CAZyme Information: MGYG000000990_02055

Basic Information

• Species: Blautia sp900539145
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp900539145
• CAZyme ID: MGYG000000990_02055
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2088	MGYG000000990_44|CGC1	231557.08	4.3464

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000990	2534213	MAG	Denmark	Europe

• Gene Location: Start: 14252; End: 20518 Strand: -

Full Sequence      

MKKKEIFKKTMATMLSGVCIVSGITMPTETVHAGLGYWPEDLPVPNQFYYQNESLQPYGT
CLQVGELKNWSPDNDPDARYNRSAIPLRERYMGPNVNPLASRDAKVQPLAMSNARASEAP
SQGGDGDFVYAFNNFQYVDTYNFWGGSSAEGPIAIPSPEHIDSAHRNGVMATGTIFIPWG
DYAYGNQFVQELVEKDEEGNFVCADKLIEIAQYYGFDGYIFNAESGTGVQGFKEFLIYMQ
EKAPENFRVSWYNGRGSLGDTDIKNWMQDGDKRVNDEWWLDMSGSGYVDSTIDAAYAADR
DKWDIHSTWEYIPMEPGAKGGNYQTRLDKDGKLKISLGILAPTSTLTQSKSSDDFMNVQD
QELWVGPDFDPSSTNRPQNEFCGFANLVADQTPIIGTDFVTHFNPGNGYKFYENGKVTGK
KDGWYNRSLTEVLPTWRWIVEAEDGGSEVTPKIDYDDAYWGGASMKISGDLTAEKANHIK
LYSTQLDIQDNSTFELVYKTKKDTDVKLSVGLCFGDTYDEENFEFYPVEVKGDGNWQETS
IPLANKAGEKAIAISLKLESAKGVKDYDLSIGKLGVVTDKSKPAAPGEITLDDVIYPTDV
TAEARIYWEKGENSFIDVIHRVKPDGTKEFVGATPSDAFYISKYKKLEGEKEATFEITSF
SENGVAGASKQIKIQWPEEVPGGYGPELELGKNLALRAPAVTDTPCAADGPVDKINDGVI
PSSKWCGLTTNGSAYLDLGKEVDISRWIVYHANCRGAGEGVDYNTIDFELLYAGDDGAEL
LDPDDSSAARRVKSLSYKEADRVRGNKQDVTDRNLNDPIKARYIKLHVTTSDSTPWSAIR
IYELQLYENAGEGSVTVSSSPLERNVTVYNNEGNKDKVIIDNVTMLYSKGNYPNGTVNEK
TGVVYLYDSMEAKEPIAQVRAEQPDNVYKQYSKGIATFENLELNPNGGRLWYSVQDASTS
EIVQSQRTSVAYPPETGEEIAQGEAKLFARAAGVNLTNPYGALEIKGLEENAVVKVYADE
NAAKPILVSAPAGKDQTVVLEQVPLTADGGKVFYTIHAPKKPDTERLAKEYGKVSDMPAY
QKDLKEMVDMYESKFEASDYIDSTWGAFETARSNAAALVGAEATKAQADAARMALKDAEC
ELLKDADKSRILEVCEEYEANRDHYSKDSWEAFKPVLDSVSAFAREEAETDKMTIQTQRV
KLDKAARGLVEIVPVDSTVIPDEALLKAIKEQTGCNSAEEMAQYEGELDLTGLPVKDLTG
LETLKNLKGLTLKGTQVKEVLGRNIPKTVEKLDMSDCAELKTIDLRDGGYTNLKEANISG
CEKLVLVYMNSVGLEKLDVSENGKYEKVYAVDLSGNKLDLSKETPEGKFVNKVEENLKAN
PPKALYNYNNVSKGMRIEADVMAGRHEEPEVMVDGDKTQLSGGYYLDNKGTATINLGETR
EISKVAVTYREKEVASEYKWEYSEDGRNFKEIVTVPDNKKASAEYVLEKPVKAHYIRYTT
VNKGNGEAVILELEAFTREKVESNVKYTGQAPLLTVDYESIPAEIQITEGSEVVKIQDYM
DKCYKESKTVRGTVATALKGQKWLAEDYDIDAMTKVPEGMKVVITGADGVVYEDPFNPEK
VGTYTVEFKSQDDTVLATMTIKVSADKEALKAAIDAAKKLEEKNYTPGTWAPFAEALKAA
EEAYANEEATTEMVQNVVSALADATEKLVEKPSKDALKAAIDAAKELKEENYTPETWAPF
TEALKAAEEVLANEEATAEMVQKAKDALVDATGKLVEVAPEPEKPSKDALKVAIDAANAL
TKEDYTEETWAPFAEALKAAEKVYADEEATVEDVEKAVTDLQEAQNNLVEVIKGWHETEN
GLEYYENGKKVIGWKAISEEWYYFNENGIMQTGWVSVGGHWYYLNEDGTMETGWVSVGGH
WYYLNEDGTMETGWVSVGGHWYYLNTDGTMETGWAFVGGHWYYLNEDGTMATGWVSVGGH
WYYLNTDGAMKTGWVLVEGHWYYLNEDGVMETGWLQVGNTWYHLDNSGVMETGWVSVGGH
WYYLNTDGSMETGWVLVGNDWYYMNTDGSMATSQWIGGYYVDASGKME

Enzyme Prediction     

No EC number prediction in MGYG000000990_02055.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	111	412	3.8e-53	0.9968253968253968

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	5.08e-122	66	576	38	549	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
NF033930	pneumo_PspA	5.12e-80	1715	2085	298	657	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033838	PspC_subgroup_1	4.67e-71	1711	2086	301	681	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.
NF033838	PspC_subgroup_1	3.30e-66	1909	2087	483	665	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.
pfam03644	Glyco_hydro_85	3.79e-65	123	408	1	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASM70437.1	0.0	3	1648	2	1649
QQA11602.1	8.97e-192	45	1061	36	1019
AMN35046.1	8.97e-192	45	1061	36	1019
ABG82927.1	1.24e-191	45	1061	36	1019
AQW26114.1	1.73e-191	45	1061	36	1019

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3GDB_A	2.80e-102	66	841	170	937	Crystalstructure of Spr0440 glycoside hydrolase domain, Endo-D from Streptococcus pneumoniae R6 [Streptococcus pneumoniae R6]
2W91_A	2.05e-85	66	676	19	634	Structureof a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D. [Streptococcus pneumoniae TIGR4],2W92_A Structure of a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D, in complex with NAG-thiazoline. [Streptococcus pneumoniae TIGR4]
3FHQ_A	5.75e-85	66	675	15	617	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHA_A	7.79e-85	66	675	15	617	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	1.00e-83	66	675	20	622	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A1L251	4.52e-24	29	512	35	489	Cytosolic endo-beta-N-acetylglucosaminidase OS=Danio rerio OX=7955 GN=engase PE=2 SV=1
Q8NFI3	9.90e-20	35	516	63	517	Cytosolic endo-beta-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=ENGASE PE=1 SV=1
Q8BX80	4.52e-18	35	516	55	509	Cytosolic endo-beta-N-acetylglucosaminidase OS=Mus musculus OX=10090 GN=Engase PE=1 SV=1
E8MGH9	1.04e-15	1646	1833	1667	1860	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1
P0C7A1	5.05e-12	28	471	56	463	Cytosolic endo-beta-N-acetylglucosaminidase OS=Gallus gallus OX=9031 GN=ENGASE PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000643	0.997859	0.000821	0.000291	0.000192	0.000163

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000990_02055.