CAZyme Information: MGYG000001050_00316

Basic Information

• Species: UBA11490 sp003524095
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; UBA1242; UBA11490; UBA11490 sp003524095
• CAZyme ID: MGYG000001050_00316
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
808		92331.11	7.8879

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001050	1065888	MAG	Denmark	Europe

• Gene Location: Start: 11066; End: 13492 Strand: +

Full Sequence      

MGIKMTEQKALTLLQENLSKYSELTLKEATAKDLYKVLAIISREILADMREKFHSKSSKS
GAKRVHYLCMEFLLGRNLKSTLFNLGLDKIYTKILKSINIDIEDVYEVENDAGLGNGGLG
RLAACFMDSLATLNYASMGHSILYEYGLFKQKIVDGEQIELTDSWRSTGDFWLQKRQDKS
VIVRFGGNVIENMVDGKLVPTYENYTEVQAIPYDMILSGFDSQGVAVLRLWEAKSINKFD
LNSFSQGAYVKAVAEASEIEMISKVLYPADDHYEGKTLRLKQQYFLVSSALQNIVSTHLK
RYKNLSTLSDMTVIHINDTHPALCIPELMRILMDDYGWEWNSAWRLVNKTINYTNHTVLM
EALEKWDENLFKTILPRIYQIVKEINRRFTKDLLDNRSDEFDLSTVEKMSIVSQGQVRMA
NLSVIGSNHVNGVSKLHSDIIKNSLFKDFSRLYPNKFTNVTNGIAHRRWLCQSNPNLTEL
IDGSIGTNYYYDANDLKKLTKYSEDKTVLSKLNKIKQKNKKEFAEYLRKTRGITIDPNMR
FDVHIKRIHEYKRQLLNVLKIIYLYSELLDSPNKDVTPQVFFFGGKSASKYNMAKRIIKL
ICKLSQEIDKNPVVSAKLKVVFLENYNVSLAEKIIPAAEVSEQISLAGKEASGTGNMKFM
INGALTLGTFDGANVEMSECVGNDNIFIFGLSSEEVDNAWKVGYTPKEIYLNNPKVHKVI
EMLKVGFDGEKFDDIANYLIEGGRPDPYMCLIDFDSYLNCYHKMDETYKDSMVWAKMSLQ
NIANSGFFAADRSIEDYANNIWNLKPVK

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	95	804	7.5e-258	0.9955489614243324

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	63	808	70	814	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	6	806	1	750	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	46	805	42	797	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	25	804	12	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	14	804	4	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVQ97458.1	1.59e-288	10	807	10	806
AYF42955.1	1.59e-288	10	807	10	806
QCN93713.1	1.59e-288	10	807	10	806
AYF40115.1	1.59e-288	10	807	10	806
ADU27514.1	5.71e-288	10	807	47	843

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2FFR_A	1.20e-233	12	806	14	818	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
3ZCP_A	2.16e-233	12	806	26	830	Rabbitmuscle glycogen phosphorylase b in complex with N- cyclohexancarbonyl-N-beta-D-glucopyranosyl urea determined at 1.83 A resolution [Oryctolagus cuniculus]
2GJ4_A	2.32e-233	12	806	14	818	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2GM9_A	2.40e-233	12	806	14	818	Structureof rabbit muscle glycogen phosphorylase in complex with thienopyrrole [Oryctolagus cuniculus],5MCB_A Glycogen phosphorylase in complex with chlorogenic acid. [Oryctolagus cuniculus],7ONF_A Chain A, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
7O8E_A	2.83e-233	12	806	19	823	ChainA, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39123	1.53e-232	27	807	25	797	Glycogen phosphorylase OS=Bacillus subtilis (strain 168) OX=224308 GN=glgP PE=2 SV=1
P79334	1.62e-232	12	808	26	832	Glycogen phosphorylase, muscle form OS=Bos taurus OX=9913 GN=PYGM PE=1 SV=3
P00489	2.36e-232	12	806	26	830	Glycogen phosphorylase, muscle form OS=Oryctolagus cuniculus OX=9986 GN=PYGM PE=1 SV=3
O18751	1.30e-231	12	808	26	832	Glycogen phosphorylase, muscle form OS=Ovis aries OX=9940 GN=PYGM PE=2 SV=3
Q9WUB3	7.35e-231	14	806	28	830	Glycogen phosphorylase, muscle form OS=Mus musculus OX=10090 GN=Pygm PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001050_00316.