CAZyme Information: MGYG000001109_00666

Basic Information

• Species: CAG-110 sp900544975
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-110; CAG-110 sp900544975
• CAZyme ID: MGYG000001109_00666
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2438		262557.26	4.1609

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001109	2762977	MAG	China	Asia

• Gene Location: Start: 6719; End: 14035 Strand: +

Full Sequence      

MKENCLKRLLSFALVIAMVVSLCPTIAFAGDTLPYRGESALADNEPTSHGYRAEDLLSWD
PATDPDAELLRARVPLQNRIAAVAATQANPSLNPEVEYLTLAGDYGNAFFGGTSYTNEFS
EYCFNFWQYIDYYGPWHGQTTATTPIELWDVDDERVNNDAWKNRSFEFGMMNLPNAAYTN
AAHKNGVLSLGCIFLPRAYQSWRTLIQRDENGGFPYADKLIEITNYYGFDGWFVNLEGSD
SPSGTAKQELAAFLKYLREEGKLYIQYYNAGGSIDTQMMDSGAADSFFKDYGWSTAGAKN
ETAKYGIGAVHGGFEAGGNRWNNNFSKMWQDGEIVCSIASLGTDFVHAGIDEDFGNGKQM
RREDDDFQWMSAIRERMWFTGSSGNPTDSSTKSNAEVGVSRNSMPGIASLVTERSVISGD
TFVTNFNTGHGLVYAKNGEVSNSHEWANISIQDILPTWQFWFESEGTALQAEYDYGTTYK
KGLTAQWNQGTLVEGSTTYGEFDFDLVNPYEGGSSLAVYGKVDADNYMRLYKTNLDVTAN
TQMQVTFQKTSSDNVAMSLGLVFAEDINAEDNTYTVTNIPLENTTAVSDGWVTATADLSA
YAGKQVAVIGLNFQGTADNYQMHIGNISYTSGKDLTPAAPTGLTIDKAFDTNEMVVSWDL
ADYDTVKQYNVYAVINGVEQYMGGIYDDIYYIKNVYDAQGEVTIKVKAVGADGAESEAAV
ATYDYSKAVTGLTVTPADGTLTVNFTPADAAAATDVSVYVPATGKTYTAQAAAGETSVVV
AVPAGAEADGREYEMQVTPVGGSPKAYDGDLDDSYCIPYDGPMTGHQLTSPVACTDWYKL
ALSYTTRSGGAGSTEYTRGVRSHGELNHDWSRFQSLPSDILTLEVTLTDYDGNVSEKVTY
NFDDQGEPIIPEVFDRVFHLDAGRKYFSVQYIKQILDTMAEANFNQLELYFSDNQGFRFA
LEDMTVTTEFGTYDLTPSLGDGYVGDNKYPDNSGKYLTQSEMDEIISYANSKGIELVPAL
NVPGHMGAILEEFPDLRYTGSKSSIDLTSDEAKAFAKAIVEKYAEYFASKGCKHFNFGAD
EFANDLGSMGFDVIYKNGIYKEHFVPFFNEVAAIIKDKGMTPRAFNDGVMYNNDTSVEMD
RDVQITYWSSGWGGYSLASATTLNDAGFELINTNQGYYWVLGNADWQVSVKKAGEFDCTE
FEGGDVIYKPAGAMLCVWCDNGAADGLDDGKGVAEKIADVIKAFGSTLPEVRNMEPLVDE
TYFPDAALRQAVISQVGPYVTDLSGFTGVLNCANTNVADLTGVREYLPGLTGLNLAGTKM
TKVTSSMLPSGLKTLDLTNCKDLTFVELEGHPNLVVNFTGCTAVENLYLSGTNMTEIDIS
AMTRLHNFVISNSQISMITAANASAYTNAYYWNWQNAKLDLSAGTKEGKLKDGVESYFNT
ADLEEEINPVASRVGSGSLSYWGGNSTTINLNRVVPLSTIQVSNPYANWGYYEVLTKMTV
EVSSDGTNYTVAAEVTDATADYTVELPEGVRAQYIRITNTDGIDCYASFTVYGHEVAPMG
YTYNGQQPALVRDEMSDVIYERNGQQYQLLDLLNENYKTYRTVRGTLVADLEGESWVDAA
YLTNATVVPKGVKVVITDENGETYVPSSVEEPDLGTLDETVNVALNASVQGQSAQYTGEE
PYHLFDGIISGSSNKWCGRGATAWVGFTLPESVVVGKWFSVHAKGELESYITQDFSLQVL
DPNGAISEEDFLEMSSNEKRSVMSNNSYWVDLDSVTGNTDVEVTRNIPQENLIQAQVYRL
YVGKSTAGNDNNVRINELELYAYTGALKADTNGVFVADEIGTWNVSYQKAGQELANVNVR
VRLSDQDLVELSELVESAKNDAEAAKTAAEAAQAQAEAAKADAEAAKAEAKAAQTAAEQA
AAEAGKDRTAAEAAKVKAEEAQAKAEAAQAAAEEAQGKATAAQTAAEAAQAAAQTAAEAA
EQSNREAAMKAAEAAGSAKAAAEAAAKAAFSAAEAAASAEAAAGSATEAAKSMVEAQKAQ
AAAEDAQVKAEEAKKKAEEAQKKADEAAQSAAENKESAEKAAAEAEAAQEAAAKAQKAAE
DARAAAETARNAAEEANREAAAAAAEAAAYAKQMADTYAEIVAIKAEIVEYLAQAQKAAE
DAEAAKKAAEEAQKKVEEALKKAQEAALTAAKYGAIVELVGIDVSGYTAEQMELVDAALA
TAREAVAAAETVEEVEAALAVAKEAILAAGQLVCASDLFRDVAENAWYHKGVDFVVRNGY
MEGMGNGIFGVNAAITRGQMVTILYRMEGKPSVEGLENVFTDVADGKFYTDAVIWAASKG
IVEGNGDGTFNPNGAITREQLATILYRYDGKQAVTEDALAGFPDGKSVSPFAVEAMNWAV
ANGLIHGIGNGSSATLSPKATASRSQFATILMRYMTEG

