CAZyme Information: MGYG000001018_01270

Basic Information

• Species: Mediterraneibacter sp900751785
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900751785
• CAZyme ID: MGYG000001018_01270
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2401		264931.19	3.9047

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001018	2841260	MAG	Sweden	Europe

• Gene Location: Start: 55; End: 7260 Strand: -

Full Sequence      

MKKMQKRAAGFILAICMILSCIPVPAAAAASDNLALNKPASASDVESGTSFTADKAVDGN
SGTRWATNRDTNAVKTARWLQIDFESTVTFDTVDISWEQANILSYEIQVSDDAGEDSWTT
VYSRESEPTMTDESITLSAPTSGRYMRIYVSDFGGDWASVSIYEVGVYYLAGGGDPSDSD
GNYTIYPIPQKVTDEDGTVELTDTVNVVVEDGIDAVTHDRVDEVLTENGLTPQYSEDPSD
DYTNLYVGINGSGGAADSLEDIPREVFDEGENKYDMHVVKVFEEGDIVVLGKDTDAAYYG
LATLEQMLDQTEDGQLKISTFEDYAFQKYRGCVEGYYGYPWSVEGTLSWFDFAKKYKMNV
FLYGPKNDPYHLGQWDEDYPTEVTEEESKLGVRTQDEMRQIAEKAAQCNVDFVWVAHPAM
KKPIDFTNEETVDEGVGRLMVKFDHMYDLGVRQFGIFVDDIDMSTAASTCDMQIYMLNQV
QEKLYAAYNTEGAAAEDCVKPLFFTPAWYTTGSGGASTYMPKFRALHEDIEICFTGNNVF
SDISNSSATTFKNWIGRDPVMWWNYPVNDNVDNVYYTNPIDHFYSLDSNPSNLKGVISNP
MNYSESSKVAFFGLADYTWNPQAFDAEENWEHCFDAILPDDPEMAQALKVVYGSLNYDYE
PSELTRLYAQYGSGNEEAAPALMEKMYEILDSVEKLETLKDSEDPVKRLIVEEAQASINK
LYDMAAAIGGAMAAVSSDDPLVQTHGYYIANEAYERFSIDRNPRYQIIALEGSGEDYYYS
IWYANPSDNRMKGFVTTAMEAIEDFDIGALDTSAAEINSVEISPSESVVVRQGESLQFTA
NVDAAQEDTGDVIWTVEGGSGAGTSINWNGILTLDTNELSPTVTVKAVSAYDDTVYDTVE
VTVADRIYVDPTIPVNQAFGAKVLGGSGSPGVGGGPENLFDESEDGSKWCPGDNTRFNQW
AAFDLGAEKTISTWETVHAGVEHELDISSDFSLQVLADPNATEEQLSDRSYLANNSNWTT
VAEYRDNTENITDYEFEEPVTARYFRLYISDGCQDNVPYPATRIYELRLFGVDTATVART
HSLTIDPSIIGGTITTDAAHYEEGAKVNIYIEPDEGYKLAEGSLKYNDTVVEGTSFLMPA
EDVVITAEFTEGSEEPDPGWEDEDALAALVNPVVQKYELAENSGTWTMTADTRFAVTADQ
ENIENERLAEVVELINSEITEKEIVSSSPLAMVYANEEDAQPSDILITLDKDGAVCEESD
SEEAYTIVIGKDGVKINGASENAVLYALRTVQNYMVANKGLPYGTITDYPDVAERRLHVD
CGRKYFTKDWFIRQIREMSYMKMNTLEMHFSENLGFGIECKTDPAIVSDQYLTHEEVREI
LAEAKKYGIKVIPSFDSPGHVDQILKAHPEYGQVNINGDHYASGLDVTNPEAVAYIRTLY
DEYMELFEGCTDFHIGGDEYMEFDRAPFTTQYKPVLNEYAAETLGEGATWKDVMANYINE
LAEYVYSKGFKPRVFNDGLYYGETDWEGPQQIVMHDYIGIDYWSKMTWNPAIAGMQTFID
KGHEDIYNFNSTFFYYVLRNDMPTDGREQHSFDYIDQDRRIFEEWTPGKFASYTAPDDAS
YIAGAAMGIWCDNPDLVTEDVVTDDIADEMRSLATKSWNVSSSDNMDFEQFKANYEVLGH
VAGYEKGSQLPDAGEFQNAESLGKVTLCYVSDTGKTLRDNVVKYGNVGDTYSFEAEDIYG
YRLISEGTVSGVYSSEGETYTFTYTLDTDKSELEQELEDALDAAYYIPETFEAYSQAYAA
AEEAVERADIEQAEIDEILAQLREAKGQAVLLVNYPLYVETKYPLSDIGYAAGYEEYEAA
VEEGREILYAQGDDPAAVKAAFDKICEAAEGLQKPGAGAPEISADDGYYTTYSYDKMLDG
NESTKCWFNKDQTAGRQFMFTFPVTVSLSGIRILQPADVGADYIEGAEVQISADGESWQT
VGTIGPDDRDTELSFEAVPAKYVRVVLTETKKNWYQIAEVSFTYEEIAEDTTLRDLIREA
EGLDISEKDTELVNAMISALIEAQKLYAQGSTDTDAVQAAAAALEAAIEALKENPAPGGE
ISTAILEYAIELAEGIDIDSSGVIDAVKENFETALQNAKDILARVQAGDTDVTESDVDSA
WRNLIQAMQYLEFKQADKTDLEKVIALAEEMNKNLDAYLDAGKEAFTSALAAAKELYADG
NVMDQEEVNSAWQNLLDAMANLMLKPDKGLLEDLIAQAEGLSEADYEAQSFAAMRAALAS
AKDVFANEDATQEEIDASAAALKDAVAKLTLADSGDGQQGGNTGSGQSGNGSQDQGQTGG
SSDKSNTSSTAGKTSSAQKSSGTQQSAPTGDEGNYIFWGAAAVLAAGAAAAAAYRRRQKR
N

