CAZyme Information: MGYG000000977_00085

Basic Information

• Species: Clostridium sp900539375
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium sp900539375
• CAZyme ID: MGYG000000977_00085
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
852	MGYG000000977_1|CGC2	98001.56	5.9423

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000977	2927434	MAG	Australia	Oceania

• Gene Location: Start: 79573; End: 82131 Strand: +

Full Sequence      

MSKEKKIKHEKKRKKRHIVRNIFLVLFLIIIISAIAAAFLGYNFYKKHKIEISNCIKDGY
KKVESVDENTFNGRHSTKIYDKDGNLLKEFKVNNSIYKSYNEINPLVFKASIAIEDERFY
DHKGIDYKGILRAGVKYLTSKGQEVQGGSTITQQLCKYTFLTLDKTMWRKLEEMVIAQEL
EKKYTKNEILEFYVNNNYFGYGCYGIESASEYFFQKSTNDLTLSEIAFLVGIPNNPTVYD
PINKIDNTLERRNLILNKMLELGYITKEEYDKAVKEEIVLNVNKVYFDNTVDDYALDLAV
NESTKILMKMKGFQFKYIFSTEEERVNYFDKYNELYNSCRDEFLTGGYEVNTTIDMELQN
QLQWYIDNELQGETEVDEETGIYKRQAAATVIDNSNGTVVAIVGGRTQEGNTYNRALLSA
RQPGSAIKPLIAYTPAFENGYYPDEMYMDEPIENGPSNWFDGYYGNISLRYAIETSVNTI
PYRLMESLGVDKCKKYLTNMEFKYLTPEDNSPILAVGGWSKGATTIEMAGGFSTLARNGT
FIEPTNVLKITKVGVDKEIYENAFLQKKIYDEGACYLTTDALKGVLTKEYATAHGSGIDN
FVNQAGKTGSTDEYKDLWMAGYTPYYTAVVWNGTDTPEPLYSSMSASKNIWKNMMTYLHK
DLEDKEFQKPESIYEENGQLKNNLSSGENKILLARKNEETTRKSSEINEQTERLKENSYR
IEYGLSDKEEEAREKKAKIALEELSEYNLTKSTQEEELNKLLEKCSRKIEEVKKVSSYNE
YILEYKNLKTYFTNKLNSIKAEEKRKLEEKNRINKPSTSTAITTSTANKTNNKTNNKTTT
TTATTKNNNKNN

Enzyme Prediction     

No EC number prediction in MGYG000000977_00085.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	83	259	1.1e-64	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	5.26e-164	61	687	40	623	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	6.68e-161	97	661	4	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	3.34e-133	77	717	49	781	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	1.81e-79	70	673	108	695	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam00912	Transgly	2.76e-76	83	260	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFV21847.1	7.26e-250	36	810	43	808
AMN31373.1	5.16e-222	5	809	8	814
APC42268.1	4.00e-211	24	684	26	681
AJD93619.1	1.07e-182	16	809	32	823
QZO10429.1	4.68e-171	44	697	53	710

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7U4H_A	1.12e-65	76	670	20	772	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3ZG8_B	1.21e-61	178	650	1	449	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3DWK_A	6.42e-60	78	673	9	602	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
4OON_A	9.64e-60	78	661	22	725	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	1.04e-59	79	655	23	712	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P40750	7.16e-85	79	655	58	620	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2
P38050	4.04e-84	66	656	38	595	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q04707	3.03e-80	62	674	38	641	Penicillin-binding protein 1A OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=ponA PE=1 SV=2
Q8DR59	5.65e-79	62	674	38	641	Penicillin-binding protein 1A OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=pbpA PE=1 SV=1
A5I6G4	1.53e-76	79	673	59	658	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.996267	0.001102	0.000033	0.000012	0.000005	0.002607

TMHMM  Annotations     

start	end
21	43