CAZyme Information: MGYG000001113_00316

Basic Information

• Species: Duodenibacillus sp900552545
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; Duodenibacillus; Duodenibacillus sp900552545
• CAZyme ID: MGYG000001113_00316
• CAZy Family: GH102
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
470	MGYG000001113_17|CGC1	50553.53	9.7165

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001113	2010666	MAG	China	Asia

• Gene Location: Start: 7051; End: 8463 Strand: +

Full Sequence      

MKKTAGRFETILAKGRLAALAGAAAALLAACSSVPETTSTTTGAPNVIEGAALPDQAETR
SATTAGTTISGAALWPSSSARPAAGEAAYPLADGAVRFGKSSFAELPPVTDESWEAALAA
FKLSCSKMGTGAMWLDACSNAQFVGEGRAREFFLIWFDLWRVAADTRATNASNNAGIVDT
GLATGYYEPLLRGSRTRHGPYQYPIYRVPDDLIAVELDALYPQLKGLRLRGKLVGGKLVP
YDSRAEIERRARLGQMQRYALCWVDDPVEAFFLQIQGSGRIALEDGTYMRVGFADQNGHP
YKAVAKWLAENAGLSLSEMSMQRIKRWAKENPQRTQELLNANPNFVFFAERKGGDPELGP
IGAQGVPLTAQASVAVDRRYWPLGLPFVIDVKQTRPAQAFTRAVVAQDTGSAIRGVLRFD
YFWGFGEAAAGPAGAQKSAVRAWILMPKGEDPRRRMQTKAAGSGATAPRI

Enzyme Prediction     

No EC number prediction in MGYG000001113_00316.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH102	191	348	3.1e-58	0.9872611464968153

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam03562	MltA	8.19e-98	110	348	2	230	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
COG2821	MltA	9.56e-86	59	455	2	373	Membrane-bound lytic murein transglycosylase [Cell wall/membrane/envelope biogenesis].
cd14668	mlta_B	6.93e-81	191	351	1	159	Domain B insert of mltA_like lytic transglycosylases. Escherichia coli MltA is a membrane-bound lytic transglycosylase comprised of two domains separated by a large groove, where the peptidoglycan strand binds. Domain A is made up of an N-terminal and a C-terminal portion, which correspond to the 3D domain, named for 3 conserved aspartate residues. Domain B is inserted within the linear sequence of domain A. MltA is distinct from other bacterial lytic transglycosylases (LTs), which are similar to each other. Escherichia coli peptidoglycan lytic transglycosylase (LT) initiates cell wall recycling in response to damage, during bacterial fission, and cleaves peptidoglycan (PG) to create functional spaces in its wall. PG chains (also known as murein), the major components of the bacterial cell wall, are comprised of alternating beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), and lytic transglycosylases cleave this beta-1-4 bond. Typically, peptidoglycan lytic transglycosylases (LT) are exolytic, releasing Metabolite 1 (GlcNAc-anhMurNAc-L-Ala-D-Glu-m-Dap-D-Ala-D-Ala) from the ends of the PG strands. In contrast, MltE is endolytic , cleaving in the middle of PG strands, with further processing to Metabolite 1 accomplished by other LTs. In E. coli, there are six membrane-bound LTs: MltA-MltF and soluble Slt70. Slt35 is a soluble fragment cleaved from MltB. Bacterial LTs are classified in 4 families: Family 1 includes slt70 MltC-MltF, Family 2 includes MltA, Family 3 includes MltB, and Family 4 of bacteriophage origin. While most of the LT family members are similar in structure and sequence with a lysozyme-like fold, Family 2 (including mltA) is distinct.
smart00925	MltA	3.06e-73	190	348	1	152	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
PRK11162	mltA	9.23e-45	182	415	117	325	murein transglycosylase A; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF22454.1	1.85e-136	102	453	46	391
QQS89395.1	1.78e-131	90	469	39	405
QDA55021.1	2.17e-129	98	452	48	391
ANU66434.1	1.39e-121	103	452	50	396
QQQ97583.1	1.39e-121	103	452	50	396

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7ESJ_A	4.57e-86	90	449	16	361	ChainA, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii],7ESJ_B Chain B, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii]
6QK4_B	6.31e-81	80	447	8	346	Lytictransglycosylase, LtgG, of Burkholderia pseudomallei. [Burkholderia pseudomallei]
2G5D_A	1.44e-72	99	452	38	418	Crystalstructure of MltA from Neisseria gonorrhoeae Monoclinic form [Neisseria gonorrhoeae FA 1090]
2G6G_A	6.12e-71	99	452	38	418	Crystalstructure of MltA from Neisseria gonorrhoeae [Neisseria gonorrhoeae FA 1090]
3CZB_A	5.94e-39	102	450	11	344	Crystalstructure of putative transglycosylase from Caulobacter crescentus [Caulobacter vibrioides CB15],3CZB_B Crystal structure of putative transglycosylase from Caulobacter crescentus [Caulobacter vibrioides CB15]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0A935	6.61e-32	184	445	119	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli (strain K12) OX=83333 GN=mltA PE=1 SV=1
P0A936	6.61e-32	184	445	119	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli O157:H7 OX=83334 GN=mltA PE=3 SV=1
Q8K9A7	7.65e-24	184	445	127	364	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=mltA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000103	0.003304	0.996614	0.000018	0.000006	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001113_00316.