CAZyme Information: MGYG000001112_01339

Basic Information

• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pasteurellaceae; Haemophilus_D
• CAZyme ID: MGYG000001112_01339
• CAZy Family: GT25
• CAZyme Description: Lipooligosaccharide biosynthesis protein lex-1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
282		33212.33	7.1079

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001112	2020645	MAG	China	Asia

• Gene Location: Start: 79927; End: 80775 Strand: +

Full Sequence      

MYSIEHKNTVISISTADKRRNHIIDQFGQKQIPFEFFDAFTPSDRLDAHLQRYLPHVDAT
SKLTAGEKGCLMSHFMLWKKCVDDNLDYISIFEDDILLGENAEQFLANDEWLKMRFNFQE
IFVLRLETFLMPVQLEHQQQILPFQERNIDILKSKHFGTAGYIISNGAAKYLINLFEKLA
VEDIKAIDEIMFNELINVGVYQVYQLNPAICVQELQLNQENSLLVSDLQQERKKNTAVRT
KKTLKQRLTRIKENIIRALNKEKWKEQQRLKEIQGKEIVRFM

Enzyme Prediction     

No EC number prediction in MGYG000001112_01339.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT25	7	191	3e-37	0.9834254143646409

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3306	COG3306	4.48e-62	7	268	4	255	Glycosyltransferase involved in LPS biosynthesis, GR25 family [Cell wall/membrane/envelope biogenesis].
pfam01755	Glyco_transf_25	6.77e-57	6	191	2	200	Glycosyltransferase family 25 (LPS biosynthesis protein). Members of this family belong to Glycosyltransferase family 25 This is a family of glycosyltransferases involved in lipopolysaccharide (LPS) biosynthesis. These enzymes catalyze the transfer of various sugars onto the growing LPS chain during its biosynthesis.
cd06532	Glyco_transf_25	4.32e-31	7	191	1	128	Glycosyltransferase family 25 [lipooligosaccharide (LOS) biosynthesis protein] is a family of glycosyltransferases involved in LOS biosynthesis. The members include the beta(1,4) galactosyltransferases: Lgt2 of Moraxella catarrhalis, LgtB and LgtE of Neisseria gonorrhoeae and Lic2A of Haemophilus influenzae. M. catarrhalis Lgt2 catalyzes the addition of galactose (Gal) to the growing chain of LOS on the cell surface. N. gonorrhoeae LgtB and LgtE link Gal-beta(1,4) to GlcNAc (N-acetylglucosamine) and Glc (glucose), respectively. The genes encoding LgtB and LgtE are two genes of a five gene locus involved in the synthesis of gonococcal LOS. LgtE is believed to perform the first step in LOS biosynthesis.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOR23453.1	1.04e-186	1	282	1	282
AFJ20733.1	1.48e-186	1	282	1	282
QOR20037.1	6.02e-186	1	282	1	282
QUC06110.1	2.16e-156	1	282	1	282
AKU62975.1	4.35e-156	1	281	1	281

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q03974	3.92e-108	7	282	5	302	Lipooligosaccharide biosynthesis protein lex-1 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=lex1 PE=1 SV=1
Q50950	2.86e-61	7	281	2	279	Lacto-N-neotetraose biosynthesis glycosyltransferase LgtE OS=Neisseria gonorrhoeae OX=485 GN=lgtE PE=3 SV=1
Q57394	4.25e-60	6	245	2	235	Lipooligosaccharide biosynthesis protein lic2B OS=Haemophilus influenzae OX=727 GN=lic2B PE=3 SV=1
Q51117	5.76e-58	7	281	2	279	Lacto-N-neotetraose biosynthesis glycosyltransferase LgtE OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=lgtE PE=3 SV=2
P57033	3.15e-57	7	281	2	278	Lacto-N-neotetraose biosynthesis glycosyltransferase LgtB OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=lgtB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000082	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001112_01339.