CAZyme Information: MGYG000001097_00855

Basic Information

• Species: Prevotella pectinovora
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella pectinovora
• CAZyme ID: MGYG000001097_00855
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
704		78692.29	7.9476

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001097	3372203	MAG	China	Asia

• Gene Location: Start: 3155; End: 5269 Strand: +

Full Sequence      

MAKKIIHSIIPLRGLGLMAALSMAVLPSAAQKNGITDTGKSKYAVVSSTPLSAVRWTGGF
WGERFGVFSGTSVQSMWETWKSDTGHGWNNFLIAAGEKKGKRHGPPFHDGDMYKWLEALT
AVYAVNKDPEVGKIIDRFVELIQKSQRPDGYLHTPVIIAEMNRQQSAKEEAKENETIGTK
VGTGKDGAFGNRLNFETYNLGHLITAGIMHKRATGKNTLFNCAVKAADFLYDFYKRASDE
LARNAICPSHYMAVTEMYRETGDPKYLELSEKLIDIRGKVENGTDDNQDRVPFRQQYNAM
GHSVRANYLYAGVADLYLESGEEQLKKNLDAIWTDIVTRKMYITGACGALYDGTSPDGTD
YKPDNVQKVHQSYGRPYQLPHSTAHNETCANIGNLFFNWRMFAATGDAKYTDVVENCLYN
SILPGISLDGKKYFYTNPMRISADLPYKLRWPKERTEYISCFCCPPNTLRTLCEAQDYAY
SIGKNEIYVNLYGDNTLNTRIDGIGDIAISQTTDYPWDGTVELTIDKLKGKKQFGIRLRV
PDWCTEGATILVNGAQQTIEAAAGTYATVTRTWKKGDVVTLSMPMRTRLIEANPLVEESR
GQVAVQRGPIVYCLESNDLGGARIDDIAIPLNAKFTPVDMTIDGSRVKALEAEVINRSEP
SWKGQLYREATAKKHTQTVRFIPYYAWGNRGKSEMTVWIPASLD

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	54	613	3.2e-184	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	53	613	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	3.29e-136	50	701	13	587	Uncharacterized conserved protein, DUF1680 family [Function unknown].
COG1331	YyaL	0.003	197	276	410	499	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUR43102.1	0.0	17	700	7	695
QRN01927.1	0.0	17	700	7	695
QNL37669.1	0.0	17	700	7	695
QUT70969.1	0.0	22	701	12	696
QDH55672.1	0.0	17	700	7	695

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQO_A	0.0	22	700	12	695	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
4QJY_A	2.51e-120	60	699	22	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	4.93e-116	60	699	22	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	2.35e-108	46	704	7	628	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
7EXU_A	1.37e-81	86	697	52	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.59e-80	86	697	52	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.005320	0.990496	0.003208	0.000416	0.000280	0.000259

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001097_00855.