Enzyme Prediction     

No EC number prediction in MGYG000001109_00666.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	916	1224	3.7e-54	0.884272997032641
GH85	109	434	1.1e-51	0.9746031746031746

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06564	GH20_DspB_LnbB-like	8.45e-74	915	1224	2	301	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
COG4724	COG4724	4.58e-72	44	628	27	548	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
pfam03644	Glyco_hydro_85	7.76e-56	116	431	2	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
cd06547	GH85_ENGase	8.40e-51	100	466	1	339	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
cd02742	GH20_hexosaminidase	3.91e-40	915	1225	1	283	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNO18499.1	1.49e-247	6	899	13	917
QDH22151.1	7.74e-185	8	896	17	926
APB70257.1	2.24e-184	13	893	15	908
AET57459.1	2.24e-184	13	840	15	854
ALA43266.1	4.25e-184	13	893	15	908

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4AZ7_A	3.79e-60	916	1224	8	343	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
2YL6_A	3.89e-60	916	1224	8	343	Inhibitionof the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis [Streptococcus pneumoniae TIGR4],4AZ5_A Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
4AZB_A	3.89e-60	916	1224	8	343	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
4AZ6_A	3.99e-60	916	1224	10	345	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
2YLL_A	6.50e-60	916	1224	28	363	Inhibitionof the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P49610	5.84e-52	915	1225	632	958	Beta-N-acetylhexosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=strH PE=1 SV=2
B2UPR7	3.60e-12	916	1181	235	492	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1
Q04786	1.51e-11	911	1179	313	639	Beta-hexosaminidase OS=Vibrio vulnificus OX=672 GN=hex PE=3 SV=1
A1L251	1.56e-10	166	306	142	299	Cytosolic endo-beta-N-acetylglucosaminidase OS=Danio rerio OX=7955 GN=engase PE=2 SV=1
P38537	2.62e-10	2253	2433	27	204	Surface-layer 125 kDa protein OS=Lysinibacillus sphaericus OX=1421 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000412	0.998774	0.000276	0.000195	0.000173	0.000148

TMHMM  Annotations     

start	end
9	28