CAZyme Information: MGYG000000990_02240

Basic Information

• Species: Blautia sp900539145
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp900539145
• CAZyme ID: MGYG000000990_02240
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1807	MGYG000000990_56|CGC1	199783.4	4.6267

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000990	2534213	MAG	Denmark	Europe

• Gene Location: Start: 5481; End: 10904 Strand: -

Full Sequence      

MRKQRLARMGAATLAAVLTVQGMGFSSAVYAKGEMVRVKEDSQSQMSSEPERVTVKDYGS
SSVRTQNFDSDWKFNLGDVSNAQNPTFDDSKWRTLSLPHDYSIEQEYSQSLEAESGYLPG
GIGWYRKNFTLGEEAKGKRIRIDFDGVYMNATVYVNGKEVGTHPYGYTPFSFDITDYISY
DKENTIAVKVDHQTPSSRWYSGSGIYRSVSLTTTNAVHVDLNGIKVESKNLATEAGKTVN
TDIKTTVVNDSKEAQNVTLTHTVFKKGEKPDAAIGTFTTEAQQVGAGEKTEISATVPVKN
PELWSVENPALYTIRTEVKAGDKLLDSYDTEYGFRYLNFDTETGFQLNGKNVKLKGVCMH
HDQGALGAVANRRAIERQVEILQEMGCNSIRVTHNPASKDLIEICNEKGILVIEEVFDGW
HRAKNGNSNDYSVWFEKTIEENNTILGKEDGMTWAEYDLKAIMKRDLNAPSVIEWSLGNE
IQEGAGGSGYAERADRLIAWAKEVDETKTLTIGSNAVKRGDWEQVSIGDKLTKAGGTSGT
NYSNGASYDQIHREHPDWKLYGSETASSVNSRGIYSVTGSQQATSEQQLTSYDNSRVNWG
ALASEAWYDVIQRDFVAGEYVWTGFDYIGEPTPWNGTDPGPKGTWPSPKNSYFGIIDTAG
FPKDSYYFYQSQWNDEVNTLHVLPAWNEDVVKKNADGTVPVVVYSDAKEVELFFTPADGG
EKKSLGKKTFKTETTKAGYSYQVLENGKKNHKDLYMEWQVPYEAGTLEAVAKDAKGNVIK
NAEGRSVVKTTGEEAKLSAKADRKSIQADGKDLSYVTVDVTDKDGNIVPDAANRVTFDVQ
GAGKLVGVDNGSSPDHDSYKADNRKAFSGKVLAIVQSTEKAGEITVTAKADGLEASTVKI
TTTPVKEEAPERYIESYKYSKSYYVKTGTKPQLPEKIEAQYSDGTKEDVAVTWGEITDEQ
VSKTGSFSVEGTVGKKKITVHVNMIDDVAALLNYSGVTQKGVKPQLPDVRPAVLPDGTVL
SASFPVQWEEKNANAFQNADEIVTVNGSADIFGKTIPVTASIRVQKEDIKIGSSVTNVAK
LSQNIQGSDTLEAIKDGKTAMSLNNDGGPNESAWSNWDAAQKGTKEAELTFTFDTQQRIG
EIVIHFAKDNNSIRFPDEGTTEIFVSETGKDGSWEKVEVKEHIGQEKDRVKAYRYEMAPV
TATYVKVKVVNANATDTGNRKPCTAITEVELKKAEGTFKVNETAELAEVKVGERVLPKAA
YVLDSYSVPETNVKVTAKAKNNASLTILPKHENVVRMILESENHKTTKNFAVRMGEEETA
LPEDDSRDYPVEKITATAGSAYTPGTANEGPVKYVLDGKANTHWHTNWSVAGEGSKPEHR
TVTLKLGDDEEEAPKIDALRYMPRMQGGANGRMTEYEIQYSLDGKEWQTAATGNVDKQQG
GWIILGFEEPVQAKYVRLIGVHTASDQGNDKHMAVAELRARVAIEVPTPPEKFTITASVN
DETMGTVTLDSETGVYEKGANATLTAKAKEGYTFVNWTIDGEEVSTENPYIHTVEADTTI
TANFKTIEVENEGWVQTENGWEYYENGQKAVGWKEISEKWYYFSETGIMQTGWVSVGETW
YYMNESGAMETGWVSVGETWYYMNESGAMETGWVSVGETWYYMNESGAMETGWVLVGDTW
YYMNTDGAMETGWVLVGDTWYYMNTDGAMETGWVSVGGHWYYLNADGAMETGWVSVGGHW
YYLNADGTMETGWVSVGEHWYYLNTDGTMATGWVFVDGYWYYLNTDGTMASNQWIDGYYV
DASGKMA

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	54	815	1.4e-122	0.7792553191489362
CBM32	1337	1478	4.5e-19	0.9596774193548387

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF033930	pneumo_PspA	9.10e-85	1592	1804	444	657	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033930	pneumo_PspA	1.98e-78	1610	1807	442	643	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
COG3250	LacZ	6.03e-72	60	926	9	791	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
NF033930	pneumo_PspA	4.77e-71	1628	1806	440	621	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033838	PspC_subgroup_1	7.90e-70	1610	1805	485	681	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASM70369.1	0.0	1	1605	22	1625
QRT48501.1	0.0	3	1503	4	1502
ALE10930.1	0.0	39	1495	38	1501
AFL04054.1	0.0	39	1495	38	1501
QRI57590.1	0.0	39	1495	38	1501

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QUB_B	0.0	64	905	8	856	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]
5DMY_A	0.0	39	924	12	904	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]
6QUB_A	0.0	39	911	6	885	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]
7NIT_A	0.0	39	1328	6	1304	ChainA, Beta-galactosidase [Bifidobacterium bifidum],7NIT_B Chain B, Beta-galactosidase [Bifidobacterium bifidum],7NIT_C Chain C, Beta-galactosidase [Bifidobacterium bifidum],7NIT_D Chain D, Beta-galactosidase [Bifidobacterium bifidum],7NIT_E Chain E, Beta-galactosidase [Bifidobacterium bifidum],7NIT_F Chain F, Beta-galactosidase [Bifidobacterium bifidum]
4YPJ_A	1.96e-224	64	904	8	810	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.22e-171	68	901	47	847	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.74e-110	67	890	50	808	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	1.63e-59	67	905	7	741	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	3.28e-54	60	905	23	798	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
T2KPJ7	1.86e-49	66	723	54	676	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000338	0.998795	0.000322	0.000211	0.000167	0.000142

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000990_02240.