CAZyme Information: MGYG000001024_02161

Basic Information

• Species: HGM11521 sp900751885
• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UBA1381; HGM11521; HGM11521 sp900751885
• CAZyme ID: MGYG000001024_02161
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
906		101664.67	4.8364

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001024	2614111	MAG	Sweden	Europe

• Gene Location: Start: 216964; End: 219684 Strand: -

Full Sequence      

MKTWNMDRGWLFHLGDIPKPEETGHTFCYMAAKAGGAVGAASPDYDFSGWQEVDLPHDWA
VYGEFGEQYGPSAGYKPRGKGWYVKRFKLDKEAEGKQILIEFGGISSHAVVYCNGSVVGR
NFCGYTSFTVDITDMALYGDRVNTLSVFVDATEIEGWWYEGAGIYRHVTMYEKEPVHIAH
WGTWVKPQKQDNGGWNTQIETTVENSSYETAGFEAVAWVKDAQGTVCGQGHTEGSVSAGD
TQKVSVNIPIDAPRLWDIDDPYLYTLVNEVYIEGTLCDRQEVPFGYRSISISCDDGFFLN
GRRVQLYGTCNHQDHAGVGIAVPDAVVEYRIRLLKEMGSNAYRCAHGNPSREVLDACDRY
GLLVMDENRNFSSSPEGVEQVRQMVLRDRNHPSVVMYSIFNEEPLQGTPTGRRLAERLAC
EIKKLDDTRFLTGAMNTGVSEEDSAARALDIVGFNYITHLFDEFRERFPDKPMLGSENDS
AFATRGVYETDENAHVIDNYDSVAAPWGNTYRDGMYQIESRSHIMGMFVWTGFDYRGEPT
PFEWPSIGTQFGIMDTCGFKKDVYYLNQAYFKEEPVLHIFPHWNWDEGKQVRVMAYTNCE
EVELFLNGTSLGRKKTPRYEMADWMVSYRPGVLCAVGFRGGQEAAKDSIKTTREAKRLRI
EASRELLWDGCDDTVVVNVSVQDADGENLPTDNRLVHFQTEGGEIIGVGNGDPNSHEADK
ADKRRLFHGHCQVIVKQTDGAKTVTIRAEADGVEGARIALPVQKAEHAVPYLPSVMESYL
STWRKNVTPFSEKPNPMQKIEDHDMNTWAVVRAGEQEKPDAGYLLYKTTLNLPEDGGKTI
SFAELTAQQAEIFLSGEKVFEGGCEYGRSVSIQTDKVGEQTLSVILYNQTADKRTGLTKS
VILRKD

Enzyme Prediction     

No EC number prediction in MGYG000001024_02161.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	548	2e-104	0.586436170212766

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.61e-50	43	543	44	565	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	9.68e-41	46	535	67	548	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	2.97e-40	79	432	65	446	beta-D-glucuronidase; Provisional
PRK09525	lacZ	1.35e-29	81	402	122	462	beta-galactosidase.
pfam18565	Glyco_hydro2_C5	5.16e-29	658	758	1	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANE47110.1	1.29e-316	5	905	21	941
AZK45303.1	1.71e-298	5	904	6	917
BBF43905.1	1.33e-268	6	892	13	906
BBB68905.1	2.62e-247	5	902	39	950
BBB62958.1	2.62e-247	5	902	39	950

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	1.24e-141	40	760	23	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.76e-139	35	753	24	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	7.70e-137	35	763	39	821	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CU6_A	3.36e-113	40	766	33	848	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	9.13e-113	40	766	33	848	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.11e-151	10	764	51	850	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.03e-82	42	746	62	804	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KN75	1.85e-60	44	781	49	817	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	2.58e-52	44	707	26	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	4.75e-51	50	718	79	786	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001024_02161.