924	2081481	MAG	China	Asia

• Gene Location: Start: 223; End: 5109 Strand: +

Full Sequence      

MKIGNLKWRKIASAALAGMILLSSVPAFSAQENEPLVSSVQENSESDALRLWYTAPSSKC
GASGEKDIWQQCTLPIGNGDIGANVYGEIVNERLTFNEKTLWTGGPSESRPDYNGGNLTQ
NGQNGETMKQIQQLFAEGKDAEASSLCNKLVGTSDGYGSYQSWGNIYFNHTGLNENNITG
YVRDLDLNNAIASVEFDSNGTHYTREYFISNPDNVLVAHFTAEGSDKLNMEITFPSNQDG
KTVAEGNSLYLAGEVSDNQLKYDSVLKAVTETGKVDASGDKLVVSGAQELTVYVSADTDY
KNEYPKYRTGETAEELHEKVNTTVENAAAKPYEEVKNTHVEDYSDLFGRVELDLGQSVSD
KTTDALLKAYNNGTATEEEQRQLEVMLFQYGRYLTIASSRENSELPSNLQGVWNNVNNPQ
WSSDYHMNVNLQMNYWPTYSTNLAECADPLIRYVDSLREPGRVTAAIYAGVVSEEGEENG
FMAHTQNTPFGWTCPGWAFSWGWSPAAVPWILQNCWEYYEYTGDYDYMVNNIYPMLREEA
MLYNSMLIKDEDGKYVSSPTYSPEQGPYTAGNTYEQSLIWQLFEDAITAGKLVGEDEEVI
AQWQEKQDNLKGPIEIGDDGQIKEWYIETSYNKDENGNTLGEGYGHRHLSHMLGLFPGDL
ITEDTPEWFEAAKVSMNMRTDSSTGWGMGQRINTWARLGDGNRAHKLITDLFKSGIYMNL
WDTHPPFQIDGNFGMTSGVSEMLVQSNAGYINLLPALPDEWADGSVSGLVARGNFEITMN
WENKSIQTATLLSNNGGEAIVQADRLSLGTVKDSDGNYVDFEVLKDNRISFETEAGKSYT
ISSIPAEEEKAESPANIKAYKLTDGQTEVLWDAVDAENAVYNVYRQINDGEWYLIGTDIS
DLSFKDTDAYDVLGTLSYKVSAVIDGEETKLSGAVSVMDMRNMAGMMDDQDSRIVYSGNW
GNWTGDADNYKGTIKFLETPNGSETISLTFCGTGIEVLTCKNHDRGTMRVDIDGVDMGTV
DTYSPTTQRQVKIFSKDDLEYGIHTITLTATGEHSAQSSKAKLEFDGFNVLDNSVAYPES
LKVVSSNGITTVSKADTQLQMKAYSMPEGTENTGVVWSVETKSGNTTAKIDENGLLTLDG
ENGVVTVKAAMKNNGNIAGTVDITVAIPTENDNYEIVEDSVNKANPNPAITWNGSWSTWA
GEPTRHHGGTKTDGSRAGSSFSYTFTGTGIELYAQKNLKYGKYTVVLDGEDAGEASLVGT
VQGGEDQQLVYSKLGLENKEHTITFTVAAVDGNTTVNMDYFKVYKPSEEIVDKSQLQEAI
EKYGDRTEAEYTQEAWAPFEAAMKAAVEGMNSSETTAEQVQTLADNLNKAGEELDKQEIV
APEFAEDAKAVVSGIESDKVTLIWDKAQCAVSYKVVVKDSEGAEVKTAETENSYLQINEL
NPNTEYVFEVYGVNRNGKASETPLTVKGTTAAPIDNEAPAQVTGLAATEISGANQAKITW
EKSTDPQGSLVTYEVYINGIKAAETQETEFIFDVIDGTSYSIRIVAVDEAGNKSLPAVYN
YATGSQSVKGDVNKDGKADVEDVTLIQKFIVELDLGEAIFDEKAADVNGDGIITVTDATE
IQIMLGRS

Enzyme Prediction     

EC	3.2.1.63

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	45	804	1.5e-255	0.9847645429362881

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	4.82e-66	51	302	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
cd14256	Dockerin_I	1.96e-13	1569	1622	1	53	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
pfam00404	Dockerin_1	4.27e-08	1570	1621	1	51	Dockerin type I repeat. The dockerin repeat is the binding partner of the cohesin domain pfam00963. The cohesin-dockerin interaction is the crucial interaction for complex formation in the cellulosome. The dockerin repeats, each bearing homology to the EF-hand calcium-binding loop bind calcium.
cd14255	Dockerin_III	6.38e-06	1570	1621	1	56	Type III dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. Two specific calcium-dependent interactions between cohesin and dockerin appear to be essential for cellulosome assembly, type I and type II. This subfamily represents the atypical type III dockerins and related domains.
cd00063	FN3	3.10e-05	1392	1470	9	93	Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHB24557.1	0.0	9	1586	10	1586
QEI32060.1	0.0	9	1586	10	1586
QRT30709.1	0.0	9	1586	10	1586
ASM69030.1	0.0	7	1562	5	1558
QNM13266.1	0.0	65	1389	70	1376

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	3.78e-241	36	842	2	895	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	2.92e-240	36	842	2	895	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	5.58e-240	36	842	1	894	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
2RDY_A	4.62e-132	48	842	2	790	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	1.29e-118	49	796	22	736	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	9.99e-96	74	781	67	792	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	1.72e-90	48	786	20	761	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	2.91e-77	43	786	16	780	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	1.26e-68	76	795	35	711	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000533	0.998615	0.000208	0.000251	0.000181	0.000161

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000924_01383.