CAZyme Information: MGYG000000990_02156

Basic Information

• Species: Blautia sp900539145
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp900539145
• CAZyme ID: MGYG000000990_02156
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
634	MGYG000000990_50|CGC1	73734.54	6.7645

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000990	2534213	MAG	Denmark	Europe

• Gene Location: Start: 7978; End: 9882 Strand: -

Full Sequence      

MGVNMKITELDRYLFGEGTHYEIYKLLGAHVGVNRKKEGVFFAVWAPHAKSVSVVGNFNN
WDAKADIMKCDNNMGLYQLFIPKVREGDLYKFCITAQSGELLYKADPYANYSELRPGNAS
RVVDLTGYKWSDSVWMKKRQEFDVDKDAMSIYEVHPGSWKKHPAQGEDDPGFYNYRELAH
ELAKYVKEMGYTHVELMGIAEHPFDGSWGYQVTGYYAPTSRYGTPEDFRYFVNYLHKNKI
GVILDWVPAHFPKDAHGLADFDGAPTYEYADPRKGEHPDWGTKVFDYGKNEVKNFLIANA
LFWIEEFHIDGLRVDAVASMLYLDYGRRDGEWVPNQYGENKNLEAIEFFRHLNSVVLGKH
KGVVMIAEESTAWPMVTGKPEDGGLGFSLKWNMGWMHDFLEYMKLDPYFRKYNHHKMTFS
TSYAYSERYVLVLSHDEVVHLKCSMINKMPGLGQDKFENLKAGYSYMIGHPGKKLLFMGQ
DFGQYQEWSEERELDWYLLREENHQQLQNYVKELLKMYKKYPALYANDSNYQGLEWINAD
DATRSIYSFVRWSPTGRNNLLFVINYTPVAREDYRVGVPKRKQYKLILNSKEPEFGGDVH
KEVVDYKAVKKECDGRPYSFAYSLPAYGVAVFRF

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	185	484	1.2e-154	0.9933554817275747
CBM48	27	111	4.5e-17	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	8	634	8	627	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	26	634	26	621	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	5	633	4	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	0.0	7	632	603	1220	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	7	633	100	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASM70613.1	0.0	1	634	1	634
AWY99231.1	0.0	1	634	1	634
QIB55783.1	0.0	2	634	4	636
QMW76350.1	0.0	2	634	4	636
ASU30768.1	0.0	5	634	7	636

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR2_A	2.34e-234	7	633	129	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQW_A	2.34e-234	7	633	129	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	3.31e-234	7	633	129	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	4.69e-234	7	633	129	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	6.65e-234	7	633	129	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	4.81e-297	1	634	1	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q8DLB8	6.60e-240	6	633	105	749	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
A1U0K1	6.89e-239	7	633	6	628	1,4-alpha-glucan branching enzyme GlgB OS=Marinobacter nauticus (strain ATCC 700491 / DSM 11845 / VT8) OX=351348 GN=glgB PE=3 SV=1
Q7NL20	2.64e-236	7	633	103	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
B3PGN4	9.86e-235	6	597	114	699	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000990_02156.