CAZyme Information: MGYG000000932_01596

Basic Information

• Species: UMGS577 sp900543525
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; UMGS577; UMGS577 sp900543525
• CAZyme ID: MGYG000000932_01596
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1492		164044.74	4.6184

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000932	1983140	MAG	Germany	Europe

• Gene Location: Start: 2492; End: 6970 Strand: -

Full Sequence      

MKKMIAVLLALAVFCSLLPTWSAGLPAKAAARFPLFAEGKAADLYVSAADYPQIVRAAGD
LQKDVERVTAALPEVKTDTSALGQYAVIIGSVEKSPVIAQLMEQGLLDEAKALSGKFEAF
VIKMVENPLPGVKSALVIAGSDKRGTVYGIYDVSEQIGVSPYYWWADVTPKQQSELLLDG
GVKTEGEPSVKYRGIFINDERNLTEWSAQFASEETPGTPNAGTYEKVFELLLRLRLNTLW
PAMHNYSEAFNLREDENGVPINAKLADSYGIIMGASHCEMLLRNNEGEWNRWANANSGKY
DAAGKPVYDYTVNPKALEAYWRERVEKNKDFEGIYNIGMRGIHDSGMTFQGLSNPTLAQK
IAVLQEVIDCQRRILSEVLGKPAEEIPQAFIPYKEAAEYYNGNATTPGVQIPEDVILMWA
EDNHGYLRQTPTEAERQRSGGSGVYYHVSYWGVANTSYLWLNTTPLSQMYEELKKAYDTG
AQKYWILNVGDIKPSEISLEFFANLSRNIDFCDDTNIEEYIARTAQRDFLLDGAQAAEVA
DILTHYYQYNIAKRPEFQGYESSNGNDPYSVTANGDEAQIRVDQLRALADRAGAIYDAVD
PARRDAFYEMFYYPLRSSANSLAMHVYWQKNQLYKKQGRYASVRKYQDLSVAAYDGILSD
MIYYNKTIRDGKWDKIMNPYNGSIPVAVGLRSFATVEAADAKPGIGAVCEGQELPGDAVT
LTFSSLSDNRRFIDIYSREEAANDWTVEVSDDFIQPSETSGSVAAETRIWIGIDWSKAKR
GENTGTVTVRDRKGTEIIFQVSANKTVSRLSGKTYAEENGLVAIEAEHYSESVAKNGCAW
RVVKNLGRSGDSMKLYPDLAPRVDEDFENTAAQLKYNIYFESSGTFTGTLYRIPTLNEGS
ENGVAKTCRIAVGMEGKTPQVLSGNATCVDVNSRWKRNTLENIEKLTFTVQVDQPGVQTL
VVYQSDSGIAFDRVVINTGGEISSYLGPAESYNTIFFTKAPESRLPEEADNEPPRKIELY
QSADDAAPAIPVQDCLTMTPGDTVLLIGAVTGDLPADGIAWSVASGAEAVDLQPRADGSA
TLRASQAGEAILLAAAKGAGLTRYCKVIVSDTREVAGEFLEQNGEVMIDIVSALEQSPYA
NYSDSATHAWQLNADGNALTVQPDIGIKWQGDAKASTAVYDSAFLSAYAPQLNFQIYFEH
TGTYYIHTLSSHPIDASDSYHVGLDGVWQMHTNYATHQGKLRWEKRDSGWFITVDTPGVH
TLNIWPREDGIVSHRIYLTQTVGKSYFGGWGPAESARRTAAVGDKTALQALVTANASRKE
SGYTADSWAAFAKAYAAALERLGSRYTAQKELDAAAALLESAVSALQKNGWQEVAAQIDA
LPDRYTLTLSDAAAVAEARAAYDALDPSEQQRVENLPKLLALEKDLEIFASGQRGDVDQD
GKRTVSDVVLLRSVIVSGERLPAAQFWPADLDEDGKLTVTDVIALRQMIVKA

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	32	680	6.1e-202	0.8206599713055954

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	1.18e-143	186	557	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	2.80e-44	814	990	1	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam17829	GH115_C	1.55e-13	1119	1294	2	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
cd14256	Dockerin_I	1.14e-11	1434	1490	1	57	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUC17495.1	4.47e-277	33	1018	25	1040
APZ46852.1	4.47e-277	33	1018	25	1040
QEV23754.1	1.77e-240	16	996	13	958
AFO77172.1	1.08e-238	17	996	33	978
ADJ45460.1	1.08e-238	17	996	33	978

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZMH_A	1.25e-141	42	991	23	933	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
5BY3_A	1.85e-111	54	735	39	694	Anovel family GH115 4-O-Methyl-alpha-glucuronidase, BtGH115A, with specificity for decorated arabinogalactans [Bacteroides thetaiotaomicron VPI-5482]
4C90_A	5.74e-111	32	991	43	849	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
6NPS_A	2.41e-106	58	992	36	964	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]
7PUG_A	1.78e-105	32	993	16	836	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001806	0.952059	0.045300	0.000272	0.000277	0.000266

TMHMM  Annotations     

start	end
5	27