CAZyme Information: MGYG000001063_02241

Basic Information

• Species: Robinsoniella sp900540475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900540475
• CAZyme ID: MGYG000001063_02241
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2024	MGYG000001063_60|CGC4	222616.46	4.6577

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001063	7637037	MAG	Sweden	Europe

• Gene Location: Start: 153474; End: 159548 Strand: +

Full Sequence      

MCNRFWKKRKQGLALVLAVVMSVVSLEAPVFAAEVQNMEIEADTSRIAEDTQDTTIYDLK
TDDLVNPVGIDTANPVFSWKMNAATMGQCQTAYQIIVAKDVEFADAVWDSQKQLSDISVG
IKYAGNPLEASTTYYWKAVVWDKDGKEIQSDTASFEMGLLGNEGWNQSQWIQVGNSTEPP
ISTAGETNYVVEADIQIVTTSVSLLLEATDNNNFLLWQFNNKNGYMEFKPHYKKAGNFHT
VRQMNVSPYINGKDTDVQHLKVVATNESISTYLNDHLIEEIPASELGGIGLNGTIGRLGF
RSHSLEDEAGWMDNITLTDYSVDPDGIVLKKYDFEDGYNPFDDGAVENGRLSTKYTGEET
IALEKTEEETVEKVHYSVEADITCLTDAVSLLFNAADTSNFYMLQLNTKDQPGKVIFKPH
TWKDGNFATYSSHNKDVTAYLGSAEEFKSNPAHVKIDVTEKEIQTYLNDQLIDTFSIGEL
SDQNSKGIEPQIGHLGFRADLEENGTVDNLRLVDYTDNMGGEIVYDYDFDHDNPFLSGAV
EEGRFVTKGVGILLPPQGISTFRKEITPGDNLVSAKLYTAGLGVYDVFINGERVGTRQDD
GSVVFDELKPGYTHHSKRTIYHTYDVTQMVNGEEASVISAHVTSGWWSGQVAGFYGKEEA
FRAQLLLTYEDGSTRVIGTDRSWKTALQGPILYGDIYNGETYDANADLSFRQTGYDDSKW
TYADLNKEFNGILCPQDGPSVRVRNDLELTAQSAVVYRGVVDENENQFGRINITGNYAPV
SGFILKAGETAVFDMGQNFAGWDEIQAEGKKGTILTMRHSEMLNDNNGLKSRGNDGAQGS
IYTANLRSAKAAGRYIMSGDGIESYHSTSSFYGFRYVEVTTTQDVTIHGMKGIVVSSVTD
DTGKIATSDDDVNQLISNILWGQYSNYLSVPTDCPQRDERKGWTADTQVFSSAAAYNADS
KGFLRKYMDDMNDSQVTEGEYNGAYPDTAPYNGYGEIGQLGWGDAGIIIPYNLYKMYGDA
TVIEENYSNMQDFMDIFMASTNKMGGGHNHGDWLAYESNDDDIQNLFGIAYYAWDAAMMS
EMAAVLGKTEDAMRYQALYEEEKAFFQEMFVQEDGSLKRTEQTACLMALKMDLLPDENSK
AVVKQALLDNIKRNGNKLQTGFLGTAIIMQTLSDIGATDVAYQLLLQHGNPSWLYSVDQG
ATTVWERWNSYTIEDGFGPVSMNSFNHYAYGAVAEWMYGYMAGIMYDTQNPGFKHMILQP
SPDQSIQKVDCTYDSAYGSIVSNWSYQDAKFNYDAVIPANTTATILIPVEDGETVSVNGK
SSAEVTAEKDGLSYIETKDNKAVFEAVSGSYHFSTGVAQYCNITLKNADSTIPCLISVDG
SEMQVMPSGIKVEKGKSITIKAVPVNDIDYACVGWSGDESADGSQITVTPLNNMTLAAEF
AWSGSENLAWQQPVTSNETGWDIDAWSHANLVDGILTSESGSLGYTTMQGQSPEVDYWVE
IDLGEDMDFNRFQLYPRSDTLSINGGAPNFPKDFSFEVRKEDETQYNTIVSNTDYKAAVG
KPSVFTFEAVNARYVRLHVTKLGDPAAADRDYYFLQLAEMGVYNRDNIPVIDKAVLEKAI
GSAKTYDGKQADYTESSWKDFQEALEEAKRILRDETADQETVDTAAQALNQAMKDLKEAD
REPVVESVKVSPGSTVLERGKTQRFTASVMGKNEPQQTVTWSVTGNHSGATTISNDGVLT
VGMDETAALLTVRAVSEADHDKFGTATVTLKVLPPSDVKVNHISVTASSNRIFVKDKTSV
RAVVLPESATNKNFNWTSSDTKVATVDSQGKVTAKKDGTVRITATAADGSNVSGSCTIKV
VKPRVKLNASSIKLQLKKSTKALKASGLLSGDKIKSWTSKNKKIATVTKSGKIIAKKAGN
TSIIVTTVKGAKAICRIKVVKSAVKTNKITADKKKVTLKRGKSYQLKITRSPITATDKIT
YTSSADRIVSVNKKGKIAAKKKGRVEITIKTSNGKSTKVKVEVV

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	788	1305	3.5e-155	0.9821428571428571
CBM67	555	724	1.4e-24	0.875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	1.64e-124	900	1241	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	3.70e-47	574	745	5	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	2.01e-26	790	886	7	97	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
COG5492	YjdB	2.73e-16	1775	1927	176	321	Uncharacterized conserved protein YjdB, contains Ig-like domain [General function prediction only].
pfam17390	Bac_rhamnosid_C	6.13e-14	1251	1320	7	76	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCA51920.1	4.79e-254	56	1353	21	1145
ADD61991.1	1.32e-253	56	1353	21	1145
QUT40529.1	1.84e-253	56	1353	21	1145
QQA09206.1	7.09e-253	56	1353	21	1145
ALJ43908.1	5.34e-252	56	1353	21	1145

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	1.30e-133	67	1358	17	1033	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6GSZ_A	1.04e-108	478	1317	31	856	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
6I60_A	7.28e-99	562	1353	179	939	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	1.33e-132	67	1354	17	1029	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	1.66e-129	535	1353	139	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	1.81e-114	538	1321	152	892	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	1.67e-72	560	1321	185	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
P33747	5.05e-13	1783	1919	40	179	Uncharacterized protein CA_P0160 OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_P0160 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000412	0.998794	0.000238	0.000216	0.000178	0.000156

TMHMM  Annotations     

start	end
13	35