Enzyme Prediction     

No EC number prediction in MGYG000001018_01270.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	330	650	6.6e-74	0.9864406779661017
GH20	1314	1659	8e-61	0.9495548961424333
CBM32	42	165	1.6e-22	0.9274193548387096
CBM32	933	1067	2.1e-16	0.8548387096774194

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	2.69e-102	330	650	1	292	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
cd06564	GH20_DspB_LnbB-like	1.01e-61	1314	1658	2	324	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
cd02742	GH20_hexosaminidase	4.43e-34	1315	1658	2	302	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	1.15e-32	1315	1654	4	337	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
COG3525	Chb	7.70e-29	1262	1633	205	590	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCL57158.1	0.0	1166	2319	55	1204
ASK64148.1	1.45e-228	1164	1701	32	577
APC47599.1	7.44e-221	1164	1705	32	581
AYV34923.1	5.33e-173	1170	1698	41	569
BCL57159.1	1.86e-162	1169	2242	45	1076

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	4.67e-77	169	643	20	468	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV4_A	3.62e-71	183	634	32	473	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PV5_A	3.71e-54	177	650	34	485	Structureof CpGH84B [Clostridium perfringens ATCC 13124]
2J4G_A	1.40e-51	284	643	86	415	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2J47_A	1.42e-51	284	643	87	416	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with a imidazole-pugnac hybrid inhibitor [Bacteroides thetaiotaomicron VPI-5482],2W4X_A BtGH84 in complex with STZ [Bacteroides thetaiotaomicron VPI-5482],2W66_A BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W66_B BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W67_A BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2W67_B BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2WCA_A BtGH84 in complex with n-butyl pugnac [Bacteroides thetaiotaomicron VPI-5482],2XJ7_A BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XJ7_B BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XM1_A BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM1_B BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM2_A BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],2XM2_B BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],4UR9_A Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],4UR9_B Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],5FKY_A Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FKY_B Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FL0_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL0_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	2.25e-67	183	634	39	480	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	1.03e-50	284	643	108	437	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	9.14e-47	187	715	48	531	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	4.86e-46	187	715	48	531	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
B2UPR7	1.84e-23	1243	1681	167	590	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001043	0.683184	0.314634	0.000552	0.000308	0.000250

TMHMM  Annotations     

start	end
7	29
2375	2394