CAZyme Information: MGYG000001004_01787

Basic Information

• Species: CAG-56 sp900539525
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56; CAG-56 sp900539525
• CAZyme ID: MGYG000001004_01787
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2002		216182.43	4.7268

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001004	2903340	MAG	Austria	Europe

• Gene Location: Start: 2511; End: 8519 Strand: +

Full Sequence      

MRKRILSALLSLSMVMSMSGMTVMASGSRTARVEETTSTVEAGNSLRLWYDKPASQGGAG
NVTDANEVWQQFTLPIGNGDLGANIYGEIVNEHLTFNEKTLWTGGPSDSRQDYMGGNLES
SGRNGATIREIQNLFATGQDAAASAKCGDLVGISNGYGAYQAWGDIYFDYTGYAEGDAQG
FVRDLDLTTAISTVRYSAGGKEYSREYFVSNPDHVLAAKLEASGNENLSLDVRFTSKQGA
TAVASGDTLTVKGQVSDNQLKYDSILKVVNDGGTVTANGSKLEVRGAKSITVFLTAATDY
KNDYPEYRTGETADQLHTRVEKVLNDAVAKGYERVKEDHIKDYSELFSRVDLDLGQTVSS
KPTNELLAAYKAGTASEAERRQLEVMLFQYGRYLTLGSSRENSQLPSNLQGVWNNRNNPP
WASDYHMNVNLQMNYWPTYSTNLAECAKPLISYVDSLREPGRVTAKIYAGIESTTENPEN
GFMAHTQNTPFGWTCPGWSFDWGWSPAAVPWILQNCWEYYDFTRDTDYLEKNIYPMMKEE
AVLYDQMLIEDADGDLVSSPTYSPEHGPRTNGNTYEQSLIWQLYEDTITAAKTLGKDSDK
VAQWTANQAQLKGPIEIGTDGQVKEWYSETSLGSAGGEGFGHRHLSHMLGLYPGDLISVE
TPEWLEAAKISMNNRTDSSTGWGMGQRINTWARLGDGDRAYKLITDLFKGGIYQNLWDTH
PPFQIDGNFGMTSGVAEMLLQSNVGYINLLPALPSVWEDGKVDGLMARGNFEVDMDWFDG
HLDHAVITSNNGGEAVVQYDNIFLSTVTDEAGNDVEVTKISDDRISFPTVKGKSYQISQV
PVKETVETPSGLSAARISSNQVSLAWDAVEAENATYNVYRKIGEGELVQIATEVSENKYD
DTKAYELMGEVSYRITAVVNGTESEPCLAVKALDYRNMAGMIDDQDPRVVYSGNWGNWNR
ETDGNYNNTIKYIQDNLTGNDTATLTFSGTGIEVYSCTNSDRGYVEISIDGKVEGKVSTY
SSSTVRQAKIFSKEDLEPGLHTIVVRVTNDRETSSSRNKVEIDAFKVLNTQEVAVTNVNV
ETKSGIHTVSKANSTLQMIAEVQPADATNRNVTWSVATKSGGAAGQIDKNGLLTLNGESG
TVTVTAAAVNSTVTGTLDINVAIAGSTEISTVVEDSVNNSSPNPAITWNGSWSTWAGEST
RHHGGTKTEVRTEGSSFSYTFTGTGVSVYVQKHANFASLKVTIDDEDMGTYSMEGSSSGD
DQQLLYSKKDLADQQHTITCTVVARNGKTQANLDYIETFTQTSNSDKAALQTAIETHENK
KEAFYSEGTWAAFKSAMDSAVAAMNDPEVTDVTSYVTALNNAAANLVEKDAAAPVITNES
GSAVAVETKRVMLSWDPVDNADFYNVYIGADKVATTAQTGTWINDLSAGTEYTFTVKAVN
TVGESTGSITVNTTTLAETTSENTEAPATVTNVNMTKNDSDKTKATLSWTAADKAVKYAV
YVNGNKAGETTGTEYALTNLEGGYKYNIKIVSLDANANSSAAKAYSFVIQAEIASVSALE
GVTVPLGTTFEQLKSVLPEKVSVTLVDSTATPELEITWNPGSYKADTAGTYTLSGDLNLT
EAVTNAGQKKASVTVTVQEPRDISSVAALDPVTAGVGTAFNQLTLPETVTVTLKNNETLS
LAVTWLAGNYNGNAAGEYTLKGNLTLPEDITNTDNLQAEIKVIVEAAAPSDVKILSVASL
PALTVKKGTTFEQLALPKTVAVKVENSDADVTLNVTWQKGNYNENAAGQYTLSGELQMAE
GIVNPDKLQAVITVHVQEDTSKPDPKPGVPDPKPGVTEGQVLDSGNYSYKVTSTSNLTVE
VVALKNKNLTTAKIYNTVTLGGKSYRITSVAPSVFKNNKKVKSLTIGKNVEAIGSSAFAG
CTNLKKVTVSSTRLKTIGAKAFSGCKNLTSIKIKSKVLKKVGKNAFKGINKKAVIKVPAS
KLKAYTKVLAKKGQSKTVKIKK

Enzyme Prediction     

EC	3.2.1.63

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	43	801	1.3e-254	0.9847645429362881

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	1.11e-64	48	302	1	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
sd00036	LRR_3	2.69e-20	1867	1980	14	116	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
sd00036	LRR_3	2.04e-19	1887	1980	2	93	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
sd00036	LRR_3	2.65e-18	1865	1968	35	127	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
pfam13306	LRR_5	4.70e-18	1863	1970	7	102	Leucine rich repeats (6 copies). This family includes a number of leucine rich repeats. This family contains a large number of BSPA-like surface antigens from Trichomonas vaginalis.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHB24557.1	0.0	64	1547	54	1562
QEI32060.1	0.0	64	1547	54	1562
QRT30709.1	0.0	64	1547	54	1562
ASM69030.1	0.0	35	1552	30	1562
QNM13266.1	0.0	46	1369	44	1364

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2EAB_A	6.09e-237	48	798	20	850	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	4.58e-236	48	798	20	850	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]
2EAE_A	8.68e-236	48	798	19	849	ChainA, Alpha-fucosidase [Bifidobacterium bifidum]
2RDY_A	1.68e-132	45	839	2	791	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	1.02e-119	38	792	14	736	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	1.03e-109	46	782	52	797	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	3.69e-88	48	779	23	758	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	2.81e-85	43	777	19	775	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	2.89e-69	58	804	8	722	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2
P20533	8.51e-08	1370	1542	461	636	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000455	0.998585	0.000276	0.000294	0.000195	0.000159

TMHMM  Annotations     

start	end
5	27